MA-c细胞株:小鼠星形胶质细胞系培养条件 货号:GD-C8618789
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞常规培养传代流程(请严格遵照无菌操作):1)吸出原培养瓶中的培养基,PBS缓冲液润洗细胞两次,加2-3ml 0.25%胰酶进行消化细胞(注意把握消化时间,通常控制在1-2min);2)镜下观察消化情况,在细胞边缘缩小,贴壁松动时(不建议消化到细胞漂浮)去掉胰酶,加6-8ml完全培养基,轻轻吹打细胞层,尽量把细胞层吹落,吹散;3)取部分细胞悬液转移到新的培养皿/瓶中,添加适当的完全培养基,把细胞悬液打匀,于培养箱中培养;4)注意培养基PH值变化情况,定期换液(每周2-3次),待细胞密度达到80%以后重复1项操作或者冻存;特别注意:(如使用公共实验室或者初次接触细胞培养,建议添加双抗培养)1)收到细胞后请尽快更换为含15%血清的新鲜培养基,如因特殊情况需要继续使用原瓶,请在原瓶培养基中额外添加10%的血清,(原瓶培养基的继续使用时间Z长不宜超过72小时);2)贴壁细胞收到当天切忌立刻消化,请将细胞换液后放置培养箱孵育到第二天再做消化传代,请优先选择直径6cm的培养皿进行传代培养;3)如签收时出现培养瓶壁破裂,漏液等情况请及时做好照片记录并联系实验室。
细胞培养实验中常见问题总结:1)一般客户拿到细胞后,应该注意什么?客户收到细胞后先不开盖,放在培养箱静置若干小时后(看细胞密度而定)在倒置显微镜下观察细胞生长情况,并对细胞进行不同倍数拍照(建议受收细胞后观察培养基的颜色和是否有漏液情况,显微镜下拍细胞100X,200X各一张),排除细胞本身污染的情况;收到细胞未开封,出现污染状况我们负责免费发送一株细胞。收到细胞时如无异常情况,请在显微镜下观察细胞密度,如为贴壁细胞,未超过80%汇合度时,将培养瓶中培养液吸出,留下10ml培养液继续培养;超过80%汇合度时,请按细胞培养条件传代培养。如为悬浮细胞,吸出培养液、1000转/分钟离心2分钟,吸出上清,管底细胞用新鲜培养基悬浮细胞后移回培养瓶。细胞消化液建议使用PBS配制,慎用Hanks液配制;2)快递细胞多久能到,是寄冻存的细胞还是复苏好的细胞?我们采用快递发货,一般外地2--3天,寄细胞前请确认当地温度,如果气温低于4度的,则采用邮寄冻存细胞;3)可否使用与原先培养条件不同之培养基?不能。每一细胞株均有其特定使用且已适应之细胞培养基,若骤然使用和原先提供之培养条件不同之培养基,细胞大都无法立即适应,造成细胞无法存活;4)可否使用与原先培养条件不同之血清种类?不能。血清是细胞培养上一个极为重要的营养来源,所以血清的种类和品质对于细胞的生长会产生极大的影响。来自不同物种的血清,在一些物质或分子的量或内容物上都有所不同,血清使用错误常会造成细胞无法存活。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2-AMino-6-ethoxybenzothiazole, 97%
Indoxyl acetate
3,5-Bis(trifluoroMethyl)benzoic acid
1-CHLORONONANE
4-AMINOCINNAMIC ACID
N-(2-AMinoethyl)-2-nitrobenzenesulfonaMide Hydrochloride , 98%
1-(4-FLUOROBENZYL)-4-(2-HYDROXYETHYL)
Dimethylvinylchlorosilane, 97%
2,3-Dichlorooctafluorobutane
5-Bromo-N-cyclopropylpyridine-2-carboxamide
(2-isopropoxyphenyl)amine(SALTDATA: HCl)
FMOC-L-BETA-HOMOLEUCINE
HEXAFLUORO-DL-VALINE
N-Boc-N'-benzyloxymethyl-L-histidine
11-Bromoundecanoic acid
L-(+)-Lysine Monohydrochloride
3-(DiMethylaMino)propionic Acid Hydrochloride
3-BroMo-4-(trifluoroMethoxy)benzonitrile
tert-Butylperoxy isopropyl carbonate, 75% solution in aroMatic free Mineral spirit
N-(1-Naphthyl)ethylenediaMine Dihydrochloride, Reagent, ACS
4-Fluoro-L-2-phenylglycine
ethyl benzenesulphonate
3,4-Dichlorobenzoylacetonitrile
methane-D3
3,4-Dimethoxybenzaldehyde
2,4,6-TRINITROBENZENESULFONIC ACID
Anisonitrile
4-BroMo-3-Hydroxybenzoic Acid
Copper iodate, anhydrous, 95%
3,5-Difluorobenzoic acid, 97%
Beta-Ionone
2,2-DiMethylbutyric Acid
NALPHA-(2,4-DINITROPHENYL)-L-ARGININE
SODIUM TETRADECYL SULFATE
Dodecylheptaglycol
6-Fluoroquinoline-8-boronic acid
5-Bromo-4-chloro-4,5,5-trifluoropentan-1-ol 97%
4-Dimethylaminopyridinium Bromide Perbromide
1-Methyl-1H-indazole-5-carbaldehyde
9,9-Bis(4-amino-3-chlorophenyl)fluorene
2-(TRIFLUOROMETHYL)-1,8-NAPHTHYRIDINE-3-CARBOXYLIC ACID
2,3,5,6-Tetrafluoroaniline
Glutaric acid dimethyl ester
Cyclohexanepropionic acid
Pipsyl chloride
Isovaleryl chloride
3-Hydroxy-2',4'-diMethyl-2-naphthanilide
(3S)-Boc-3-aMino-1-carboxyMethyl-caprolactaMe
3-IodothiobenzaMide, 98%
Spiramycin
1,1'-Di-n-octyl-4,4'-bipyridiniuM DibroMide
Trimethylsilyl acetate
3-Phenylbenzyl Bromide
N-Benzyl-1-aminoadamantane
xanthine monosodium salt
THULIUM 2,4-PENTANEDIONATE, TRIHYDRATE
3-METHOXY-5-METHYLPHENOL
1,3-Bis(aMinoMethyl)benzene
5-BroMo-4-p-tolylpyriMidine
3-(Perfluoro-3-methylbutyl)-1,2-propenoxide
5-Amino-2-pyridinol hydrochloride
(S)-1,2-Diaminopropane Dihydrochloride
p-(t-Butyl)phenethyltrichlorosilanecontains ~ 5% meta isomer
(S)-(+)-3-Hydroxytetrahydrofuran
1-Methyl-4-(methylsulfonyl)-benzene
Isoimperatorin
TetraMethylsilane;TMS
1-Butyl-3-MethyliMidazoliuM hexafluorophosphate
1,1'-Dibenzoylferrocene
2-BroMo-5-fluoropyridine-4-boronic acid
6-Hydroxyquinoline, 98%
L-Thyroxine sodiuM salt hydrate, 99%
2-AMinooctane
Heptacosane
2,4-Dichloroaniline
2-[(1R)-3-(dipropan-2-ylamino)-1-phenylpropyl]-4-(hydroxymethyl)phenol
Itraconazole
6-Bromovanillin
SAA ELISA Kit
IL-15 ELISA Kit
Human MANF ELISA Kit
Human CRT ELISA Kit
Chicken Acetoacetic acid,ACAC ELISA Kit
Rat CLEC2C (C-Type Lectin Domain Family 2 Member C) ELISA Kit
Monkey IgM (Immunoglobulin M) ELISA Kit
hs-CRP ELISA Kit
Human SEMA6C ELISA Kit
PRSS1 ELISA Kit
mEI Agar
Tetrathionate Broth Base
CadMiuM stearate
Silica
5-Fluoro-1H-indazole, 98%
2-Methylpyridine-4-carboxylic acid, 96+%
ethyl benzenesulphonate
Gelatin
Ampicillin trihydrate
Anthraquinone-2-sulfonic acid, sodium salt, monohydrate
Dichloroacetyl chloride
FORMOTHION
H-D-Phe-Otbu Hcl
2-n-Propylthiophene, 97%
DiisopentylaMine, 97%
1-VinyliMidazole
Ribonuclease A froM bovine pancreas
4-CHLORO-2-FLUOROBENZENESULFONYL CHLORIDE
(4R,5R)-2,2-DIMETHYL-1,3-DIOXOLANE-4,5-DICARBOXYLIC ACID DIMETHYL ESTER
5-BroMo-1-(4-Methoxybenzyl)pyrrole-2-carbaldehyde
1-CHLORO-3-FLUOROISOPROPANOL
2-Naphthaleneacetic Acid
Hederagenin
4-[(3-Chloro-2-methylphenyl)sulphamoyl]benzeneboronic acid
3-(2-piperidinyl)propanoic acid(SALTDATA: HCl)