RIN-m细胞,褐鼠胰岛素瘤上皮细胞冷藏ZX 货号:GD-C18919290
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞,其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Ethyl 4-nitrobenzoate, 98%
SodiuM Danshensu
Cycloleucine
2,1,3-Benzothiadiazole
(1R,2S,5R)-5-Methyl-2-(1-Methylethyl)cyclohexyl dihydroxy-acetate
N-(2-METHOXYETHYL)METHYLAMINE
N-Methyl 3-BroMo-5-MethylbenzenesulfonaMide
Cytosine ,99%
3-(Trifluoromethyl)benzoyl chloride, 98%
N,N-Dimethyl-1,4-phenylenediamine Dihydrochloride [for Biochemical Research]
(Perfluorocyclohexyl)methanol
2-Bromo-4'-Benzyloxy-3'-nitroacetophenone
ALUMINUM SULFIDE
4-Fluorophenyl sulfone
1-(2-BroMoethoxy)-2,4-dichlorobenzene
2-(2-Furyl)aniline
6,7-Dihydro-2-(2,4,6-triMethylphenyl)-5H-pyrrolo[2,1-c]-1,2,4-triazoliuM Perchlorate
4-BroMo-2-forMylthiazole
Methyl picolinate, 98%
Taurine, USP
TetrapropoxyMethane
2-Adamantanone
Basic Violet 16
N-Methylmethanesulfonamide
4-Hydroxyphenethyl alcohol
2,4-Difluoroanisole
DiMethyl trisulfide
6-Methoxy-2-Methylpyridine-3-boronic acid pinacol ester, 96%
6-Nitropyridine-2-carboxylic acid, 97%
n-Dodecyl thiocyanate, 97%
H-Lys(Boc)-OH
COBALT(II) CHROMATE
2-Chloro-5-fluoropyridine-4-boronic acid
N-TERT-BUTYLBENZENESULFENAMIDE
TRIMETHYL(2-PYRIDYL)TIN
4-(Methylphenyl)sulfonylacetonitrile
1-[(2-methyl-1,3-thiazol-4-yl)methyl](SALTDATA: FREE)
Ethyl 5-(2-bromoacetyl)isoxazole-3-carboxylate
2,5-Dimethoxy-2,5-dihydrofuran (cis- and trans- mixture)
1,4-Bis(3-hydroxyphenoxy)benzene
1-(2,3-Dimethylphenyl)
1-BROMO-2,4,6-TRIMETHOXYBENZENE
CADMIUM SULFATE
1,3-Diethylbenzene;M-Diethylbenzene;
2,4,6-Collidine
1-Butyl-3-MethyliMidazoliuM TribroMide
3-(2-Ethoxy-2-oxoethoxy)phenylboronic acid, pinacol ester
Dicarbonylacetylacetonato rhodiuM(I)
5-BroMo-2,1,3-benzothiadiazole, 97%
5-Hydroxyindole-3-acetic Acid
Methyl p-Anisate
Bis(ethylcyclopentadienyl)iron
7-bromo-2,4-dichloroquinazoline
4-(2-HYDROXY-PROPYL)--1-CARBOXYLIC ACID TERT-BUTYL ESTER
N6-Benzoyl-2'-O-methyl-adenosine
6-BROMOCOUMARIN-3-CARBOXYLIC ACID
Acetone oxiMe
YTTRIUM(III) 2-ETHYLHEXANOATE, 10% W/V IN N-HEXANE
TriMethoxyMethane
2,2,4-Trimethylpentane [for HPLC Solvent]
1-Chloro-4-iodo-2,5-dimethoxybenzene, 97%
2,4,5,6-Tetrafluoropyrimidine, 95%
3-Bromo-7-(trifluoromethyl)imidazo[1,2-a]pyrimidine ,97%
Bisacodyl
Quinoline-N-oxide
Chimassorb 944
3,5-Dimethoxy-4-hydroxybenzaldehyde
Phenylacetaldehyde
1-Butyl-3-MethyliMidazoliuM Tetrachloroferrate
2-Fluoro-4-(hydroxyMethyl)benzonitrile
CesiuM chloride, 99+%, for analysis
5-Nitro-3-phenyl-1H-indole-2-carboxylic acid, 97%
Methyl 2-(CyanoMethyl)benzoate
3-Hydroxy-3-phenylpropionic Acid
Hexyltrichlorosilane
Argireline
LITHIUM TETRAKIS(DIMETHYLAMINO)ALUMINATE
2'-deoxyadenosine 5'-triphosphate disodium salt
ECP ELISA Kit
SP ELISA Kit
EP ELISA Kit
Human DPP3 ELISA Kit
17-OHCS ELISA Kit
IFN-β/IFNB ELISA Kit
IgE ELISA Kit
Human C10 ELISA Kit
Human TF (Serotransferrin) ELISA Kit
Free-T3 ELISA Kit
Purple Agar Base
Korser’s Citrate Broth
Manganese(II) Acetylacetonate
CadMiuM fluoride, 99%
5-(Methylthio)thiophene-2-carboxaldehyde, 97%
2-Di-tert-butylphosphino-2',4',6'-triisopropylbiphenyl, 98%
2-Adamantanone
Dithienylethylene
Sodium antimonate
Arachidonic acid
1-Hexadecylamine
DIPHENYLTIN OXIDE
3-Chloro-2-Pyridinecarboxylic Acid
BisMuth hydroxide nitrate oxide, Bi{2}O{3} 79% Min
N-tert-Butyl-4-chlorobenzaMide, 97%
Nonafluoro-1-butanesulfonyl chloride
3,4-DiMethylbenzenethiol
(S)-2-(N,N-DibenzylaMino)-3-Methylbutanol
Triethyl 1,3,5-benzenetricarboxylate
Tubocurarine Chloride Pentahydrate, 98%
(R)-(+)-2-Methyl-2-propanesulfinaMide
(4S)-N-Crotonyl-4-benzyl-2-oxazolidinone, 99%
2-(Trifluoromethoxy)fluorobenzene
Tris(2,4-dimethylphenyl)phosphine, 98%
3-Nitrobenzamide