PC-2细胞系—人癌细胞系全国减价 货号:GD-C16819714
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
养了几年细胞系—说一点自己培养细胞方面的看法:1)培养细胞前,可以看看细胞特点说明书,包括细胞正常生长的状态图、传代、培养基的类型等;2)不同细胞生长周期不同,有的生长很快,比如说MDA-MB-231,传代的时候取离心悬液的十分之一就已经足够了,有的又是特别慢,等的人心焦,BT474就是,传代是一分二,复苏起始的时候两周多才能长满,所以就要在培养细胞的过程中多多摸索了;3)对于娇弱的细胞系—就得细心呵护了,胰酶消化不能过久,吹得时候要轻柔,离心时候也要注意转速和时间,每天都要去看一下细胞系—肉眼观察培养基状况、显微镜下观察细胞生长状况;4)冻存细胞复苏后第二天Z好更换一次培养基;5)培养箱隔一段时间彻底用酒精棉擦洗一次;6)养细胞的教训:不能偷懒!细胞虐我千百遍,我待细胞如初恋。1)不管买的细胞、惠赠的细胞系—刚拿到手赶紧做两件事:检测是否有支原体污染; 迅速扩增冻存;2)发现有污染迅速处理掉,特别是当你养了很多种细胞时; 细菌污染,真菌污染很容易发现,支原体污染的话,细胞会长的慢,买个支原体检测的kit定期检测一下;3)传代细胞日常的值日清洁是必须的,此外还要定期熏蒸;4)细胞的传代一定要规律,保持相同的confluency和传代时间间隔,不要随心所欲或者拖延;5)注意个人卫生。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
4-Chloro-2-Methyl-6-nitroaniline
Cyclohexanone oxiMe
D-GlucosaMine hydrochloride
1,2-Diethoxybenzene
DiMethyl 5-hydroxyisophthalate
4-Chloro-6-MethylaMinopyriMidine
3-(Trifluoromethoxy)phenylacetonitrile
4-(Aminomethyl)chroman-4-ol
2-(Methylamino)-3-nitropyridine
Methyl Tridecanoate
2-ACETYL-5-IODOTHIOPHENE
1-PHENYL-5-(TRIFLUOROMETHYL)PYRAZOLE-4-CARBONYL CHLORIDE
Strontium peroxide
Methyltriacetoxysilane
3-Furylboronic acid (contains varying aMounts of Anhydride)
6-(BroMoMethyl)-1,1,4,4-tetraMethyl-1,2,3,4-tetrahydronaphthalene
9-Ethylcarbazole-3-carboxaldehyde N-Ethyl-N-(M-tolyl)hydrazone
5-Chloro-2-hydroxypyridine-3-carbonitrile
(R)-BOC-2,4-Dichlorophenylalanine
DI-N-OCTYL PHTHALATE
1H,1H,2H-Heptadecafluoro-1-decene
2-Phenylcyclohexanone
glucose-6-phosphate dehydrogenase
3,5-diphenyl-4H-1,2,4-triazole
4,4'-Dimethyldiphenylamine
3,5-DI-TERT-BUTYL-4-HYDROXYACETOPHENONE
2,3,4-Trifluorobromobenzene
Pseudotropine
(R)-2-Methylpyrrolidine p-toluenesulfonate, 97%
5-Chlorosalicylaldehyde, 98%
PCB No 18
Trigonox® 101
Cellulose PAB Capacity: 0.20Meq/g
N-(tert-Butoxycarbonyl)-3-broMoaniline
Methyl 3,4,5-Tris(benzyloxy)benzoate , 98%
3,4-(Methylenedioxy)phenyl isocyanate
1,4-Bis(2-hydroxyhexafluoroisopropyl)benzene 97%
Lanthanum(III) chloride hydrate (99.999+%-La) (low Ca, Fe, Mg) PURATREM
3-(Trifluoromethoxy)iodobenzene 98%
2-(4-fluorophenoxy)ethanamine(SALTDATA: CH3SO3H)
Chlorfenazole
Pyruvic acid
1-CYANOACETYLPIPERIDINE
Methylthioethane
EthylMagnesiuM chloride
Vinylene carbonate
O-TBDPS-D-Thr-N-Boc-L-tert-Leu-Diphenylphosphine
3,3-DiMethyl-1-butyne, 98%
(1S,2R)-(+)-2-BenzaMidocyclohexane carboxylic acid, 98%
Tetracycline
Phthalic Hydrazide
methylmagnesium bromide
Quinclorac
4-AMINO-3-CHLORO-5-NITROBENZOTRIFLUORIDE
ethyl 3,3-diethoxyacrylate
Methyl 3-amino-4-methylthiophene-2-carboxylate
3-AMinopyrrolidine dihydrochloride
TRANS-4-AMINOCYCLOHEXANECARBOXYLIC ACID
γ-Oxo-1-pyrenebutyric acid
4-(2-chloroethyl)-1H-pyrazole(SALTDATA: HCl)
Trimethyl Orthovalerate
1,5-Isoquinolinediol, 98%
Tetrabenzyl Pyrophosphate
3-Nitro-5-(trifluoromethyl)phenol
N-(4-Chlorophenyl)-2-chloroacetamide
3-AMINO-2-BROMO-5-CHLOROPYRIDINE
Vinyl acetate
2-IMidazolidinethione
2,3,5-DCTF
5-Nitroso-8-hydroxyquinoline
4-Methoxyphenyl Mesylate
2,4-Difluorobenzene-1-thiol, Tech.
D-Tyrosinol hydrochloride
3-(4-Nitrophenyl)propionic Acid
1-bromo-3,5-dimethoxybenzene
Dihydroxyaluminium aminoacetate
3-Bromo-2-furoic acid
Acid Red 374
BDNF ELISA Kit
COL7 ELISA Kit
Human IGF1R3 (Insulin Like Growth Factor 1 Receptor 3) ELISA Kit
Human ConA ELISA Kit
ILK-1 ELISA Kit
IL-13 ELISA Kit
TGFβ1 ELISA Kit
DPA ELISA Kit
Human IGF2R ELISA Kit
COL7 ELISA Kit
Folic Acid Assay Medium
Chapman Stone Agar Medium
Zinc DisodiuM Edta
1,2-CYCLOHEXANEDICARBOXYLIC ACID
4-Ethylphenylzinc iodide, 0.5M in THF, packaged under Argon in resealable CheMSeal^t bottles
2-AMino-6-broMobenzonitrile, 95%
2-Phenylcyclohexanone
1-(4-aminoindoline)ethanone
(S)-(+)-4-Methyl-2-pentanol
Cholesteryl propionate
Ethyl benzoylacetate
Cupric Acetylacetonate
Methyl 3-broMo-4-Methylbenzoate, 99%
D-GlutaMic acid 1-tert-butyl ester, 98%
LithiuM rod, 12.7MM (0.5in) dia x approxiMately 165MM (6.50in) long, 99.9% (Metals basis)
Xylitol
6-BroMoindole-3-carboxaldehyde
2-Butyl-6-phenylpyridine
Pyridine-2-carboxaldehyde 4-nitrophenylhydrazone, 98%
1-(3-BroMophenyl)-3,5-diMethylpyrazole
3-METHYL-4-PHENYLPYRAZOLE
2-[2-(2-Azidoethoxy)ethoxy]ethyl 2,3,4,6-Tetra-O-acetyl-D-galactopyranoside
3,5-Dimethoxyphenyl isothiocyanate
4-(Difluoromethylthio)aniline 98%
(2R)-(+)-3,3'-Diphenyl-[2,2'-binaphthalene]-1,1'-diol, min. 98% (R)-VANOL