HUVECs细胞系—人脐静脉内皮细胞系全国 货号:GD-C16918901
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结:1)培养基中血清的比例多少合适?血清是细胞培养上一个极为重要的营养来源,所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%,不要低于5%或高过20%,因为含量过低会导致细胞营养不足,过高则会破坏渗透压平衡。一般建议血清含量为10%,可以适量加减;2)何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上的更换时间,按时更换培养基即可。还可以参照指示剂的颜色变化进行更换;3)购买的复苏细胞系—为何会有脱落?因为运输的过程中不可避免的会有震荡,收到后可以离心收集;4)购买的细胞冷冻管经解冻后,为何会发生细胞数目太少之情形?研究人员在冷冻细胞之培养时出现细胞数目太少,大都是因为离心过程操作上的失误,造成细胞的物理性损伤,以及细胞流失。建议细胞解冻后不要立刻离心,应待细胞生长隔夜后再更换培养基即可;5)购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,常见原因可归纳为:培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后,加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久;6)收到的冷冻管瓶身破裂,瓶盖有裂纹,或瓶盖脱落之原因?冷冻管瓶盖裂纹,或瓶身破裂,可能是因为操作者夹取冷冻管时用力不当,造成冷冻管裂损,建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱,乃因热胀冷缩之物理现象,冷冻管有可能因此而造成细胞污染,故冷冻管于放入和取出液氮桶时,均应立刻将冷冻管再一次扭紧。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2-BroMotoluene, 99%
FMoc-Lys(Ddiv)-OH
METHYLDICHLOROPHOSPHINE
3-METHYLFORMANILIDE
2-BroMo-4-fluorophenol
3-(TrifluoroMethyl)benzhydrol, 97%
5-BroMo-4-(3,4-diMethylphenyl)pyriMidine
2,4,6-Tribromophenyl Hexanoate
2-Amino-3-methyl-5-nitropyridine
4-Butyl-2-methylphenyl isocyanate, tech
Dimethylammonium dichlorotri(?chloro)bis{(S)-(-)-2,2'-bis[di(3,5-xylyl)phosphino]-1,1'-binaphthyl}diruthenate(II) [NH2Me2][{RuCl((S)-xylbinap)}2(?Cl)3]
Phlorizin dihydrate
6-Chloro-2-methyl-3-nitropyridine
Magnesium bromide
n-Dodecyl-beta-D-maltoside
1-Heptene
4-(DiMethylaMino)-1-(triphenylMethyl)pyridiniuM Chloride
6,7-DibroMobenzo(1,4)dioxan
Thionyl chloride, 99.7%
Oxtriphylline, USP
1-Chlorononane
2-(1-Naphthylmethyl)-2-imidazoline hydrochloride
4-(diphenylphosphino)-Benzaldehyde
4-Bromobenzothiazole
kanamycin sulfate mixture of components A
STRONTIUM STANNATE
FMoc-L-4-Fluorophe
Cyclohexane, HPLC Grade, 99.9+%
Poly(styrene sulfonic acid), M.W. 75,000, 30% w/v aq. soln.
Lead(II) chloride, ultra dry, 99.999% (Metals basis)
D-α-Cyclohexylglycine
2-Benzylphenyl isocyanate
N-ISOPROPYL-1,3-PROPANEDIAMINE
SaMariuM trifluoride
1-BroMo-2-(Methylsulfonyl)benzene
Acetic acid glacial
5-Amino-2-bromobenzoic acid
3-Phenyl, N1-BOC protected
z-asp(otbu)-osu
Dichlorotitanium Diisopropoxide
4-(2-METHYLPHENYL)-3-THIOSEMICARBAZIDE
3,4-DIMETHYLBENZAMIDE
Thioanisole
YttriuM;
SodiuM hypophosphite Monohydrate
4-Chloro-2-nitrophenol
4-(N-IsobutyrylsulfaMoyl)phenylboronic acid
DL-Tartaric acid, 99.5%
3-ChlorophenylMagnesiuM broMide, 0.5M solution in THF, AcroSeal
1,4-Diacetylbenzene
3,5-DiMethylheptane
N,N-dimethyldodecylamine-N-oxide
AZD-9291
6,7-Dihydro-5H-pyrrolo[3,4-b]pyridine
Triphenylethanediol
RHENIUM PENTACARBONYL BROMIDE
(11bS, 11′bS)-4,4′-(Oxydi-2,1-phenylene)bis-dinaphtho[2,1-d: 1′, 2′-f][1,3,2]dioxaphosphepin
3-Chloro-5-cyano-2-fluorophenylboronic acid, pinacol ester
1-[2-(4-CHLOROPHENOXY)-5-FLUOROPHENYL]-N-METHYLMETHYLAMINE
Hexadecyltrimethylammonium Chloride
Fumonisin B1 ,95%
Brilliant cresyl Blue-BB, certified, pure
2-Acetamido-2-deoxy-3-O-(β-D-galactopyranosyl)-D-galactopyranose ,95%
ALUMINUM N-BUTOXIDE
Mycophenolate mofetil
1-IODOOCTADECANE
2,5-DIMETHYLTHIOPHENOL
3-CyclohexylaMinopropanesulfonic acid
1,3-Bis(2-isocyanato-2-propyl)benzene
4-Butoxy-2,3,5,6-tetrafluorophenylboronic acid
alpha-BroMo-p-xylene, 98%
1-Benzyl-4-(4,4,5,5-tetraMethyl-1,3,2-dioxaborolan-2-yl)-1H-pyrazole, 97%
2-(2-Iodophenyl)-4,4,5,5-tetraMethyl-1,3,2-dioxaborolane
2-Iodobenzenesulfonyl Chloride
o-Nitrocinnamic acid
(S)-N-BOC-2-Bromophenylalanine
1-(3-NITRO-PHENYL)-ETHYLAMINE
palladium(ii) oxide hydrate
BrDU ELISA Kit
ARRb1 ELISA Kit
LPS/LOS ELISA Kit
Human Cyr61 (Cysteine Rich Protein, Angiogenic Inducer 61) ELISA Kit
Chicken Heat Shock Protein 60,HSP-60 ELISA Kit
Rat ICTP (Cross-linked C-Telopeptide of Type I Collagen) ELISA Kit
ET ELISA Kit
Human PRF1/PFP ELISA Kit
Human RSN ELISA Kit
MTF ELISA Kit
Yeast Mold Broth
Gause’s Synthetic Broth Medium,Modified
Ipa
MagnesiuM chroMate hydrate, 99.8% (Metals basis)
5-AMino-4,6-dichloropyriMidine, 98+%
2-Methyl-2-propyl-1,3-propanediol
2-(1-Naphthylmethyl)-2-imidazoline hydrochloride
Mercury chloride
5-Amino-1,2,4-thiadiazole
7-Chlorotryptophan
BISMUTH HEXAFLUOROPENTANEDIONATE
2,2'-THENIL
Ethyl 3-broMo-4-cyanobenzoate
N-Benzyloxycarbonyl-L-serine benzyl ester, 99%
Tri-n-butylethylphosphoniuM ethyl sulfate
2-Allyloxytetrahydropyran
Manganes Solution
4-Chloro-6-(pyrrolidin-1-yl)pyriMidine
(4-Fluorophenylethynyl)triMethylsilane
Tris(3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctyl)tin hydride
(S)-(-)-4-BroMo-alpha-phenylethylaMine
Bis(2-methoxyethyl) ether, extra pure, 99%
2,2'-(1,2-Ethenediyldi-4,1-phenylene)bisbenzoxazole, 97%
4-Methyl-1-naphthoic Acid
4-Methylbenzyl Phenyl Ketone