ISK细胞系—人子宫内膜癌细胞系全国 货号:GD-C16920533
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞系—将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
(R)-3-AMino-3-(4-cyanophenyl)propionic acid, 95%
Methyl orange
2-Chloro-6-fluoro-3-Methylphenylboronic acid
3-CyanoMethylphenylboronic acid
4-BroMotetraphenylsilane
P-TOLYL CHLOROFORMATE
4-Fluoro-2-methoxypyridine
2-Methoxyisonicotinamide
Methacryloxypropylbis(trimethylsiloxy)methylsilane
3-Piperidin-4-yl-1H-indole, 97%
Hexanal 2,4-Dinitrophenylhydrazone
4-FLUORO-3-AMINO-ACETOPHENONE
TITANIUM (IV) IODIDE
Tetrahydro pyrrole
CalciuM broMide hydrate
Tween? 40
DisodiuM Tiludronate
3-Chloro-1-benzothiophene-2-carbaldehyde
1-Cyclopentene-1-carboxylic acid, 98%
Triclosan, USP
TropyliuM Hexafluorophosphate
N-1,3-dimethylbutyl-N'-phenyl-p-phenylenediamine
1,1-Di-(tert-butylperoxy)-3,3,5-trimethylcyclohexane
Alkali Blue 6B
Nortropine
5-Methylthiazole
1-(4-Fluorophenyl) dihydrochloride
4-AMino-2-broMopyridine
4-Methoxy-1-naphthaldehyde, 99%
LithiuM carbonate, ACS, 99.0% Min
Boc-Ala-OSu
SodiuM acetate
N-Ethyl-o-crotonotoluidide
N-Methyl-1-phenylethanaMine
4-(TRIFLUOROMETHYL)BENZHYDRAZIDE
3-Chloro-4-Methylbenzaldehyde
3-[(2-chloro-6-fluorobenzyl)oxy]benzaldehyde(SALTDATA: FREE)
trans-Di(?acetato)bis[o-(di-o-tolylphosphino)benzyl]dipalladium(II), 97+% [cataCXium?C]
2-Amino-6-methylpyridine, 2-BOC protected 97%
2,3-Difluoro-4-methoxyphenylboronic acid
2-BENZOYLACETANILIDE
3-ACETOXY-2-BUTANONE
Dipotassium hexachloroosmate
1,2-DiMethyliMidazole
Cyclopentyl Methyl ether
DMT-dC(bz) PhosphoraMidite
8-Nonen-1-ol
N-Me-D-Ala-OH HCl
4-Fluoro-3-(trifluoroMethyl)phenacyl broMide, 95%
AMINOACETONITRILE HYDROGEN SULFATE
O-Benzyl-L-tyrosine
4-Bromo-2-furaldehyde
N-tert-butylbenzenesulfinimidoyl chloride
N-(dimethylsilyl)-1,1-dimethylsilylamine
3-(2-Pyridyl)acrylic acid
1-METHYL-1-CYCLOHEXANECARBOXYLIC ACID
2-Fluorophenol
(±)-1-(9-Fluorenyl)ethanol
Hydroquinine-9-phenanthryl ether
3-Cyano-2,6-dichloro-5-fluoropyridine
(2E)-3-(2-Ethoxyphenyl)acrylic acid
1,2-Dichloro-1,2-difluoroethane
trans-4-(Pent-1-yl)cyclohexanecarboxylic acid
3-Aminobenzylmethylamine
1-Piperonyl
METHYL 3,5-DIBROMO-2-HYDROXYBENZOATE
3-Nitrotoluene;Methyl-M-nitrobenzol;M-Ni-trotoluol
N,N,N',N'-TetraMethyl-1,3-propanediaMine
1H-1,2,3-Triazole
N-ForMyl-L-leucine
5-Chlorothiophene-3-carboxylic acid
Methyl 5-chlorosulfonyl-4-Methoxythiophene-3-carboxylate, 97%
SILVEX
2,6-Pyridinedicarboxylic Acid
glycine benzyl ester toluene-4-sulfonate
5-Acetoxymethyl-2-furaldehyde
1,4-Dichloro-2-butanol
5-chloro-1H-indazole
ADM ELISA Kit
LIN28A ELISA Kit
HP-CagA-IgG ELISA Kit
Human Polymorphonuclear Elastase,PMN Elastase ELISA Kit
Monkey Pg (Progesterone) ELISA Kit
IFN-γR ELISA Kit
VDBP ELISA Kit
Human PKCδ1 (Protein Kinase C Delta ) ELISA Kit
Human PAFAH1B3 ELISA Kit
LCN2 ELISA Kit
Peptone Water
Dulbecco’s Phosphate Buffered Saline(D-PBS)
Vinyl decanoate, 95%, stab.
PERPHENAZINE
4-n-Octyloxybenzoic acid, 98%
2-Iodophenol, 98%
N-1,3-dimethylbutyl-N'-phenyl-p-phenylenediamine
Lithium aluminum deuteride
2-Mercapto imidazoline
L(-)-Carnitine
FERULIC ACID METHYL ESTER
Isonicotinic acid
3-Fluoro-4-nitroanisole
Ethyl 3,5-diMethylisoxazole-4-carboxylate, 97%
2,7-Di-tert-butylfluorene, 98%
4-Nitrobenzoic acid
DiMethyl 3,3'-Dithiodipropionate
HYOSCYAMINE SULFATE
Phenoxazine
Lead(IV) fluoride, 99%
(1R,2R)-2-AMINOCYCLOHEXANECARBOXYLIC ACID
Bis(4-tert-butylphenyl)amine
2-Ethoxy-5-methylbenzeneboronic acid 95%
L-tert-Leucine Methylamide
Tetrakis(methylthio)tetrathiafulvalene [Organic Electronic Material]