HDMEC细胞株|人微血管内皮细胞系 货号:GD-C9318887
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结:1)培养基中血清的比例多少合适?血清是细胞培养上一个极为重要的营养来源,所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%,不要低于5%或高过20%,因为含量过低会导致细胞营养不足,过高则会破坏渗透压平衡。一般建议血清含量为10%,可以适量加减;2)何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上的更换时间,按时更换培养基即可。还可以参照指示剂的颜色变化进行更换;3)购买的复苏细胞,为何会有脱落?因为运输的过程中不可避免的会有震荡,收到后可以离心收集;4)购买的细胞冷冻管经解冻后,为何会发生细胞数目太少之情形?研究人员在冷冻细胞之培养时出现细胞数目太少,大都是因为离心过程操作上的失误,造成细胞的物理性损伤,以及细胞流失。建议细胞解冻后不要立刻离心,应待细胞生长隔夜后再更换培养基即可;5)购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,常见原因可归纳为:培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后,加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久;6)收到的冷冻管瓶身破裂,瓶盖有裂纹,或瓶盖脱落之原因?冷冻管瓶盖裂纹,或瓶身破裂,可能是因为操作者夹取冷冻管时用力不当,造成冷冻管裂损,建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱,乃因热胀冷缩之物理现象,冷冻管有可能因此而造成细胞污染,故冷冻管于放入和取出液氮桶时,均应立刻将冷冻管再一次扭紧。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Diethyl 5-hydantoylphosphonate, 96%
1,1'-ThiocarbonyldiiMidazole
9-ACETYLANTHRACENE
2-Carboxypyridine-4-boronic acid
TIN(IV) TERT-BUTOXIDE
5-Isocyanatoindane
CALMAGITE
Cyclobutanecarboxylic acid ,98%
Methyl 1-methyl-1H-indazole-6-carboxylate
Formaldehyde, reagent ACS, 37 wt% solution, stabilized
Yttrium(III) acetate hydrate (99.9%-Y) (REO)
5-CHLORO-1,10-PHENANTHROLINE
(1S,2R)-1-PHENYL-2-(1-PYRROLIDINYL)PROPAN-1-OL
1,2-EPOXY-9-DECENE
3-Azetidinecarboxylic acid
5-AMino-3-Methylisoxazole
2,4'-DiMethoxybenzophenone
N-Cyclopentyl 3-broMo-5-nitrobenzaMide
ThiaMine hydrochloride, 98.5-101.5%
Pepsin, 1:3,000, Powder
cis-Decahydro-1H,6H-3a,5a,8a,10a-tetraazapyrene
4,4',4''-methylidynetrianiline
8-benzyl-1-oxa-3,8-diazaspiro[4.5]decan-2-one
3-Chlorophenylacetylene
Phenyl 4-aminosalicylate
1-ETHYL-4-FLUOROBENZENE
FMoc-L-Phenylglycine
p-Tolylacetonitrile, 98+%
(S)-(-)-5-HydroxyMethyl-2(5H)-furanone, 98%
6-MethyliMidazo[1,2-a]pyridine, HCl
N,N′-DiMethyl-1,8-octanediaMine
ScandiuM(III) tris(2,2,6,6-tetraMethyl-3,5-heptanedionate) hydrate
ETHYL DIBROMOACETATE
1-(2-CHLOROPHENYL)BIGUANIDE HYDROCHLORIDE
2'-Methylbiphenyl-4-carboxylic acid
2-(4-Bromo-phenyl)quinoline-4-carboxylic acid
Fmoc-3,4-dehydro-L-proline
O-Acetyl-N-carbobenzoxyhydroxylamine
Armillarisin A
16-Dehydropregnenolone acetate
2-AMINO-1-NAPHTHOL HYDROCHLORIDE
2-(Trifluoromethyl)benzyl alcohol
1-BroMo-2,4,5-trifluorobenzene
4-CarboxyMethyl-6,7-MethylenedioxycouMarin
5-Methylpyridine-3-boronic acid pinacol ester
FuMaryl chloride, 95%
3-HydroxyadaMantane-1-carboxylic acid, 97%
alpha-BroMo-4-chlorophenylacetic Acid
AllylchlorodiMethylsilane [AllyldiMethylsilylating Reagent for GC/MS]
ethyl 6-bromo-2-oxo-2H-chromene-3-carboxylate
choline bitartrate
Celecoxib
5,7-difluoro-3,4-dihydronaphthalen-1(2h)-one
2-Chlorocyclopentanone
4-Chlorobenzhydrylchloride
4,4'-(1-Phenylethylidene) biphenol
2,2,5-TriMethyl-1,3-dioxane-4,6-dione
2-Deoxy-L-ribose, 99%
(4-chlorophenyl)methanesulfonyl chloride(SALTDATA: FREE)
3-Bromo-5-methoxyaniline 98%
3,5-Dipropyl-1,2,4-triazol-4-ylamine
Phenethyl isocyanate
6-Bromoindazole
Fmoc-O-tert-butyl-D-serine
Carbon disulfide;Dithiocarbonic anhydride
2-BroMo-3,3,3-trifluoro-1-propene (stabilized with copper chip)
1,3-Benzodioxole
Isorhapontigenin
4-BroMo-2-nitro-5-(trifluoroMethyl)aniline
beta-NicotinaMide adenine dinucleotide hydrate, 98+%
H-DL-TIC-OH
9-BroMotriptycene
ethyl 2-cyanopentanoate
dimethyl isopropylidenemalonate
N-E-Z-L-lysine benzyl ester hydrochloride
4-(Diethylamino)butan-1-ol
ACE I ELISA Kit
IFNγ ELISA Kit
Human LIPI ELISA Kit
Human CAMK2α (Calcium/Calmodulin-Dependent Protein Kinase II Alpha) ELISA Kit
GFAP ELISA Kit
Col I ELISA Kit
sIgA ELISA Kit
Human VLA (Very Late Appearing Antigen) ELISA Kit
Human Androgen Binding Protein,ABP ELISA Kit
SA ELISA Kit
MEA Medium
ISP Medium 2(Yeast Extract Malt Extract Agar)
3-IndoleforMic Acid
Phlorizin
Ethyl 5-AMino-1H-Pyrazole-3-Carboxylate
2-Hydroxyisobutyric acid
4,4',4''-methylidynetrianiline
lithium metasilicate
5-Vinylpyrrolidone
betamethasone 21-phosphate sodium
(S)-(-)-N-ISOPROPYL-1-PHENYLETHYLAMINE HYDROCHLORIDE
2-AMino-5-Iodopyridine
DodecacarbonyltetrairidiuM, 98%
Ethyl thiophene-2-carboxylate, 98+%
DiethoxyMethylphenylsilane, 97+%
pH standard-Borax
ETHYL 3-BENZOYLACRYLATE
DIBENZO-15-CROWN-5
Sulfonazo III tetrasodiuM salt
4,4'-BIS(DIMETHYLAMINO)BENZIL
3,5-DibroMobenzyl broMide
5,7-Dimethylisatin, 95%
3-Aminobenzyl alcohol
2-Fluoro-4-iodobenzaldehyde
4-Isopropylpyrimidine-5-boronic acid 95%