HP615615细胞系—小鼠肺癌细胞系全国 货号：GD-C16920526
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息，我们拥有丰富的细胞系培养经验，能提供细胞培养全方位的技术支持，让您实验再无烦扰！公司由在国外工作和学习多年的留学人员创办，由国内ZG科学院上海生物化学与细胞生物学研究所，复旦大学，同济大学等多名工作在线的细胞培养专家，提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念，以世界的技术支撑阵容，提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美，提供了重要的基石；公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品，立足国内，面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位，为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内，在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室，以便更好的服务国内广大医院、大学、制药企业等，你们的每一份关心和支持，都是我们前进的动力！
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞系—将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
3-Fluoro-2-(trifluoroMethyl)benzaldehyde, 97%
4-BroMo-2-(trifluoroMethyl)benzaldehyde
AMMoniuM citrate dibasic
Terephthaloyl chloride
1,2,4-Trifluoro-5-nitrobenzene
2-BroMo-N-tert-butylbenzenesulfonaMide
3,4-Dimethoxyphenylacetone
4-(Dimethylamino)-1-(triphenylmethyl)pyridinium Chloride (contains 5% Dichloromethane at maximum)
5-Bromo-N-phenyl-2-pyridinamine
Xylene cyanol FF, pure
Methyl 4-nitro-1H-pyrazole-3-carboxylate
NICOTINE BI-L-(+)-TARTRATE
Didecyldimethylammonium bromide
Adipaldehyde
1,2-Diiodobenzene
2-Chloro-6-Methylisonicotinic acid
4,5-Dichloro-2-n-octyl-4-isothiazolin-3-one
N-Phenyl 2-broMo-5-fluorobenzaMide
2,3-Difluorobenzaldehyde, 98%
TriaMcinolone Diacetate, Micronized, USP
4-Ethynylaniline
sym-Dibenzoylhydrazine
3,3-Dimethyl-1-phenylbutan-2-one
Threne brilliantviolet
2,6-dibromo-4-(chloroimino)cyclohexa-2,5-dienone
3-BROMOBENZALDEHYDE DIETHYL ACETAL
Acetyl ketene
Cobalt (II) SulfaMate
trans-1,2-Cyclohexanediol, 98%
2,2'-Furil dioxiMe, Mixture of isoMers, 97%
3-Fluoro-5-Methylbenzonitrile
4-Fluoro-3-(trifluoroMethyl)benzaldehyde
4-Methyl-5-iMidazoleMethanol hydrochloride
N-Methyl-4-benzenesulfonaMideboronic acid pinacol ester
OCTADECYLAMINE HYDROCHLORIDE
3-NitrophenethylaMine hydrochloride
Trifluoromethanesulfonic anhydride, 98+%
3-Isopropyl-1-methyl-1H-pyrazole-5-carboxylic acid
2-Bromo-5-iodopyridine
3-Bromo-1,1,1-trifluoropropan-2-ol 97%
2-Butene-1,4-diol
N-ALPHA-BENZOYL-L-ARGININE
Oxadixyl
Aminoguanidinium sulphate
AluMiniuM chloride
Boc-D-Valinol
Methyl Propargyl Ether
Methyl 8-Methyl-4-oxo-5-trifluoroMethyl-1,4-dihydroquinoline-2-carboxylate
2-Methyl-L-cysteine hydrochloride, 98%, 98% ee
Tiopronin
L-Cysteine Methyl Ester Hydrochloride
Chromium(III) sulfate hydrate
(S)-N-(2',6'-Dimethylphenyl)Piperidine-2-Carboxylic Amide
Bismuth trichloride
Aniline, 2-(o-tolyloxy)-
Isopropylmalonic acid
2-CHLOROETHYL METHYL SULFIDE
4,4-DIMETHYL-2-VINYL-2-OXAZOLIN-5-ONE
Iron(II) Methoxide
2'-Hydroxy-3',4'-dimethoxyacetophenone
5-Nitro-6-methyluracil ,98%
2-Cyanoethyltrichlorosilane
Potassium formate, pract., 96%
Sulfosalicylic acid
2,3-DIFLUORO-5-(TRIFLUOROMETHYL)PYRIDINE
4-Ethyl-2-methoxyphenol
3,4-Dihydroxybenzaldehyde;3,4-Dihydroxybenzene carbonal;RancinaMycin IV;Protocatechualdehyde
3,4-Dichlorotoluene
1-Ethynyl-4-fluorobenzene
Diphenyl Oxalate
Methyl 3-aMino-2-fluorobenzoate
5-Fluoro-2-Methylphenyl isothiocyanate, 97%
H-PHE-VAL-OH
Methyl 2-BroMobutyrate
4-Benzoylpiperidine hydrochloride
ginsenoside Rd
1,1-Dioxo-tetrahydro-thiopyran-4-one
4-Bromo-m-toluic acid (COOH=1)
Porcine KIM-1 (Kidney Injury Molecule 1) ELISA Kit
CACNα1C ELISA Kit
Hp-IgG ELISA Kit
Human multidrug resistance-associated protein,MRP ELISA Kit
Monkey ApoB100 (Apolipoprotein B100) ELISA Kit
IFNγ ELISA Kit
GC ELISA Kit
PKCg ELISA Kit
Human PAF ELISA Kit
A-FABP ELISA Kit
0.1% Peptone Water Medium
Modified Transport Medium
Vinylboronic acid 2-Methyl-2,4-pentanediol ester, 95%, stab. with phenothiazine
Furan, 99%, stab. with ca 250ppM BHT
5-(1-Pyrrolidinyl)-2-furaldehyde, 98%
2-Iodobenzaldehyde, 98%
sym-Dibenzoylhydrazine
dl-3-(2-naphthyl)-alanine
Phthalocyanine Blue Bsx
Tert-Butyl acetate
CARBAMOYL-DL-ASPARTIC ACID
BARIUM LACTATE
1-(2-Pyridyl)
3-Nitrobiphenyl, 98+%
D-Isosorbide, 98%
4-MethoxycinnaMic acid, predoMinantly trans
Difenzoquat
3-(2-Quinolyl)-DL-alanine
Ethyl pivaloylacetate
2-Chloronicotinyl chloride
DL-Isoleucine
1-(Trimethylsilyl)-1H-benzotriazole
L-Aspartic acid diallyl ester p-toluenesulfonatesalt
4-Nitrophenyl trifluoromethyl sulphoxide
1,4,5-trimethyl-1H-imidazole-2-carbaldehyde(SALTDATA: FREE)
