HT29细胞系—人结直肠腺癌细胞系全国

HT29细胞系—人结直肠腺癌细胞系全国 货号：GD-C16919253

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息，我们拥有丰富的细胞系培养经验，能提供细胞培养全方位的技术支持，让您实验再无烦扰！公司由在国外工作和学习多年的留学人员创办，由国内ZG科学院上海生物化学与细胞生物学研究所，复旦大学，同济大学等多名工作在线的细胞培养专家，提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念，以世界的技术支撑阵容，提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美，提供了重要的基石；公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品，立足国内，面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位，为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内，在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室，以便更好的服务国内广大医院、大学、制药企业等，你们的每一份关心和支持，都是我们前进的动力！
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞系—其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Triethyltin chloride
Substance P FragMent 1-9
Methyl 4-chloro-6-fluoroquinoline-2-carboxylate
2,5-DibroMoselenophene
OctadecanaMine
2-BroMoethyl Benzoate , 98%
COPPER VANADATE
2,4-Dichloro-5-methylbenzotrifluoride
2-(acetyloxy)-5-chlorobenzoic acid(SALTDATA: FREE)
3-Chloro-6-methyl-benzo[b]thiophene-2-carboxylicacid
2-(Trifluoromethyl)pyrimidin-4-amine ,97%
TYRAMINE HYDROCHLORIDE
1,4-Phenylenediacetonitrile
Cyclohexyl chloroformate
2-AMino-1-butanol
4-Ethylaniline
1,4-DibroMo-2,5-diiodobenzene
2-BroMo-5-(diethoxyMethyl)-3-Methylthiophene
Zinc(II) acetylacetonate, ca. 25% Zn
Sucralose, NF
2,4,6-Trichlorobenzenesulfonyl Chloride
1,2-Phthalic dicarboxaldehyde
2-methylpentanenitrile
N-Benzyloxycarbonyl-L-glutamic acid
4-fluorobenzyl alcohol
1-N-Boc-Aminocyclopentanecarboxylic acid
tert-Butylhydroquinone
2'-MethoxycinnaMaldehyde
5-Acetylsalicylic acid, 98+%
n-Pentylcyclohexane, 98%
Stannic chloride
Triethyl borate
HEXAMETHYLBENZENE
OctafluoroadipaMide
2-AMinooctane , 98%
BISPHENOL A BIS(CHLOROFORMATE)
N-Boc-cyclopropyl alanine
Dichloro(1,5-cyclooctadiene)ruthenium(II) polymer, min. 97%
Luzindole
Acid Black 48, pure, 98%
Whiskey lactone
Carbenoxolone disodium
5-Phenylvaleric acid
(-)-Camphanic acid chloride
p-Toluquinone
Naphthol Green B
1-(ForMaMidoMethyl)-1H-benzotriazole
(R)-3-AMino-3-(3-chloro-phenyl)-propionic acid
3-(MaleiMidoMethyl)-PROXYL, 98%, free radical
5-AMINO-1H-TETRAZOLE
1-AMino-2-Methylanthraquinone
2,6-Diaminopurine
virginiamycin
Dodecanenitrile
Pentafluorobenzonitrile
(2'-METHOXY-BIPHENYL-2-YLMETHYL)-METHYL-AMINE
2-FLUORO-5-IODOBENZOYL CHLORIDE
3-(4-(2-Hydroxyethyl)-1-carbonyl)phenylboronic acid, HCl
2,5-BIS(TRIFLUOROMETHYL)PHENYLACETONITRILE
Ethyl 2-chloro-6-methylpyrimidine-4-carboxylate , 97%
Bis(2,4,5-trichlorophenyl) Disulfide
trans-4-methycyclohexyl isocyanate
6-Iodo-2,3-dihydro-1,4-benzodioxine , 97%
2,6-Dimethoxybenzaldehyde
4-N-BUTOXYPHENYLACETIC ACID
Clostebol
Vincristine Sulfate
Ethyl 2-broMobutyrate
1-BroMo-4-Methylpentane
Methyl 1,3-benzodioxole-5-carboxylate
8-AMinoquinoline, 98+%
tert-Butyl 4-[4-(hydroxyMethyl)benzyl]tetrahydro-1(2H)-pyrazinecarboxylate, 95%
Diethyl 2,2,3,3,4,4-Hexafluoropentanedioate
2,4,6-Trifluorobenzoic Acid
Alliin
1-Butyl-3-(1-naphthoyl)indole
4-Methyl-3-(trifluoromethyl)bromobenzene
Reactive Brill. Blue K-GR
FLNB ELISA Kit
S100 ELISA Kit
Human PCDHβ11 ELISA Kit
HrBP ELISA Kit
Chicken N-acetyl-β-D-glucosaminidase,NAG ELISA Kit
β2-MG ELISA Kit
Chicken Anti-Thrombin Ⅲ,AT-Ⅲ ELISA Kit
Human CA ELISA Kit
Human SGK2 ELISA Kit
HBeAg ELISA Kit
Modified Y Medium
PAC Slant Medium
Diethyl acetylsuccinate, 97+%
PraseodyMiuM(III) fluoride, anhydrous, 99.9% (Metals basis)
3-Carboxy-4-Nitrophenyl Disulfide
3-BroMo-2-Fluoropyridine
1,2-Phthalic dicarboxaldehyde
Octachlorotrisilane
vitamin K2
1-Chloro-3-methyl-2-butene

2-(4-Chlorobenzoyl)benzoic acid
2,6-Dichloronicotinic acid
Boc-Tyr-OH
2,3,4,5,6-Pentafluorobenzeneboronic acid, 97%
2-(1-NaphthylMethyl)-2-iMidazoline hydrochloride, 99%
1-(4-BroMophenyl)naphthalene
Tetramethylammonium hydroxide
2,6-DiMethoxypyridine-4-boronic acid, pinacol ester
2-Methoxyethanol
(2-CHLORO-5-TRIFLUOROMETHYL)PHENYLTHIOUREA
2,3,4-Trifluorophenylboronic acid
4,6-Dichloro-o-cresol
Sulfamic acid ,99.5% [for analysis]
n-Octyldimethylmethoxysilane
Octyl 4-O-(a-D-glucopyranosyl)-b-D-glucopyranoside ,98%