U27KM细胞系*小鼠子宫颈癌细胞系培养ZX 货号:GD-C15920576
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系*全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞系*将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
4-n-Decylbiphenyl, 98%
3-Ethyltoluene
2-N-PROPYLPROPANE-1,3-DIOL
(S,S)-2,2′-Bipyrrolidine D-tartrate trihydrate
1,5-HEXADIYNE
RHODIUM (III) BROMIDE
3-OCTANOL
4-(Fur-2-yl)benzoic acid
Boc-3-Methoxy-D-phenylalanine
(6-Chloro-2-phenyl-4-pyrimidinyl)methyl methyl ether
4,4'-Hexamethylenebis(1,1-dimethylsemicarbazide)
3,5-DINITROBENZYL ALCOHOL
trans-4-Methycyclohexyl isocyanate
2-Chloro-3-fluoro-5-methylpyridine
2-FLUORO-5-METHYLPHENOL
[5-Methyl-2-(trifluoroMethyl)-3-furyl]MethylaMine
4-DiMethylaMino-4'-nitrostilbene
5-BroMopyridine-3-sulfonaMide
Styrene, 99%, extra pure, stabilized
Mercaptopurine, Monohydrate, USP
N-Carbobenzoxy-D-leucine DicyclohexylaMMoniuM Salt
Oxamide
6-chloropyrimidine-4,5-diamine
1,5-Dichloropentane
Acetonesemicarbazone
1-IODODECANE
Phenylfluorone
RutheniuM(III) Chloride Hydrate
Apatite, naturally occurring Mineral, grains, approxiMately 0.06-0.19in
(R)-(-)-1,2-Propanediol, 98%
PotassiuM phosphate dibasic
2-AMino-5-broMo-4-thiazolecarboxylic acid Methyl ester
Chloroacetonitrile
tert-Butyl 3-benzyloxy-4-broMobenzoate
HexylMagnesiuM chloride solution
TRIS[N,N-BIS(TRIMETHYLSILYL)AMIDE]NEODYMIUM (III)
3-Methyl-indan-1-one
2,4-Dinitro-5-fluoroaniline 97%
(1S)-(-)-1-[3,5-Bis(trifluoromethyl)phenyl]ethan-1-ol
alpha,alpha,alpha'-Tris(4-hydroxyphenyl)-1-ethyl-4-isopropylbenzene
2,3,4-TRIFLUOROPHENOL
4-(1,1,2,2-TETRAFLUOROETHOXY)ANILINE
Lead naphthenate
2-Nitroso-1-naphthol;1,2-Naphthoquinone-2-oxiMe;1,2-NaphthoquinoneiMine-2-oxiMe;C.I. 10010
Fast Blue BB
2-AMino-4-Methylbenzonitrile
Azoic Diazo CoMponent 48
3-AMino-5-broMopyridine-2-carboxylic acid
Benzyl phenyl sulfone, 98%
P-TERPHENYL-D14
2,4,6,8-TetraMethylcyclotetrasiloxane
2-cyano-N-(3-fluorophenyl)-Acetamide
7-ketolithocholic Methyl ester
2-tert-Butyl-4-methylphenol
6-(dimethylamino)fulvene
LEAD(II) SELENATE
N-HydroxysucciniMide
2-(AcetylaMino)-3-phenyl-2-propenoic acid
2-Nitro-4-(trifluoroMethyl)phenyl isocyanate
2-Methylamino-1-morpholin-4-yl-ethanonehydrochloride
DL-Isoserine
2,2'-Dihydroxydiphenyl Ether
5,6-Dimethoxybenzimidazole
3,5-Dichlorobenzaldehyde
4-TRIMETHYLSILYL-3-BUTYN-2-OL
PHYSOSTIGMINE
2-Chloro-4,6-dimethoxypyrimidine
2,5-Dichlorothiophene-3-carbonyl chloride
1-(3-BroMopropyl)pyrrole
(3,5-DiMethyl-1-phenyl-1h-pyrazol-4-yl)acetic acid
AMMoniuM sulfate, 99.5%, for analysis
4'-n-Butylacetophenone, 95%
6-(DibutylaMino)-1,3,5-triazine-2,4-dithiol
Triphenylsilane
3-Chloropropionyl chloride
N6-benzoyl-2'-deoxyadenosine hydrate
CIPROFLOXACIN LACTATE
Benzene, 1,2-bis(bromomethyl)-3-nitro-
NO ELISA Kit
IL21 ELISA Kit
TNFβ ELISA Kit
Human GAPDH/G3PDH ELISA Kit
IL8 ELISA Kit
Rat Cluster of differentiation 3,CD3 ELISA Kit
Monkey IL-16 (Interleukin 16) ELISA Kit
Human OPAs ELISA Kit
Human ANF ELISA Kit
NAIP ELISA Kit
Gram Negative Enrichment Broth
Mn2+ Nutrient Agar
TriMethyl orthobenzoate, 98%
PotassiuM Persulfate
5-Hexyn-3-ol, 98+%
(+/-)-2-Methyl
Oxamide
2-Propenoic acid, 3-phenyl-, 1-methylethyl ester
3-amino-N-ethylpiperidine
5-Iodosalicylic acid
Cesium carbonate
Triethyl orthoacetate
3,4-Dichlorobenzoyl chloride, 97%
5-BroMo-7-nitroindoline, 98%
Tetrahydro-2H-pyran-4-ol
8-ACETOXYQUINOLINE
(1S)-3,3-Di(phenanthren-9-yl)-1,1-binaphthyl-2,2-diol
Dihexyl sulfosuccinate sodiuM salt solution
ETHYL 2-BENZYLACETOACETATE
4-(Morpholinomethyl)benzaldehyde, 97%
1-(tert-Butyl)-3,5-dimethyl-1H-pyrazole-4-carbaldehyde, 97%
1-(4-Trifluoromethylphenyl)
5-Nitropyridine-3,4-diamine