PAN-1细胞 人导管上皮癌细胞系更多培养技术指导 货号:GD-C19820548
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞 全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞 将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2-Chloro-6-fluoro-3-Methoxyphenol, 97%
2-BroMo-6-fluorobenzoic acid
ChloraMine-T trihydrate
(1S,2S)-(+)-2-AMino-1-phenyl-1,3-propanediol
2-BroMo-3-Methylnaphthalene
2,2-DIFLUOROCYCLOPROPANECARBOXYLIC ACID
2,3-Naphtho-15-crown-5
4-Aminophenyl β-D-thioglucopyranoside
4-(5'-Indole)benzoic acid
4-Chloro-5-methyl-6-phenylthieno[2,3-d]pyrimidine , 97%
Dimethyl Azobenzene-4,4'-dicarboxylate
3-Bromo-5-iodo-pyridine
BENZALDEHYDE PHENYLHYDRAZONE
Hoechst 33342
Copper(II) acetate
1,9-Diphenyl-1,3,6,8-nonatetraen-5-one
1,3-DibroMo-7-tert-butylpyrene
6-Chloro-5-fluoropyridine-3-carbaldehyde
1-(2-Hydroxyphenyl), 98%
Tris(hydroxyMethyl)nitroMethane, Crystal, Reagent
1-Octanesulfonyl Chloride
methanol-d4
N-methyl-L-glutamic acid
Sulphur yellow 2
Benzyl methyl ether
2,3,5,6-TETRAFLUORO-4-METHYLBENZOIC ACID
METHYL 2,5-DICHLOROBENZOATE
2-(2-Chloroethoxy)-BenzenesulfonaMide
4-(4-Methylphenyl)-3-thioseMicarbazide, 98+%
CycloheptaneMethylaMine, 98+%
2-Ethylhexyl Diphenyl Phosphate
2-AMino-3-broMo-5-fluoropyridine
n-Methyl-2-phenoxy-ethylaMin
N-Methyl 3-broMobenzenesulfonaMide
Diacetin
2-Chlorobenzotrifluoride
2-(1,2-Benzisoxazol-3-yl)acetic acid, 97%
4-Amino-N-methyl-benzenesulfonamide
Methyl 5-aminoisoxazole-4-carboxylate ,95%
Methyl 1-Methylcyclopropane-1-carboxylate
AZINPHOS-METHYL
Pseudotropine
4-NITRO-PYRIDIN-2-YLAMINE
2,4-Dimethyl aniline
Octyl sulfate
2-BroMo-4-(trifluoroMethyl)aniline
D-GlucosaMine OxiMe Hydrochloride
4-AMino-3-nitrobenzenesulfonaMide
EPPS, 99%, for biocheMistry
2-Oxetanone
Butyl ChloroforMate
methyl 5-methoxy-2-nitrobenzoate
cerium(4+) disulphate
dimethyl octafluoroadipate
Nafcillin sodium
Nickel(II) chloride hexahydrate
4-ETHYLPHENOXYACETIC ACID
1,3-BIS[1-(2-HYDROXYETHYL)-4-PIPERIDYL]PROPANE
IRON(II) CHLORIDE N-HYDRATE
Sodium chloride, DNAse, RNAse and Protease free, for molecular biology, 99.85%
4-(4-Ethoxycarbonylphenyl)-3-thiosemicarbazide
2-Aminoethanethiol hydrochloride, 98%
5-Bromo-1,3-thiazole-4-carboxylic acid
1-Boc-(4-benzyl)
Menadione
ALPHA-PINENE OXIDE
Cortisone;17α,21-Dihydroxy-4-pregnene-3,11,20-trione;11-Dehydro-17-hydroxycorticosterone
Methyl triMethylacetate
1-Hexyl-3-MethyliMidazoliuM chloride
DiMethyl PiMeliMidate Dihydrochloride [Cross-linking Agent for Peptides Research]
1-BOC-5-Chloroindole
6-Methylindole, 99%
MERCUROUS CHLORIDE
(5S)-5,6-Dihydro-6,6-diMethyl-5-phenyl-2H-1,4-oxazin-2-one
Holmium(III) oxide
5-(2-fluorophenyl)-1,3,4-thiadiazol-2-amine
1-Chloro-1H-benzotriazole
Titanium boride
NT-3 ELISA Kit
Mouse PINP (Procollagen Type I N-Terminal Propeptide) ELISA Kit
Human TRO ELISA Kit
Human GALNT8 ELISA Kit
Monkey ACE (Angiotensin I Converting Enzyme) ELISA Kit
AD7C.NTP ELISA Kit
VEGF-A ELISA Kit
Human PKCθ (Protein Kinase C Theta )ELISA Kit
Human GPⅡb/Ⅲa (Platelet Membrane Glycoprotein Ⅱb/Ⅲa complex) ELISA Kit
Guinea pig Tumor necrosis factor α,TNF-α ELISA Kit
Violet Red Bile Agar
Lactose Broth
n-Octyl acetate, 98+%
Phenazine, 99+%
4-n-Pentyloxybenzaldehyde, 97%
4-Chloro-2-Iodoaniline
methanol-d4
3,4,5-trichloropyridazine
Basic Red 54
Muskone
Ethyl carbazate
3-(4-Hydroxyphenyl)propionic acid
SodiuM 2-Ethylhexanoate
4-AMino-3-(trifluoroMethoxy)benzoic acid, 98%
Di-n-butyl sulfone, 98+%
1-NaphthylaMine hydrochloride
(S)-4-AMino-2-(tert-butoxycarbonylaMino)butyric Acid
(?)-Myrtenol
2-AMINO-5-BROMOBENZONITRILE
2-BroMo-9,9-diMethylfluorene
4,6-DibroMo-2,3-bipyridine, 95%
3-Methyl-2-cyclohexen-1-one, 98%
(-)-Lupinine, 97%
(R)-(-)-1,2,3,4-Tetrahydro-1-naphthol
2-Tetradecanone