LN-229细胞系—人大脑神经母瘤细胞系全国 货号:GD-C16920076
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
接收细胞相关问题:如不能在收到细胞后及时操作细胞系—T25及离心管发货的细胞将可以消毒后在37度过夜,血清发货的细胞在室温下静置1-2天(注意不要放冰箱),干冰发货的可以在确认干冰未完全升华时将细胞直接放回液氮或者-80度冰箱,若干冰完全升华,请即刻复苏细胞。T25瓶及离心管在培养箱平衡是为了让细胞尽快适应培养箱环境,同时使部分因运输导致脱落的细胞贴壁,此步骤非常必要。T25瓶如果在显微镜下可以看到部分不贴壁的细胞系—可以收集上清后离心(1200rpm,5min)后,将沉淀用新的培养基重悬后接种至新的培养瓶或皿中。部分细胞在运输过程中,由于不断震荡及环境不适,可能导致细胞破碎死亡,从而使培养基中漂浮很多细胞碎片及颗粒状物质。这种情况下,平衡后,贴壁细胞用PBS洗两遍再加新的培养基培养或者传代至新瓶中培养即可;悬浮细胞可以离心(1000rpm,3min)收集细胞系—并用PBS重悬后再离心一次,再用新的培养基重悬并接种细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2-BroMophenol, 98%
TetraphenylarsoniuM(V) chloride hydrate
3-HYDROXYBUTYRIC ACID
TREHALOSE
LutetiuM(III) acetate hydrate, REacton(R), 99.9% (REO)
DIMETHYL 3,4-DIHYDROXYPYRROLE-2,5-DICARBOXYLATE
2,4-DIAMINOBENZOIC ACID DIHYDROCHLORIDE
Iron(II) sulfate heptahydrate, 99+% (ACS)
1-Dodecene [Standard Material]
2,6-Dichloronitrobenzene
2,3,4,5,6,6-Hexachloro-2,4-cyclohexadien-1-one, 99%
YTTRIUM CHLORIDE HEXAHYDRATE
METHYL 5-METHOXYSALICYLATE
3,4-Dinitrochlorobenzene
4-Chloro-3-methylaniline
D(-)-Quinic acid
N,N-Diethyl-2,3-diMethylbenzaMide
1,2-DibroMo-3,5-difluorobenzene
ThyMol, 99%
PalMitoleic Acid
DiMethyl Hexadecanedioate
1,2-Dimethylcyclohexane
3-Hydroxymethylpyrrolidine
1-Bromoadamantane
meso-Erythritol
1,1,3-TRIMETHOXYPROPANE
2-Oxo-1-imidazolidinecarbonyl chloride
4-BroMo-2-Fluoroacetophenone
3-EthylaMino-4-Methylphenol, tech. 90%
2-(TrifluoroMethyl)pyridine-5-carboxaldehyde, 95%
N-FMoc-3-(1-naphthyl)-L-alanine, 98%
9-BroMophenanthrene
(S)-1-[(RP)-2-(Diphenylphosphino)ferrocenyl]ethyldi-tert-butylphosphine
SACCHARIN SODIUM SALT DIHYDRATE
N-LAURYLDIETHANOLAMINE
5-BroMo-2,3-difluorophenol
1-ACETYL-3-THIOSEMICARBAZIDE
2'-Aminoacetophenone
Dibutyldichlorosilane
N-Boc-3-Carboethoxy-4-Piperidone
Bis(4-nitrobenzyl) Malonate
5-DIMETHYLAMINO-2-METHYL-3-PENTYN-2-OL
POTASSIUM THIOSULFATE
BARIUM HYDRIDE
4-Piperidinobenzaldehyde
N,N-DiMethyl-N-[2-(2-pyridyl)ethyl]aMine
N-(1,3-DiMethylbutyl)-N'-phenyl-1,4-phenylenediaMine
Ethyl 8-broMo-6-chloroiMidazo[1,2-a]pyridine-2-carboxylate
Tricosane, 98%
PotassiuM Ferricyanide, Reagent, ACS
Di-p-tolyl Disulfide
Pentabromophenol
Tetrabromoterephthalicacid; 98%
4-Pyridinecarboxaldehyde
4-Nitrophenyl dihydrogen phosphate
N,N'-Diphenylbenzidine
ALPHA,ALPHA,4-TRIBROMOACETOPHENONE
4,6-Dichloro-5-MethoxypyriMidine
Flavin adenine dinucleotide disodiuM salt
(4-methoxybenzyl)hydrazine(SALTDATA: HCl)
2-(Thien-2-yl)benzoyl chloride 97%
5-Hydroxy-4-methoxy-2-nitro-benzaldehyde
Fluoral-P (=4-Amino-3-penten-2-one) [Fluorimetric reagent for aldehydes.]
1,1,2,2-Tetrafluoroethyl-2,2,3,3-tetrafluoropropylether
N,N-Dipropyl-1-propanamine
Mucochloric acid
4-Bromo-2-methylbenzoic acid
3-Fluoroisonicotinic acid
1,3-Bis(benzyloxy)-2-propanol
5-Chloro-2-isobutoxypyridine-3-boronic acid
Bis(pinacolato)diboron, 98%
2-BroMoMethyl-1,3-dioxolane, 97%
N-Methylbis(trifluoroacetaMide) [Trifluoroacylating Agent]
4'-Chloro-3-hydroxy-2-naphthanilide
1,3,5-tris(trifluoromethyl)benzene
4-Hydroxy-2-methyl-5-nitrobenzoicacid
2'-Hydroxyacetophenone
1-methyl-1H-pyrazol-4-amine
BRAK ELISA Kit
TUBB ELISA Kit
LPL ELISA Kit
Human CPLX1 ELISA Kit
PGE2 ELISA Kit
KISS1R ELISA Kit
VEGFR-3/Flt-4 ELISA Kit
Human ADCYAP1 ELISA Kit
FAM160a1 ELISA Kit
VE ELISA Kit
Meclary Agar
Free Biotin Assay Medium
Ginkgo biloba extract
ChroMiuM
5-AMino-2-BroMopyridine
2-Methyl-1-butanethiol
1,2-Dimethylcyclohexane
magnesium thiosulfate hexahydrate
DL-2-Phenylglycinol
zinc di(benzothiazol-2-yl) disulphide
2-Fluorobenzoyl chloride
2-BroMophenol
4-Chlorotoluene, 98%
7-AMino-4-MethylcouMarin, 98%
Iodoethane, 98+%, stab. with copper
2-Methoxybenzoic acid
4-Chloro-3-nitroaniline
Polyethylene glycol MonoMethyl ether Mesylate
BUTOPYRONOXYL
2-AMino-3-broMo-5-nitropyridine
3-(4-BroMophenyl)-6-Methyl-2H-1,4-benzoxazine
N-BOC-Amino-(4-N-FMOC-piperidinyl)carboxylic acid, 95%
1,3-Dichlorobenzene
3-Amino-5-benzyl-4H-1,2,4-triazole
Palladium(II) sulfate dihydrate