SW1990细胞系—人癌细胞系国外引进 货号:GD-C16718941
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结:1)培养基中血清的比例多少合适?血清是细胞培养上一个极为重要的营养来源,所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%,不要低于5%或高过20%,因为含量过低会导致细胞营养不足,过高则会破坏渗透压平衡。一般建议血清含量为10%,可以适量加减;2)何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上的更换时间,按时更换培养基即可。还可以参照指示剂的颜色变化进行更换;3)购买的复苏细胞系—为何会有脱落?因为运输的过程中不可避免的会有震荡,收到后可以离心收集;4)购买的细胞冷冻管经解冻后,为何会发生细胞数目太少之情形?研究人员在冷冻细胞之培养时出现细胞数目太少,大都是因为离心过程操作上的失误,造成细胞的物理性损伤,以及细胞流失。建议细胞解冻后不要立刻离心,应待细胞生长隔夜后再更换培养基即可;5)购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,常见原因可归纳为:培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后,加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久;6)收到的冷冻管瓶身破裂,瓶盖有裂纹,或瓶盖脱落之原因?冷冻管瓶盖裂纹,或瓶身破裂,可能是因为操作者夹取冷冻管时用力不当,造成冷冻管裂损,建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱,乃因热胀冷缩之物理现象,冷冻管有可能因此而造成细胞污染,故冷冻管于放入和取出液氮桶时,均应立刻将冷冻管再一次扭紧。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3-(2,3-DiMethylphenylcarbaMoyl)benzeneboronic acid, 96%
SodiuM phosphate dibasic dodecahydrate
2,4-Pentanedione
PRASEODYMIUM(III) CHLORIDE HYDRATE, 99.90%
3'-BroMoacetophenone
CALCIUM LACTOBIONATE
2-Methoxy-5-Methylbenzoic acid
Ethyl 4,6-dichloronicotinate ,97%
4-{[(4-Methoxybenzyl)carbamoyl]amino}benzeneboronic acid, pinacol ester 98%
1,2,6,7-Tetrahydro-8H-indeno[5,4-b]furan-8-one
Hydroquinidine Hydrochloride
4-METHOXY-3-NITROBENZALDEHYDE
TRANS-2-(4-FLUOROPHENYL)VINYLBORONIC ACID
2-Chlorobenzoyl chloride
Tributyl(vinyl)tin
DL-BroMosuccinic acid
5-(Diethylphosphono)-5-Methyl-1-pyrroline N-Oxide
4,5-DibroMo-1-Methyl-1H-iMidazole
trans,trans-2,4-Decadienal, 95%, tech.
PheniraMine Maleate, BP
Methyl 2-AMinoisonicotinate
m-hydroxybenzenesulphonic acid
3-Chloro-2-Nitrotoluene
1-Dehydrotestosterone
Aminoacetonitrile bisulfate
2'-HYDROXY-1'-ACETONAPHTHONE
DEVARDA'S ALLOY
2-Chlorobenzyl Alcohol
PotassiuM acetate, 99%
8-Hydroxyjulolidine, 97%
2,4,5,6-TetraaMinopyriMidine sulfate salt
Methyl heneicosanoate
ASTRAZON ORANGE R
RhodaMine B
4-FLUORO-2-(TRIFLUOROMETHYL)CINNAMIC ACID
3-(4-Chlorophenylthio)butyric acid, 95%
1-Adamantanecarboxamide, 98%
1-(2-Hydroxyethyl)-4-methyl, 98%
4-Amino-1H-imidazole-5-carbonitrile
Amyl Benzoate
4-PHENOXYBENZONITRILE
P-XYLYLENEDIPHOSPHONIC ACID TETRAETHYL ESTER
4-(TRIFLUOROMETHOXY)BENZOIC ACID HYDRAZIDE
(R)-N-Boc--2-carboxylic acid methyl ester
1,1,2,2-Tetrachloroethane-d2
4-AMino-3-chlorobenzonitrile
4'-Ethylpropiophenone
2,3,5-Trichloroisonicotinaldehyde
2-BroMopyridine-3-carboxylic acid, 97%
CHLORODIBROMOACETIC ACID
2-PhenylcarbaMoyl-1,4-naphthoquinone-4-(4-diethylaMino-2-Methylphenyl)iMine
Tylosin, [R-(R*,R*)]-2,3-dihydroxybutanedioate (salt)
Zirconium fluoride;Zirconium tetrafluoride
4-Nitrothiophenol;4-Nitrophenyl mercaptan
2-Chloro-4-nitrophenol
2,3-Difluoroanisole
Indazole-4-boronic acid
3-HydroxyMandelic Acid
5-Methyl-3-phenylisoxazole-4-carbonyl chloride
2-Aminoethyl 2-chloro-6-fluorobenzyl sulphide 97%
(R,R)-2,6-Bis(4-phenyl-2-oxazolin-2-yl)pyridine
Fmoc-N-Me-lle-OH
2-Pyrrolidone-5-carboxylic acid, sodium salt, 50 wt% solution in water
1-(2,4,6-TRICHLOROPHENYL)-2-THIOUREA
3,5-Lutidine
CHOLESTERYL HEPTANOATE
1,4-DibroMobenzene;p-Benzene dibroMide
1-BroMononane
1,3-Dichloro-5-fluorobenzene
4-Nitro-o-cresol
Z-N-Me-ile-oh
MethyltriphenylphosphoniuM iodide, 97%
FIR NEEDLE OIL, CANADIAN
Monoolein
Diisopinocampheylborane
3-Iodobenzyl bromide
Solvent Red 207
2-Chloroadamantane
CT1 ELISA Kit
β-EP ELISA Kit
Human regulated on activation in normal T-cell expressed and secreted,RANTES ELISA Kit
Human CLDN5 ELISA Kit
IGY ELISA Kit
NDRG2 ELISA Kit
NTX ELISA Kit
Human ER ELISA Kit
ANG1-7 ELISA Kit
CYTH1 ELISA Kit
Trichophyton Agar #3
Oxacillin Resistance Screening Agar Base
Ethyl Mesitylacetate, 97%
BariuM zirconiuM oxide, 99% (Metals basis)
5-AMino-2-chlorobenzoic acid, 98+%
2-Cyclopentylethanol
m-hydroxybenzenesulphonic acid
1-Propanesulfonyl chloride
Ethyl (phenylthio)acetate
2,4-Bis(trifluoromethyl)chlorobenzene
BIS(N-PROPYLTETRAMETHYLCYCLOPENTADIENYL)BARIUM
1,1'-DIOCTADECYL-3,3,3',3'-TETRAMETHYLINDOCARBOCYANINE PERCHLORATE
(2-BroMobenzyl)MethylaMine
Tetra-n-butylaMMoniuM tetraphenylborate, 99+%
2-n-Butyl-5-ethylthiophene, 97%
3,4-Difluorophenol
4-Ethoxy-2,3-difluorophenol,JRD
7-Fluoro-3-(hydroxyiMino)indolin-2-one
N-ETHYL-P-TOLUIDINE
2-IODONONAFLUOROBUTANE
1-Hydroxybenzotriazole Hydrate
(4-Oxo-4 H -quinazolin-3-yl)-acetic acid
Perfluoroprop-1-ene
Yttrium(III) acetylacetonate hydrate (99.9%-Y) (REO)
1,4-Hexadiene (cis- and trans- mixture)