NCTC1469[NCTC1469]细胞系—小鼠正常肝细胞系全国 货号:GD-C16919201
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞系—其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2-Picolinic acid, 99%
2,4,5-TriMethoxybenzoic acid
2,4-DiMethoxyacetophenone
Methyl ethyl(tributylstannyl)carbaMate
Methyl TriphenylMethyl Ether
4-Butoxy-2,3,5,6-tetrafluorophenylboronic acid
2-BROMO-5-IODO-3-METHYLPYRIDINE
Tetraallyltin
(R)-2-(Aminoethyl)-1-N-Boc-pyrrolidine
3-(Cyclohexylamino)-2-hydroxy-1-propanesulfonic acid
1-Boc-3-oxoazepane
3-BROMO-2,4-DICHLOROTOLUENE
BENZYL TERT-BUTYL MALONATE
(R)-(+)-4-Isopropyl-2-oxazolidinone
2-Chloroethyl isothiocyanate
Perfluorobutyl iodide
2,2-DiMethyl-5-(3-thienyl)-1,3-dioxane-4,6-dione
N-Methyl 3-broMobenzaMide
Vanadyl(IV) acetylacetonate, 99%
SodiuM Propionate, FCC
3-FluorocinnaMic Acid
4-Methoxystyrene
4-Methoxy-3-hydroxyacetophenone
3-Methyl-4-nitropyridine-1-oxide
1-fluoro-4-(methylsulphonyl)benzene
6-Chlorohexanol
2,4-DIETHOXY-BENZALDEHYDE
3-Chlorobenzyl Alcohol
Zinc 2-Methoxyethoxide, 5% w/v in 2-Methoxyethanol
CyanaMide, 50% w/w aq. soln., stab.
(R)-(-)-3-Chloro-1,2-propanediol
Hypericin
4-Methoxy-3-(trifluoroMethyl)benzoyl chloride, 97%
PENTADECANOIC ACID
4,6-Dichloro-2-MethylpyriMidine
4-BroMo-2-nitroaniline
2-Methylpyrazine
1-(2-Bromoethoxy)-3-chlorobenzene, 95%
2-(5-Benzyl-1,3,4-oxadiazol-2-yl)ethanamine
3-Aminopropane-1,2-diol 99%
Pentafluorophenol
Solifenacin succinate
3-Amino-2-methylbenzoic acid
gamma-Valerolactone
2-Chloro-3,5-dibroMo-6-Methylpyridine
4-{[1-(tert-Butoxycarbonyl)-4-piperidinyl]oxy}benzoic acid
beta-Estradiol 17-Cypionate
4-[(3-Chlorophenyl)carbonyl]benzonitrile
1,2-DibroMobutane, 97%
METHYL SULFIDE
4-tert-Butylphenylboronic Acid (contains varying aMounts of Anhydride)
4-Chlorobenzenediazonium hexafluorophosphate
Calcium Phosphate Tribasic
(1R)-endo-(+)-Fenchol
2,4,5-Trichloroaniline
(R,R)-2,3-Butanediol
1-Diazo-2-naphthol-4-sulfonic acid
Trichloroacetic acid
3-BroMo-6-chloroiMidazo[1,2-b]pyridazine
4'-N-Piperidinophenyl acetylene
p-Tolymethyldichlorosilane
Copper(I) trifluoromethanesulfonate benzene complex ,88%
2-Amino-3-(dimethylamino)pyrazine
2-CHLORO-6-FLUORO-3-METHYLBENZOIC ACID
1-Bromo-4-iodobenzene
1,2-BENZANTHRAQUINONE
Lysozyme hydrochloride
Uridine 5'-Monophosphate
1-BroMo-2,3,4-trifluorobenzene
Methyl alpha-L-Fucopyranoside
5,6,7,8-Tetrahydro-iMidazo[1,2-a]pyridine-2-carboxylic acid
5-BroMo-2-nitrobenzotrifluoride, 98%
Atropine
3-Ethyl-3-oxetaneMethanol
P-TOLUENESULFONYL SEMICARBAZIDE
2-Benzyl phenol
poly(ethylene glycol) monolaurate
3-Phenylglutaric acid
CA2 ELISA Kit
Mouse small nuclear ribonucleoprotein,snRNP/Sm ELISA Kit
Human progestin and adipoQ receptor family member Ⅶ,PAQR7 ELISA Kit
Human E2/ubiquitin-conjugating enzyme,E2/UBCE ELISA Kit
Chicken IL-13 (Interleukin 13) ELISA Kit
Rat Interferon α,IFN-α ELISA Kit
Ghrelin ELISA Kit
Human cholesterol,CH ELISA Kit
Human HBμ ELISA Kit
NOS ELISA Kit
Nutrient Agar, pH6.0
Soya Casein Digest Lecithin Polysorbate Agar Base
2-Ethoxy-Malonic Acid Diethyl Ester
Fluconazole
5,7-Dichloro-8-hydroxyquinoline, 99%
2-Fluoro-4-Nitrobenzonitrile
4-Methoxystyrene
2,5-Diethylpyrazine
1-Bromo-5-Hexanone
Nipecotic acid
Polysucrose
2-Amino-3-nitropyridine
L-Carnitine-L-tartrate
4,4'-Diisopropylbiphenyl, 97%
3-Ethoxybenzoyl chloride, 98%
Phenylhydrazine-4-sulfonic Acid
Sulfobetaine 8
DIGLYCEROL
1-Methylpyrrolidine
Tilorone dihydrochloride
4-N-PENTYLBENZOIC ACID 4'-N-BUTOXYPHENYL ESTER
1-(4-Methylphenyl)-1-cyclopropanecarboxylic acid, 98%
1-Benzo[b]thiophen-3-yl-2-bromoethan-1-one, 97%
Lithium chromate, min. 95%
Guanylurea Phosphate