CBP60665 |
I. General information |
Synonyms: | CFPAC-1 |
Species: | Homo sapiens, human |
Tissue: | pancreas; derived from metastatic: liver |
Disease: | ductal adenocarcinoma; cystic fibrosis |
Gender: | Caucasian,male,26 years |
Morphology: | epithelial |
Growth Mode: | adherent |
Doubling Time: | 32 hrs |
DNA Profile: | Amelogenin: X,Y |
CSF1PO: 10 |
D13S317: 12 |
D16S539: 9,11 |
D5S818: 10,11 |
D7S820: 8,10 |
THO1: 8 |
TPOX: 8 |
vWA: 17 |
Cobioer’s Cell Line Authentication Service |
Culture Medium: | MEM+10%FBS+1%NEAA+1%NaP |
We strongly suggest to purchase the complete medium from Cobioer. |
Cryopreservation medium: | Complete growth medium supplemented with 5% (v/v) DMSO |
Antigen Expression: | Culture medium, 95%; DMSO, 5% |
Tumor Formation: | Yes, in nude mice,Yes, in the cheek pouch of cortisone treated hamsters |
Comments: | The cells exhibit ion transport activities consistent with cystic fibrosis and express the product of the CF gene (cystic fibrosis transmembrane regulator, CFTR).CFPAC-1 cells show no effect of cAMP agonists, adenyl cyclase stimulators or phosphodiesterase inhibitors on Cl- flux, but do respond to Ca++ ionophores with increase Cl- efflux. |
The cells have the most common form of the CF mutation, deletion of three nucleotides resulting in the absence of phenylalanine at position 508.CFPAC-1 cells have epithelial morphology and polarization with apical microvilli, tight junctions and gap junctions. |
For more information, please contact Cobioer (4008-750-250). |