人虹膜色素上皮细胞

人虹膜色素上皮细胞             
            上海义森生物科技有限公司
虹膜是一个孔径可变，有颜色的圆盘状物，形成眼睛的瞳孔。鳞片状的上皮细胞覆盖其前表面， 双层有颜色的上皮细胞覆盖其后表面。虹膜色素上皮细胞与视网膜色素上皮细胞具有相同的功能特性，比如噬菌作用，降解杆体外节和合成营养因子。近年来，虹膜色素上皮细胞已被移植到视网膜下间­隙来ZL视网膜色素上皮细胞缺乏。移植后的巩膜色素上皮细胞可存活数月。移植后的IPE将与固有的RPE在视网膜下腔逐渐发生重塑。细胞的反应是巨噬细胞浸润，这与视网膜色素上皮细胞移植后表现相似。体外研究还表明，虹膜色素上皮细胞表达CD86，通过直接与细胞毒性T4淋巴细胞相关抗原相结合来YZT细胞活性。    人虹膜色素上皮细胞(HIPEpiC)提取于人虹膜组织，于原代冻存。每管含有细胞数>5×105 cells/ml，此细胞通过Cytokeratin-18, Vimentin和 Fibronectin免疫荧光染色验证，经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。培养至第二代时，细胞可以达到大约10倍增。
推荐培养基:(EpiCM, Cat. No. 4101)
产地：San Diego,US
品Pai：ScienCell
运输：干冰
用途：科研
The iris is a pigmented disk with a variable aperture which forms the pupil of the eye. It is covered by squamous epithelium on anterior surface and 2 layers of pigmented epithelium on posterior. The iris pigment epithelial cells (IPE) share functional properties with retinal pigment epithelial cells (RPE) such as phagocytosis, degradation of rod outer segments and synthesis of trophic factors [1]. In recent years, IPE have been transplanted into the subretinal space of eye to treat RPE defects. The grafted IPE were seen to survive for months. There was a remodeling of cellular layers in the subretinal space over time where grafted IPE joined the native RPE. The cellular response that developed exhibited macrophages and were similar to that observed following RPE transplantation [2]. In vitro study also show that IPE expressing CD86, directly suppresses T cell activation via binding to cytotoxic T lymphocyte-associated antigen 4 [3].
HIPEpiC from ScienCell Research Laboratories are isolated from human iris. HIPEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HIPEpiC are characterized by immunofluorescent method with antibodies to cytokeratin-18, vimentin and fibronectin. HIPEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HIPEpiC are guaranteed to further expand for two passages (around 10 population doublings) in the conditions provided by ScienCell Research Laboratories.
Reference
[1]. Semkova, I., Kreppel, F., Welsandt, G., Luther, T., Kozlowski, J., Janicki, H., Kochanek, S. and Schraermeyer, U. (2004) Autologous transplantation of genetically modified iris pigment epithelial cells: a promising concept for the treatment of age-related macular degeneration and other disorders of the eye. PNAS 99(20):13090-13095.
[2]. Crafoord, S. (2000) Experimental transplantation of retinal and iris pigment epithelial cells into the subretinal space. Student Journal of Health Sciences. 3(1):22.
[3]. Sugita, S. and Streilein, J. W. (2004) Iris pigment epithelium expressing CD86 (B7-2) directly suppresses T cell activation in vitro via binding to cytotoxic T lymphocyte-associated antigen 4. J. Exp. Med. 198:161-171.
储存：液氮