NCL-H548细胞系—人癌细胞系全国减价 货号:GD-C16819703
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难,Z关键几点:1)所有的东西使用Z好的,如果你用的血清、培养皿、培养基、胰酶什么的都是进口的,真的很难相信你会把细胞养坏;2)动作要快,胰酶消化,换液什么,细胞暴露在空气中的时间越少越好。另外,要掌握好胰酶消化时间,所谓的细胞状态差,很多时候是传代的原因;3)有时间的话,需要细胞计数,传相应数目的细胞到培养皿中,可以大致清楚细胞的生长曲线,不会临时要处理,手足无措;4)要做什么心里要非常清楚,合理安排时间,传代细胞的实验穿插进行,可以节省很多时间,也不会耽误别人的实验;5)个人的实验器具自己收一套,不要和别人混用,Z近换了什么新的东西稍微记一下,试剂一旦怀疑污染,马上丢;6)传代细胞不能进去太多人,避免相互干扰。每天对待细胞比孝敬爹妈还用心,那真是捧在手里怕碎了,含在嘴里怕化了,看在眼里怕丢了,培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作,多喷喷酒精,要是对灭菌的东西不放心,自己包自己送自己烘干;2)不要和别人共用试剂耗材,自己准备一套;3)有可能的话在拿到新细胞的时候做好支原体衣原体检测;4)水浴锅很脏,生化培养箱要是得手动加湿的话盘里的水也会很脏,勤换(灭菌水加进去);5)晚上离开的时候Z好给传代细胞照紫外,超净台是用完就开;6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
BroMoacetaldehyde diMethyl acetal
Chlorocyclobutane
(2-METHYLPHENOXY)ACETIC ACID
(AMinoMethyl)cyclohexane
Chloro(2-dicyclohexylphosphino-2′,4′,6′-triisopropyl-1,1′-biphenyl)[2-(2-aMinoethyl)phenyl)]palladiuM(II)
BETA-BUTYROLACTONE
Phenoxyacetic acid, 98+%
4-Methoxyphenyl 3-O-Benzyl-beta-D-glucopyranoside
4-Fluoro-3-nitrobenzoyl chloride
2-Fluoro-6-(trifluoromethyl)benzyl alcohol
2-Fluoro-4-methylphenylboronic acid
3,4-Dimethylbenzylamine
sec-Butylamine
2-BROMO-4-FLUOROCINNAMIC ACID
4-BroMo-2-thiophenecarbonyl chloride
(2-Pyrrolidin-1-ylpyrid-3-yl)MethylaMine
4-Ethynylpyridine
2-AcetaMido-5-nitrophenylboronic acid, pinacol ester
4-(Piperidin-1-ylMethyl)aniline, 97%
FREON 12
2,2':6',2''-Terpyridine
Vinylpentamethyldisiloxane
Alpha Amylase
3,4-Dimethoxyphenol
m-Tolualdehyde
2,6-Dimethoxybenzoic acid
8-Hydroxyquinoline aluminum salt
Cholesterol
1,4-Butanesultone, 99%
4-DiMethylaMinoazobenzene-4'-sulfonyl chloride, 98+%
2'-Chloro-5'-(trifluoroMethyl)acetophenone
4-(DiMethylaMino)phenylboronic acid
1,4-Diacetoxy-2-broMobenzene
N,N,2-TriMethylaniline-4-boronic acid
2-BROMO-5-CHLORO-3-METHYLBENZO[B]THIOPHENE
3-(Benzyloxy)-5-Methylphenylboronic acid
2-Methyl-2,3,4,5-tetrahydro-1H-pyrido[4,3-b]indole
2-[(3,4-dimethyl-2-oxo-2H-chromen-7-yl)oxy]propanoic acid(SALTDATA: FREE)
3-(Trifluoromethyl)phenethyl alcohol, 97%
(S)-Tetrahydro-1,3,3-triphenyl-1H,3H-pyrrolo[1,2-c][1,3,2]oxaborole, 99% (S)-Phenyl oxazaborolidine
Diniconazole
(R)-(-)-Methyl glycidyl ether
4-METHYLNONANOIC ACID
4-BroMotoluene;4-BroMo-1-Methylbenzene;p-Tolyl broMide
1,5-DiMethyl-1H-pyrazole-4-boronic acid,pinacol ester
3-(2-Furyl)acrolein
Dichloro(Methyl)octadecylsilane
7-Chloro-2,8-diMethyl-4-hydroxyquinoline
(+)-N-Tosyl-L-glutaMic acid, 98%
4-Phenylmorpholine
4-ForMylphenoxyacetic Acid
3-bromo-4-methylthiophene
5-Chloro-6-hydroxynicotinic Acid
(S)-(-)-2-Chloro-3-[4(5)-imidazolyl]propionic Acid
malt
2-Bromonaphthalene
trans-3-(TrifluoroMethyl)cinnaMoyl chloride
Triisobutylphosphine sulfide
3-Phenylpropionic acid Methyl ester
5-Amino-1-phenyl-1H-pyrazole-4-carbonitrile
4-Fluorobiphenyl
Hydrocortisone
N-(2-fluorophenyl)glycine(SALTDATA: FREE)
Formononetin
3-Nitrovalerophenone
4-Cyanobenzyl bromide
Diastase;Diastase of Malt;Maltin
4-Fluoro-3-nitrobenzaldehyde
2,3,4,6-Tetra-O-acetyl-α-D-glucopyranosyl fluoride
4-Isothiocyanato-2,2,6,6-tetraMethylpiperidine 1-Oxyl Free Radical
2,6-DichloroiMidazo[1,2-b]pyridazine
Ethyl sorbate, 97%, stabilized
2,7-Diaminofluorene
6-Benzyloxyindole
Glycinonitrile hydrochloride
2-Cyanopyrazine
L(+)-Glutamic acid monosodium salt monohydrate
2-methylbutyl 2-methylbutyrate
ET-1 ELISA Kit
Mouse AgRP (Agouti Related Protein) ELISA Kit
Human IGFBP-1 ELISA Kit
Human dengue fever antibody,DF ELISA Kit
FABP ELISA Kit
CD97 ELISA Kit
PDGF-BB ELISA Kit
PON ELISA Kit
Human IGF-1R ELISA Kit
PⅢNP ELISA Kit
Maltose yeast extract agar (Okazaki and Okami, 197
Acetamide Agar
Glyoxalbis(2-hydroxyanil)
trans-1-Fluoro-4-(4-n-heptylcyclohexyl)benzene, 97%
4-Ethylbenzoyl chloride
2-AMinobenzyl alcohol
Vinylpentamethyldisiloxane
3-Chlorothiophene
N-Ethylbenzylamine
2,6-Dimethylbenzoic acid
4-(TRIFLUOROACETYL)BENZOIC ACID
4-(2-Aminoethyl)benzenesulfonylfluoride hydrochloride
Guanidine Nitrate
3-BroMo-5-fluorobenzoic acid, 97%
4,5-DiMethylbenzene-1,2-diMethanol, 98%
1-Boc-1,2,3,6-tetrahydropyridine
TOTU
SILVER THIOCYANATE
Toluene-4-sulfonic acid dibenzyl-α-ylidenehydrazone
(S)-(+)-1-Cyclohexylethyl isothiocyanate, 97%
DL-ALPHA-AMINOPIMELIC ACID
1H-Benzimidazole-2-thiol 97%
Diisopropyl azodicarboxylate ,98%
6-Methoxy-4-methylquinoline
3-iso-Propyl-1-methyl-1H-pyrazol-5-amine