SJCRH30[RC13,RMS 13,SJRH30]细胞：人横纹肌肉癌复苏细胞系更多折扣

SJCRH30[RC13,RMS 13,SJRH30]细胞：人横纹肌肉癌复苏细胞系更多折扣 货号：GD-C5920559
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
2-AMinobenzothiazole-6-carboxylic acid, 95%
5-Nitrothiophene-2-carboxylic acid
Ethylene carbonate
1H-Pyrazole-4-boronic acid
TRANS-5-DECEN-1-OL
2-Methoxycarbonyl-3-Methylpyridine-5-boronic acid pinacol ester
FMoc-Nalpha-Methyl-L-valine
2,8,9-Triisobutyl-2,5,8,9-tetraaza-1-phosphabicyclo[3.3.3]undecane, 97%
2-Chloro-6-nitrophenol, 98%
Dimethyl 2-Oxoglutarate
4-Bromo-N-tert-butylbenzenesulphonamide 98%
POLY(N-VINYLCARBAZOLE)
HEXAMETHONIUM BROMIDE
2-(DIFLUOROMETHOXY)BENZYL ALCOHOL
2,6-Dimethoxypyridine
4-BroMopyrazole
Dibutyltin Bis(trifluoroMethanesulfonate)
5-BroMo-2-(propylthio)pyriMidine
Squalene, 99+%
M-Cresol Acetate
5-Fluoro-2-pyridinecarboxylic Acid
2,2,2-Triphenylacetophenone
1-Dodecylimidazole
3-chloropropyl chloroformate
pyrrole
3-AMINO-4-METHYLBENZOTRIFLUORIDE
3-Amino-5-acetyliminodibenzyl
(R)-(-)-Epichlorohydrin
4,6-DibenzyloxypyriMidine, 95%
2-BroMo-4-fluorobenzyl broMide, 96%
3-Picoline
2-BroMo-3-hexylthiophene
1-Chlorododecane
tert-Butylbenzene
Food Yellow 3
3-(O-TOLYLOXY)PYRROLIDINE
6-Aminoindole ,98%
Z-Arg(Z)2-OH
2,6-Difluorobenzonitrile
2-Bromo-3-nitrotoluene, 98%
gamma-Decalactone
Sodium dehydrocholate
(S)-3-(4-HYDROXYPHENYL)-2-HYDROXYPROPIONIC ACID
4,4'-Cyclohexylidenebisphenol
BroMoforM (stabilized with Ethanol)
Osthole
TrichloroMethanesulfonyl Chloride
1-Pyrrolidinecarbonyl chloride, 99%
Bis(3,5,3',5'-diMethoxydibenzylideneacetone)palladiuM(0), 96%
m-Xylene
Naphthol AS-BI beta-D-Glucuronide [for BiocheMical Research]
2,3-Dichlorobenzoyl cyanide
3-Hydroxy-6,6-Dimethyl-1-Heptene-4-Yne
cis-3-hexenyl propionate
[[(chloromethyl)dimethylsilyl]methyl]trimethylsilane
D(+)-GLUCOSE MONOHYDRATE
2-(TrifluoroMethyl)pyridine-3-carbonitrile
5-Chloro-2-(trifluoroMethyl)benzaldehyde, 97%
2-AcetaMido-4-broMoanisole

3-(Hydroxymethyl)pyridine
Cyclopentadecanone Oxime

Furosemide
1-Aminonaphthalene-6-sulfonic acid
1-PHENYL-1,3,8-TRIAZASPIRO[4.5]DECAN-4-ONE
3-Methylthiophene
tert-Butyl 2-broMoisobutyrate
1-(2-Cyanoethyl)-2-MethyliMidazole
2-(4-(4,4,5,5-TetraMethyl-1,3,2-dioxaborolan-2-yl)-1h-pyrazol-1-yl)acetaMide
Isobutyric anhydride, 97%
Tetrabutyl orthosilicate, 95%

2-Chloro-5-nitrobenzonitrile
6-Amino-m-cresol
5-methyl-2-pyrrolidinone
Fmoc-Orn(Fmoc)-OH
1-Boc-2-Methyl
Insulin ELISA Kit
Mouse IL-25 (Interleukin 25) ELISA Kit
Human TNFSF13 ELISA Kit
Human SLA ELISA Kit
KLK3 ELISA Kit
CD151 ELISA Kit
Monkey α2-M (Alpha-2 Macroglobulin) ELISA Kit
Human RGC32 ELISA Kit
CT-1 ELISA Kit
NB-Ab ELISA Kit
Deoxycholate Hydrogen Sulfide Lactose Medium
Brucella Broth,Modified
CESIUM ORTHOVANADATE
Benzoyl peroxide
5-Methyl-1-hexyne, 98%
2-Methyltetrahydrofuran
2,2,2-Triphenylacetophenone
tripotassium citrate anhydrous
1,4-bis(methoxymethyl)-benzene
4,4'-Methylenebis(2,6-di-tert-butylphenol)

BISMUTH TITANATE
5-Chloro-2-Mercaptobenzothiazole
Sarcosine, 98%
2-Phenoxyaniline, 99%
2-(ChloroMethoxy)ethyltriMethylsilane, tech. 90%, stab. with 0.3% N-ethyldiisopropylaMine
TBTU
5-Methyl-2-propoxyphenylboronic acid
Poly(ethylene glycol)
1-Methyl-2-(tributylstannyl)-1H-pyrrole
4′-(DiMethylaMino)-2,2,2-trifluoroacetophenone
3-[(Benzyloxycarbonyl)aMino]propionaldehyde
2-Bromo-4-methyl-6-nitrophenol
methyl 6-amino-5-chloronicotinate
4-Methoxy-6-methyl-2-(methylsulfonyl)pyrimidine ,97%
[2-(Aminomethyl)phenyl]acetic Acid