KG-1a细胞,人急性白血病细胞系用哪个培养基 货号:GD-C14418294
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞冻存的步骤:1)选择处于对数生长期的细胞,在冻存前一天Z好换液。将多个培养瓶中的细胞培养液去掉,用0.25% 胰蛋白酶消化。适时去掉胰蛋白酶,加入少量新培养液。用吸管吸取培养液反复吹打瓶壁上的细胞,使其成为均匀分散的细胞悬液。悬浮生产细胞则不要消化处理。然后将细胞收集于离心管中离心(1000r/min,10分钟);2)去上清液,加入含20%小牛血清的完全培养基,于4℃预冷15分钟后,逐滴加入已无菌的DMSO或甘油,用吸管轻轻吹打使细胞均匀,细胞浓度为5×106~1×107/mL之间。(冻存培养液的配置是不是一定要用小牛血清呢?答案是不一定的,具体要看培养的细胞类型来选择;3)将分装上述细胞悬液于2ml冻存管中,每管0.25ml。冻存管要将盖子盖紧,并标记好细胞名称和冻存日期,同时作好登记(日期、细胞种类及代次、冻存支数);4)将装好细胞的冻存管放到冻存盒中,-80℃冰箱过夜。;如果有程序降温器(放在-80℃冰箱过夜,放入液氮罐)Z好;或者可以在4℃,2h;然后转到-20℃,2h;-80℃,2h;放入液氮罐;5)细胞冻存在液氮中可以长期保存,但为妥善起见,冻存半年后,Z好取出一只冻存管细胞复苏培养,观察生长情况,然后再继续冻存。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3-(trans-3-Ethoxy-3-oxo-1-propen-1-yl)benzeneboronic acid, 98%
Furfuryl acetate
(S)-(6,6′-DiMethoxybiphenyl-2,2′-diyl)bis(diisopropylphosphine)
1-Chloro-2-butene
DANSYL HYDRAZINE
2-BROMO-6-METHYL-4-NITROANILINE
4-Methyl-1-phenyl-2-pentanone
Dipropylamine
2,6-Dimethyl-1-heptene
methyl 2-(3-formyl-2,5-dimethyl-1H-pyrrol-1-yl)benzoate(SALTDATA: FREE)
1-Cyclopentenylboronic acid pinacol ester
N-(4-BROMOPHENYL)-2-CHLOROACETAMIDE
3-(1,1,2,2-TETRAFLUOROETHOXY)ANILINE
Potassium oxonate
1,4-Butane sultone
Methyl 3-aMinobenzoate
Disperse Diazo Black 3BF
5-BroMo-2-Methoxy-N-MethylbenzenesulfonaMide
Poly(vinyl acetate), sec. stand., typical M.W.184100, typical M.N.61600
Fluconazole, USP
TetrapropylaMMoniuM Iodide
6-hydroxy-2-naphthalenesulfonic acid sodium salt
methyl 4-ethynylbenzoate
quinolinium hydrogen sulphate
2,2'-Azobis(2-methylpropionitrile)
2'-BROMO-4'-METHYLACETANILIDE
3-Methoxythiophene
6-Heptenoic acid, 98%
N-n-Butyl-N-MethylethanolaMine, 96%
StrontiuM chroMate
2-AMino-6-fluorobenzonitrile, 99%
YTTRIUM(III) TRIFLUOROMETHANESULFONATE
Zinc acrylate
P-TOLUENESULFONIC ACID (S)-2-METHYLBUTYL ESTER
Methyl cyanoforMate
5-Fluoro-2-oxindole 98%
N-(2-Cyanoethyl)-N-ethylaniline
Hellebrin, Helleborus sp.
(R)-(-)-1-[(S)-2-(Di-1-naphthylphosphino) ferrocenyl]ethyldi-3,5-xylylphosphine, min. 97%
3-Tolylboronic acid
TETRAMETHYLENEBIS(TRIPHENYLPHOSPHONIUM BROMIDE)
4-Bromo-3-(trifluoromethyl)aniline
4-Nitroanisole
6-BroMoisoquinoline
Methyl 3-Cyanopropionate
4-Methoxy-2-nitrophenylboronic acid, pinacol ester
Ethyl ethynyl ether, 40 wt.% solution in hexanes, AcroSeal
Tetrahydropyran-4-ylacetaldehyde, 95%
N-tert-ButylMethacrylaMide (stabilized with MEHQ)
Tiglic Anhydride
4-n-Heptylphenol
5,5'-dihydroxy-5,5'-bipyrimidinehexaone
5-Ethoxycarbonyl-4-(trifluoromethyl)pyrimidin-2(1H)-one
9-(2'-ACETOXYETHOXYMETHYL)-GUANINE
2-Methylpyrazine
1-ethyl-3-MethyliMidazoliuM dicyanaMide
5,5-Diphenylhydantoin
DiethylenetriaMinepentaacetic acid
1H,1H,2H,2H-Perfluorooctyltriethoxysilane
(Methylsulphonyl)methanesulphonyl chloride
Hexadecyltrimethylammonium bromide, 99+%
Zinc phosphate hydrate
(S)-(+)-KETOPINIC ACID
3-CHLORO-2-FLUOROBENZOYL CHLORIDE
5-Bromothiazole
1,1,2,2-TetrabroMoethaneBr2HCCHBr2;Acetylene tetrabroMide;MuthMann's liquid;TBE
1-BroMo-4-phenylbutane
(+)-Dibenzoyl-D-tartaric Acid Monohydrate
Nicardipine Hydrochloride
2-(2-Morpholinoethoxy)-1,3-dichlorobenzene
Bis(pentafluorophenyl) carbonate, 98%
BZ-TYR-PNA
9-Phenylxanthen-9-ol
6-Amino-5-nitro-2-picoline
Glycyl-L-Histidyl-L-Lysine
(S)-Ethyl 1,2,3,4-tetrahydroisoquinoline-3-carboxylate hydrochloride
phenol-2,4,6-D3
TBA ELISA Kit
CFH ELISA Kit
Human Cathepsin K,cath-K ELISA Kit
Human OPG ELISA Kit
4-NT ELISA Kit
HDL ELISA Kit
Vwf ELISA Kit
Human GAL2 ELISA Kit
Human Cytochrome P450c21/21-hydroxylase,CYP21B ELISA Kit
SICAM1 ELISA Kit
Letheen Broth Base ,Modified
Kassanis Medium
ALPHAZURINE A
Guaiacwood oil
5-BroMo-2,4-dichloropyriMidine
2-Hydroxypyridine, 98+%
6-hydroxy-2-naphthalenesulfonic acid sodium salt
Brilliant Blue R
L-Ornithine Alpha-Ketoglutarate (1:1) Dihydrate
Methacrylic acid
2-(4,4,5,5-TETRAMETHYL-1,3,2-DIOXABOROLAN-2-YL)PHENOL
BIS(DIMETHYLAMINO)PHOSPHORYL CHLORIDE
Trans-4-Propylcyclohexanecarboxylic Acid
RheniuM(VII) oxide, 99.995% (Metals basis)
Octafluorotoluene, 98+%
2-MethylcyclohexylaMine(cis + trans)
AcetaMide
3,4-Dichloronitrobenzene
N,N-DIETHYLALLYLAMINE
1-BroMo-4-cyclohexylbenzene
Dichlorobis(2-(diisopropylphosphino)-ethylaMine)rutheniuM(II)
3,4-Dihydro-3-oxo-2H-1,4-benzothiazine-6-carboxylic acid
Ethyl 2-chloroisonicotinate
3-(1-methyl-1H-pyrrol-2-yl)-1H-pyrazole-5-carboxylic acid(SALTDATA: FREE)
5-Bromo-4-chloro-2-hydroxybenzoic acid 96%