NUTU-19细胞株:大鼠卵巢癌细胞系培养ZX 货号:GD-C8919709
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
养了几年细胞,说一点自己培养细胞方面的看法:1)培养细胞前,可以看看细胞特点说明书,包括细胞正常生长的状态图、传代、培养基的类型等;2)不同细胞生长周期不同,有的生长很快,比如说MDA-MB-231,传代的时候取离心悬液的十分之一就已经足够了,有的又是特别慢,等的人心焦,BT474就是,传代是一分二,复苏起始的时候两周多才能长满,所以就要在培养细胞的过程中多多摸索了;3)对于娇弱的细胞,就得细心呵护了,胰酶消化不能过久,吹得时候要轻柔,离心时候也要注意转速和时间,每天都要去看一下细胞,肉眼观察培养基状况、显微镜下观察细胞生长状况;4)冻存细胞复苏后第二天Z好更换一次培养基;5)培养箱隔一段时间彻底用酒精棉擦洗一次;6)养细胞的教训:不能偷懒!细胞虐我千百遍,我待细胞如初恋。1)不管买的细胞、惠赠的细胞,刚拿到手赶紧做两件事:检测是否有支原体污染; 迅速扩增冻存;2)发现有污染迅速处理掉,特别是当你养了很多种细胞时; 细菌污染,真菌污染很容易发现,支原体污染的话,细胞会长的慢,买个支原体检测的kit定期检测一下;3)传代细胞日常的值日清洁是必须的,此外还要定期熏蒸;4)细胞的传代一定要规律,保持相同的confluency和传代时间间隔,不要随心所欲或者拖延;5)注意个人卫生。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2,5-Difluorophenylhydrazine
Myricetin
4-(4-Methoxyphenyl)-2-butanone
4-(1H-Pyrazol-1-yl)phenylboronic acid
Hexanoic acid
Poly(vinyl chloride)
4-(Trifluoroacetyl)benzoic acid 97+%
2,4-Dichloropyrimidine-5-carboxylic acid
2-Chloro-6-fluorotoluene, 97%
Boc-3-amino-4-chlorobenzoic acid
4-Amidinophenylmethanesulfonyl fluoride hydrochloride
Isoxazole
Ethyl vinyl ketone
4-(4-ETHOXYPHENYLAZO)-M-PHENYLENEDIAMINE MONOHYDROCHLORIDE
Methanol-d4
4-AMino-3-hydroxybenzoic acid
N-[(9-Ethylcarbazol-3-yl)Methylene]-2-Methyl-1-indolinylaMine
4-Chloro-2-hydroxybenzonitrile
2,4-DibroMothiophene, 96%
DIETHYLAMINE
Bis(4-nitrophenyl) Disulfide
5-BROMOPYRIMIDIN-4-AMINE
carbonic anhydrase from bovine erythro-cytes
Toluene-d8
phenyl bromoacetate
Isopropylboronic acid
PSEUDOPELLETIERINE HYDROCHLORIDE
Benzyl (S)-(-)-Tetrahydro-5-Oxo-3-FuranylcarbaMate
6-Fluoro-1-indanone, 97%
2,3,4,5-Tetrafluorobenzyl broMide, 97%
PotassiuM hydroxide
Diethyl Chlorooxalacetate
5-BroMo-2-nitropyridine
N-(Thiazoline-2-yl) 4-boronobenzaMide
LithiuM triisobutylhydroborate
5-((BOC-AMino)Methyl)furan-2-boronic acid
1-(3-methyl-2-pyridinyl)ethanamine(SALTDATA: FREE)
Thiophene-3-boronic acid
alpha,alpha,alpha',alpha'-Tetrabromo-o-xylene, 97%
H-Pro-NMe2
2,4-DIMETHYL-3-HEPTANOL
[1-Hydroxy-3-(methylpentylamino)-propylidene]bisphosphonic acid sodium salt
Cefazolin sodium salt
AlbuMin bovine seruM;
Cineole
3-Chloro-1-phenyl-1-propyne
4-Methylbenzhydrol
5-(TrifluoroMethyl)picolinic acid
L-Iditol, 98+%
CADE OIL
Dibenzo[b,f][1,4]thiazepin-11(10H)-one
Iodothiophene; 95%
8-Fluoro-4-hydroxy-2-(trifluoromethyl)quinoline
2-AMINO-3-HYDROXY-2-METHYLBUTANOIC ACID
diazine black
3-Butyn-2-one
4-Butoxy-3-chlorophenylboronic acid
Tolylene 2,5-diisocyanate
3-BroMo-5-isopropoxyphenylboronic acid, pinacol ester
1-Methylpiperidine-4-carboxylic acid hydrochloride
Bindschedler's Green Leuco Base
Tris(3,5-dimethylphenyl)phosphine, 98%
3-(Cyclopentadienylpropyl)triethoxysilane (dimer)
1-Chloro-1-fluoroethane
4-Iodobenzyl bromide
5-AMINO-2-NITROBENZOIC ACID
1,2,3,4-Tetrachlorobenzene
3-(Phenylthio)thiophene
2,3,4-TriMethoxybenzaldehyde
5,5'-DibroMo-2,2'-bipyridyl
2,6-DiforMyl-3,5-diMethoxyphenol
TetraethylaMMoniuM p-toluenesulfonate, 98%
1,3-BUTADIENE
2-Ethoxyethyl Cyanoacetate
Neryl butyrate
3,6-Dihydroxynaphthalene-2,7-disulfonic acid, disodium salt
2,4-Hexadienyl acetate
5-Fluorotryptamine
EDN1 ELISA Kit
Cit ELISA Kit
Human insulin-like growth factors 2,IGF-2 ELISA Kit
Human NGF ELISA Kit
Lp-PL-A2 ELISA Kit
ALCAM ELISA Kit
PROG ELISA Kit
Human PAOX ELISA Kit
Human insulin-like growth factors 2,IGF-2 ELISA Kit
Mouse Collagen Type Ⅳ,Col Ⅳ ELISA Kit
B12 Assay Medium
Baird-Parker Agar Medium Base
ETHYLENE GLYCOL MONOSTEARATE
trans-1,2-Bis(tri-n-butylstannyl)ethylene, 96%
4-EthylphenylMagnesiuM broMide, 0.5M in 2-MeTHF
2-AMino-6-ethoxybenzothiazole, 97%
5-BROMOPYRIMIDIN-4-AMINE
2-(Diphenylphosphino)benzoic acid
N-(ETHOXYCARBONYL)-N-(ETHOXYCARBONYKLETHYL)GLYCINE ETHYL ESTER
Dicarbethoxydihydrocollidine
HAFNIUM TRIFLUOROACETYLACETONATE
4-HYDROXYBENZOIC ACID 4-METHOXYPHENYL ESTER
2-DiMethylaMinoethylaMine
trans-4-(BenzyloxycarbonylaMinoMethyl)cyclohexaneMethylaMine, 97%
2,6-DifluorobenzenesulfonaMide, 97%
Phenidone
IsoaMyl ether
1-(4-Fluorobenzoyl)pyrrolidine
4'-CYANOBENZYLIDENE-4-N-HEXYLOXYANILINE
Cyclohexane
AZURE A EOSINATE, PURE
2,2-Diethyl-1,3-propanediol
2-Furoic acid, 98%
3-Aminotetrahydrothiopene 1,1-dioxide hydrochloride , Tech.
2-Bromo-N-ethyl-4-(trifluoromethoxy)benzenesulphonamide