DC2.4细胞:小鼠来源树突状细胞传代要注意什么 货号:GD-C17518419
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞:全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞接收后的操作流程与注意事项:1)如果细胞为贴壁细胞:而收到时呈悬浮或者部分悬浮的状态,请将悬浮的细胞即时离心,加15%血清的完全培养基到新的培养皿/瓶继续培养3天;同时原培养瓶中剩下的贴壁细胞更换为15%血清的完全培养基培养3天。3天后若原瓶或者新瓶中的细胞都没有出现增值而是继续脱落死亡,请及时联系实验室技术人员会跟进解决;2)贴壁细胞生长缓慢;适当提高血清浓度(不能超过20%),或可根据该细胞生长密度,考虑胰酶消化后,转移到新的培养瓶继续培养;3)生长不均:贴壁细胞若出现分布不均,成岛状生长,可将细胞进行消化,重悬打散细胞:加入新鲜培养基进行培养。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
4-(2-Naphthyl)benzeneboronic acid, 97%
Methyl 4-chlorocinnaMate
Ethyl (1R,2S)-cis-2-hydroxycyclohexanecarboxylate
DI-N-NONYLAMINE
D-VALINE METHYL ESTER HYDROCHLORIDE
2,3,6-TRIFLUOROBENZAMIDE
4-BroMo-8-chloroquinoline
5-Bromo-2-chloro-4-methylpyridine
Wood's metal, lump (approx. 100mm)
Arsenic sponge (99.99%)
Acryloxytrimethylsilane inhibited with MEHQ(O-TMS acrylate)
Benzyltripropylammonium chloride
2,2'-Dichlorodiethyl ether
2,6-Dichloro-4-methylnicotinonitrile
1-Chloroisoquinoline
5-BroMo-2-chloronicotinic Acid
N,N'-DiMethyl-3-aMinopyrrolidine
2-BroMo-6-Methylpyrazine
p-Tolyl isocyanate, 99%
Hydrogen Peroxide, 35 Percent Solution, Technical
cis-2-Pentene
1,3,2-benzodioxaborole
dithianon
2,3-dichloropropionic acid
5-Aminosalicylic acid
3-Cyanophenylboronic acid
1,3-BUTADIENE-D6
2-Mercaptoethanesulfonic,Mesna
3-Chloro-5-fluorobenzeneboronic acid pinacol ester, 96%
4-Benzyloxy-6-chloropyriMidine, 95%
2-(TrifluoroMethyl)-4-quinolinol
PotassiuM chroMate
4-CHLOROTHIOANISOLE
TriMethylaMine-15N
2-Chloro-6-(ethoxycarbonyl)pyridine-4-boronic acid, pinacol ester
Cyclooctene , 95%
3-Phenylisoxazole-5-carboxylic acid , 97%
ethyl 2-amino-4-(4-pyridinyl)-3-thiophenecarboxylate(SALTDATA: FREE)
Perfluoro-2,5-dimethyl-3,6-dioxanonanoic acid
Beryllium fluoride 99.5%
Cefminox
5-Bromo-5-nitro-1,3-dioxane
Methyl isonipecotate
DI-2-PYRIDYL THIONOCARBONATE
4-Propylphenylboronic acid (contains varying aMounts of anhydride)
tert-Butyl N-(2-furyl)carbaMate
EthyltriMethylaMMoniuM Iodide
4-ForMyl-2-Methoxyphenylboronic acid
5-Methyl-2-thiophenecarbaldehyde, 97%
2-NITRO-M-XYLENE
5-Methyl-1,3,4-thiadiazole-2-thiol
7-Chloro-1H-indole-3-carboxaldehyde
trans-2,3-dimethoxycinnamic acid
doxepin
D(-)-Erythrose
METHYL 4-BENZYLOXYBENZOATE
9,9-BIS(4-AMINO-3-METHYLPHENYL)FLUORENE
3-BroMo-N,4-diMethylbenzenesulfonaMide
2-Nitrobenzenesulfenyl chloride
4-(Methylsulfonyl)benzyl alcohol
Benzyltriphenylphosphonium iodide, 98%
1,1,1,3,3-Pentafluorobutane
2-Methylanthraquinone
3-Methoxybenzylamine
Janus Green B
Fenbendazole
Testosterone undecanoate
4,4,5,5-TetraMethyl-2-(2-Methyl-3-furyl)-1,3,2-dioxaborolane
(3-BroMopropyl)triMethoxysilane
2,6-Difluoropyridine-4-boronic acid, pinacol ester
Cyclohexylacetylene, 98%
gaMMa-Methyl-alpha-trifluoroMethyl-gaMMa-butyrolactone, 95%
Dodecyl 4-Hydroxybenzoate
Phenyl Vinyl Sulfone
octyl gallate
5-Fluoropyridin-3-ylboronic acid
escin Ia
Calix[8]arene
NAP ELISA Kit
SP-A ELISA Kit
Human TSN ELISA Kit
Human PHOSPHO1 ELISA Kit
Sheep Interleukin 6,IL-6 ELISA Kit
3-NT ELISA Kit
HNRPA1 ELISA Kit
Human S3F ELISA Kit
Human plasmin/fibrinolysin,PL/Fbn ELISA Kit
ANXA2 ELISA Kit
Trypticase Soy-Yeast Extract Broth
Soya Casein Digest Lecithin Polysorbate Agar Base
N-HEPTADECANE
Diglycidyl 1,2-Cyclohexanedicarboxylate
5-Decyn-1-ol, 97%
Methyl 2-hydroxy-1-naphthoate, 98%
1,3,2-benzodioxaborole
2,2',6,6'-Tetramethyl-4,4'-bipyridine
Calcium acetate monohydrate
(±)-2-Amino-1-butanol
DI-N-PROPYL SULFITE
ZEATIN
FMoc-Trp-OH
4-Methyl-1,2,3-thiadiazole-5-carboxylic acid, 98%
2-Iodo-M-xylene, 98%
Neuropeptide Y FragMent 3-36 huMan, rat
N-Benzoyl-L-glutaMic Acid
4-(4-Methylphenyl)benzaldehyde
2′-BroMoacetophenone diMethyl ketal
2-Chloro-3-aMino-4-Methyl pyridine
TRANS-3-HEXENE
Acetonitrile D3 >99.96 (+ 0.03% tms)2 x 0.75ml
3-[(methylamino)carbonyl]benzenesulfonyl chloride(SALTDATA: FREE)
4-(Trifluoromethoxy)aniline hydrochloride , 97%
1-(2-propyn-1-yl)-1H-indole-3-carbaldehyde(SALTDATA: FREE)