MBOAT7 (Lysophospholipid acyltransferase 7) ELISA Kit (CUS/ Mouse MBOAT7 (Lysophospholipid acyltransferase 7) ELISA试剂盒 (CUS

96 Tests

Operating instructions

FOR RESEARCH USE ONLY!

Alternative name

Intended use

Mouse MBOAT7 ELISA kit allows for the in vitro quantitative determination of Mouse Lysophospholipid acyltransferase 7 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

Mouse MBOAT7 ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MBOAT7, During the reaction, MBOAT7 in the sample or standard competes with a fixed amount of biotin-labeled MBOAT7 for sites on a pre-coated Monoclonal antibody specific to MBOAT7. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MBOAT7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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