NCI-H441[H441]细胞系*人肺腺癌细胞系培养ZX

NCI-H441[H441]细胞系*人肺腺癌细胞系培养ZX 货号：GD-C15920415
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系*全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息，我们拥有丰富的细胞系培养经验，能提供细胞培养全方位的技术支持，让您实验再无烦扰！公司由在国外工作和学习多年的留学人员创办，由国内ZG科学院上海生物化学与细胞生物学研究所，复旦大学，同济大学等多名工作在线的细胞培养专家，提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念，以世界的技术支撑阵容，提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美，提供了重要的基石；公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品，立足国内，面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位，为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内，在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室，以便更好的服务国内广大医院、大学、制药企业等，你们的每一份关心和支持，都是我们前进的动力！
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞系*将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Chlorotriethylsilane, 98+%
(S)-(?)-2,2′,6,6′-TetraMethoxy-4,4′-bis[di(3,5-xylyl)phosphino]-3,3′-bipyridine
4-Hydroxyisophthalic acid
1-Benzyl-4-cyano-4-phenylpiperidine hydrochloride
N-Isopropyl 3-aMinobenzenesulfonaMide
2-BroMo-3-pyridyl trifluoroMethanesulfonate
4,6-DiMethyl-2-MethylMercapyriMidine
4-[(6-Methylpyrazin-2-yl)oxy]benzyl alcohol 97%
3-Acetylindazole
Sodium Propionate
Praseodymium(III) chloride heptahydrate (99.9%-Pr) (REO)
4-ACETOXYCINNAMIC ACID
Glycyl-L-glutamine monohydrate
(+)-CIS-2-BENZAMIDOCYCLOHEXANECARBOXYLIC ACID
HoMo
2-(AMinoMethyl)piperidine
2,5-Dihydroxyterephthalic Acid
Methyl 5-broMo-2-hydroxynicotinate
Zinc trifluoroMethanesulfonate, 98%
Sucralfate, Fine Powder, USP
CooMassie Brilliant Blue R-250 [for Electrophoresis]
3-Methylbenzophenone
5-(hydroxymethyl)furan-2-carboxylic acid
4-Anilino-1-benzylpiperidine
4-fluorobenzyl bromide
3,5-Bis(trifluoromethyl)benzaldehyde
Methyl 4-formylbenzoate
Bis(4-nitrophenyl) phosphate
Ethyl (S)-1-Boc-5-oxopyrrolidine-2-carboxylate, 98%
4'-Fluoro-2'-Methoxyacetophenone, 97%
Propionic acid
(R)-1-Benzyl-3-(Boc-aMino)pyrrolidine
6-AMino-3-chloropicoline
OCTAMETHYLCYCLOTETRASILAZANE
Dodecanenitrile
1,3-BIS(ISOCYANATOMETHYL)CYCLOHEXANE
Trimethylsulfoxonium iodide, 98%
Fmoc-L-4-pyridylalanine
Chloromethylphenylsilane, 93%
3-(Pyrrolidin-1-yl)benzylamine 90%
2,5-DIFLUOROPHENYLACETONITRILE
Tetraethylammonium iodide
4-Phenoxybutyl bromide
Polysucrose
(5-BroMo-1-benzofuran-2-yl)Methanol
5-(Benzylthio)-1H-tetrazole
Ethyl 4-[(4-Methoxybenzylidene)aMino]cinnaMate
3-Chloro-2-Methyl-4-thiocyanatoaniline
4-(BroMoMethyl)-3-Methyl-5-phenylisoxazole, 95%
N-METHOXYSUCCINYL-ALA-ALA-PRO-VAL P-NITROANILIDE
(2S,3S)-1,2-Epoxy-3-(tert-butoxycarbonylaMino)-4-phenylbutane
Invertose
allura red ac
4,4'-sulphonylbis[2-aminophenol]
Cesium formate
3-CHLORO-2-FLUORO-6-(TRIFLUOROMETHYL)BENZALDEHYDE
FEMA 2296
1,5,6,7-TETRAHYDRO-2-METHYL-4H-INDOL-4-ONE
(1R,2R)-[N,N′-Bis(2′,3′-dihydroxybenzylidene)]-1,2-diaMinocyclohexane
4,4'-Dichlorodiphenyl sulfone, 97%
(3-Glycidoxypropyl)methyldimethoxysilane
2-Methoxyphenylacetonitrile
4-(Dimethylamino)phenylacetic acid ,97%
SILVER PERCHLORATE
R-(-)-Fluoxetine hydrochloride
2,4,6-TRINITROANILINE
2-Formylthiophene-3-boronic acid
2-(4-Thiazolyl)benziMidazole
1-BroMo-4-heptylbenzene (stabilized with Copper chip)
DiMethyl 2-(3-nitropyridin-2-yl)Malonate
AMMoniuM iron(III) sulfate dodecahydrate, 99+%, for analysis
Acetyl-beta-Methylcholine chloride, 98%
Cinchonine Hydrochloride Hydrate
5-Chloro-o-cresol
3-amino-3-methyl-butanoic acid
2-(trifluoromethyl)pyrimidine-5-carbaldehyde
Hydroxypropyl-beta-cyclodextrin
Potassium naphthenate
MAPK ELISA Kit
S-100 ELISA Kit
Human PCDHβ12 ELISA Kit
Human FXR ELISA Kit
S100A6 ELISA Kit
Rat β2-microglobulin,BMG/β2-MG ELISA Kit
Chicken Anti-Thrombin Ⅲ,AT-Ⅲ ELISA Kit
Human Cholic acid,CA ELISA Kit
Human GSK2 ELISA Kit
ACC ELISA Kit
Cepulodin Irgasan Novobiocin Agar Base
Alkaline Bile Salt Agar
Tri-O-acetyl-D-glucal
Lead(II) Fluoride, Puratronic (Metals Basis)
5,5'-Dithiobis(2-nitrobenzoic acid), 99%
2-Fluoro-4-(1-piperidinylcarbonyl)benzeneboronic acid, 97%
3-Methylbenzophenone
dibismuth trimolybdenum dodecaoxide
Enocyanin
Methionic acid

2,4-DIAMINO-6-MERCAPTOPYRIMIDINE
BIS(DICYCLOHEXYLPHOSPHINO)METHANE
4-AMinobenzoic Acid
Mercury(II) sulfide, black
2-Fluoropyridine-3-boronic acid pinacol ester, 95%
5-Chloro-1H-pyrrolo[2,3-b]pyridine
PCB No 15
4-(DiMethylaMino)phenylboronic acid, HCl
PHENACYL 4-BROMOCROTONATE
2-BroMophenol
Tetrachlorosilane
4-(1,3-Thiazol-2-yl)benzaldehyde, 95%
3-Chlorothiophene-2-carboxylic acid
Hyaluronic Acid from Cockscomb
5-Bromo-2-hydroxypyrimidine