11745832910 Roche标记检测试剂盒I 现货促销

Roche标记检测试剂盒I 现货促销。比普通DIG随机引物法标记效率高, 操作简单, 可用于Southern、Northern和斑点杂交以及菌落和噬菌体筛选。一个Kit可用于12次标记反应和24次检测反应。组成: 5×DIG-GX标记试剂; 未标记对照DNA, PBR328; DNA稀释液; 抗-DIG-碱性磷酸酶复合物; NBT/BCIP储存液; 10×封闭液; DIG定量复试纸条; DIG对照试纸条, -20℃保存。Roche标记检测试剂盒I 现货促销

别名: 

Roche:  DIG-High Prime DNA Labeling and Detection Starter Kit I DNA标记和检测试剂盒I型(显色法)

比普通DIG随机引物法标记效率高，操作简单，可用于Southern、Northern和斑点杂交以及菌落和噬菌体筛选。 一个kit可用于12次标记反应和24次检测反应。

组成：5×Dig-GX标记试剂；未标记对照DNA，PBR328；DNA衡释液；抗-Dig-碱性磷酸酶复合物；NBT/BCIP储存液；10x封闭液；Dig定量试纸条；Dig对照试纸条。 -20℃保存。

Properties

Related Categories	12 Principles Aligned Products, Chemical Synthesis,Greener Alternative Products, Life Sciences
usage	sufficient for 12 labeling reactions
	sufficient for 24 blots
mfr. no.	Roche
greener alternative product characteristics	Designing Safer Chemicals Learn more about the Principles of Green Chemistry.
shipped in	dry ice
storage temp.	−20°C

研究领域: 

脱氧核糖核酸

其他

详述

Description

Application

DIG-High Prime is used for the highly efficient random-primed labeling of DNA probes with DIG-11-dUTP, alkali-labile. DIG-labeled probes are generated at high yield within one hour or after overnight incubation. DIG-High Prime-labeled DNA probes are used in a variety of hybridization techniques: • Southern blots
• Northern blots
• Dot blots
• Colony and plaque hybridizations
• For all types of filter hybridization
• For single-copy gene detection in total genomic DNA, even from organisms with high complexity, for example, human, barley, and wheat

The DIG High Prime DNA Labeling and Detection Starter Kit I uses digoxigenin (DIG), a steroid hapten, to label DNA probes for hybridization and subsequent color detection by enzyme immunoassay. The "random primed" DNA labeling method originally developed by Feinberg and Vogelstein is based on the hybridization of oligonucleotides of all possible sequences to the denatured DNA to be labeled. The input DNA serves as a template for the synthesis of labeled DNA, and is not degraded during the reaction, making it possible to label minimal amounts of DNA (10 ng) with this method. The complementary DNA strand is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. Modified deoxyribonucleoside triphosphates, labeled with digoxigenin present in the reaction are incorporated into the newly synthesized complementary DNA strand.

General description

Download the CofA, SDS and IFU specific to your product lot and format

Convenient kit for random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and color detection of the DIG-labeled hybrids. In this method, the complementary DNA strand of denatured DNA is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers.This kit was assembled with convenience in mind, offering a ready-made blocking solution, combined stock solution of NBT/BCIP, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow Enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.

Sample materials

• DNA fragments of at least 100 bp
• Linearized plasmid, cosmid or ?DNA
• Supercoiled DNA

Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Packaging

1 kit containing 7 components.

Quality

Function tested in a dot blot.

Roche标记及检测相关试剂盒

名称	编号	产地		规格	价格￥
DIG DNA Labeling and Detection Kit DNA标记和检测试剂盒	11093657910	Roche		Kit	8732
	DIG-11-dUTP随机引物法标记DNA, 碱性磷酸酶(AP)生色反应检测, 标记探针用于Southern、Northern和斑点杂交以及菌落和噬菌体筛选。组成: 未标记对照DNA1; 未标记对照DNA2; DNA稀释液; DIG-标记对照DNA; 六核苷酸混合物; Klenow 2u/μl; 封闭液; BCIP/NBT; 10×dNTP标记混合物; 抗--碱性磷酸酶复合物。一个Kit可用于25次标记反应和50次检测(100cm2膜), -20℃保存。
DIG-High Prime DNA Labeling and Detection Starter Kit I GXDNA标记和检测试剂盒I	11745832910	Roche		Kit	5457
	比普通DIG随机引物法标记效率高, 操作简单, 可用于Southern、Northern和斑点杂交以及菌落和噬菌体筛选。一个Kit可用于12次标记反应和24次检测反应。组成: 5×DIG-GX标记试剂; 未标记对照DNA, PBR328; DNA稀释液; 抗-DIG-碱性磷酸酶复合物; NBT/BCIP储存液; 10×封闭液; DIG定量复试纸条; DIG对照试纸条, -20℃保存。
DIG-High Prime DNA Labeling and Detection Starter Kit II GXDNA标记和检测试剂盒II	11585614910	Roche		Kit	5598
	比普通DIG随机引物法标记效率高, 采用CSPD化学发光检测灵敏度更高。一个Kit可用于12次标记反应和24次检测反应。组成: 5×GXDIG标记复合物; 未标记对照DNA, PBR328; DNA稀释液; 抗-DIG-碱性磷酸酶连接物; CSPD; 10×封闭液; DIG定量试纸条; DIG对照试纸条, NBT/BCIP储存液; -20℃保存。
DIG DNA Labeling Kit DNA标记试剂盒	11175033910	Roche		40T	7753
	随机引物扩增法以DIG-11-dUTP标记DNA; DIG标记探针可用于各种膜杂交。
DIG Nucleic acid Detection Kit 核酸检测试剂盒	11175041910	Roche		Kit	5165
	NBT/BCIP显色法检测, 灵敏度可达0.1pg同源DNA; 可检测40张100cm2杂交膜。-20℃保存。
DIG Oligonucleotide 3’-End Labeling Kit 寡核苷酸3’末端标记试剂盒	3353575910	Roche		25T	7432
	通过末端转移酶以DIG-11-ddUTP标记14-100bp的寡核苷酸探针3’端用于各种膜杂交和原位杂交。每个反应可标记100pmol的寡聚核苷酸。
DIG Oligonucleotide Tailing Kit 寡核苷酸加尾标记试剂盒	3353583910	Roche		25 T	6266
	通过末端转移酶以DIG-11-ddUTP在寡核苷酸探针3’端加一平均长度50bp(10-100bp)的寡核苷酸, 用于DNA/RNA膜杂交和原位杂交。每个反应可标记100pmol的寡聚核苷酸。
DIG RNA Labeling Kit (SP6/T7) RNA标记试剂盒(SP6/T7)	11175025910	Roche	2´10T		7592
	由SP6或T7 RNA聚合酶通过转录反应过程标记RNA, 1mg模板可产生约10mg标记RNA用于杂交。-20℃保存。组成: pSPT18 DNA, pSPT19 DNA, 对照DNA1, 对照DNA2, DIG标记对照RNA, NTR标记混合物, 转录缓冲液, RNaseYZ剂, 无RNase的DNase I, SP6 RNA聚合酶, T7 RNA聚合酶。
PCR DIG Probe Synthesis Kit PCR探针合成试剂盒	11636090910	Roche		25T	5598
	标记的探针可在Southern杂交后从5-10mg的人基因组DNA检测出单拷贝基因, 是从少量DNA模板制备大量探针的Z有效、Z经济的方法。
DIG Northern Starter Kit	12039672910	Roche		10T	5052
	包含标记DIG RNA及进行Northern印迹的所有必需试剂, 配合DIG Wash and Block Buffer Set方便进行Northern印迹; 能作10个标记反应及10次检测(100mm2膜)。特别适合初用者使用; 标记混合物经Z优化, 配合提供的化学发光底物CDP-Star, 能产生Z高的灵敏度。
DIG Luminescent Detection Kit 发光检测试剂盒	11363514910	Roche		Kit	5627
	CSPD化学发光法检测, 灵敏度可达0.03pg同源DNA; 可检测50张100cm2杂交膜。
DIG High Prime GXDNA标记试剂	11585606910	Roche		40T	7838
	标记DNA探针, 只需一步操作, 标记效率较通常随机引物法高3倍。40 T

储存与安全

储存: 

−20°C