HDF-a人成纤维细胞哪家有-成人

HDF-a人成纤维细胞-成人
货号：GD-C100218289
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞冻存的步骤：1)选择处于对数生长期的细胞，在冻存前一天Z好换液。将多个培养瓶中的细胞培养液去掉，用0.25% 胰蛋白酶消化。适时去掉胰蛋白酶，加入少量新培养液。用吸管吸取培养液反复吹打瓶壁上的细胞，使其成为均匀分散的细胞悬液。悬浮生产细胞则不要消化处理。然后将细胞收集于离心管中离心(1000r/min，10分钟)；2)去上清液，加入含20%小牛血清的完全培养基，于4℃预冷15分钟后，逐滴加入已无菌的DMSO或甘油，用吸管轻轻吹打使细胞均匀，细胞浓度为5×106～1×107/mL之间。(冻存培养液的配置是不是一定要用小牛血清呢？答案是不一定的，具体要看培养的细胞类型来选择；3)将分装上述细胞悬液于2ml冻存管中，每管0.25ml。冻存管要将盖子盖紧，并标记好细胞名称和冻存日期，同时作好登记(日期、细胞种类及代次、冻存支数)；4)将装好细胞的冻存管放到冻存盒中，-80℃冰箱过夜。；如果有程序降温器(放在-80℃冰箱过夜，放入液氮罐)Z好;或者可以在4℃，2h;然后转到-20℃，2h;-80℃，2h;放入液氮罐；5)细胞冻存在液氮中可以长期保存，但为妥善起见，冻存半年后，Z好取出一只冻存管细胞复苏培养，观察生长情况，然后再继续冻存。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
4-(4-Methyl-1-piperazinylcarbonyl)benzeneboronic acid hydrochloride, 95%
Gabapentin
1-Phenylsulfonylindole-6-boronic acid
CESIUM CHROMATE
1-Naphthyl phosphate calciuM salt trihydrate
4-BROMOCATECHOL
HOMO
5-Bromo-2-methoxyphenol
2'-Hydroxy-6'-methoxyacetophenone
3-[Thien-2-yl-5-(trifluoromethyl)]isoxazole
(R)-2-(Cyclohexylmethyl)succinic acid-1-methyl ester, 98% ee, 95%
gamma-Linolenic acid
3-Fluoro-4-methoxybenzeneboronic acid
2-HYDROXYETHYL BENZYL SULFIDE
2-[1-(Mercaptomethyl)cyclopropyl]acetic acid
2,4,5-TriMethoxybenzaldehyde
1-(DiMethylisopropylsilyl)iMidazole [DiMethylisopropylsilylating Agent]
3-BroMo-7-MethoxyiMidazo[1,2-a]pyridine-8-carbonitrile
Poly(propylene glycol), average M.W. 2.000
Ferrozine(R) , Iron Reagent
1-Hexyloxy-4-nitrobenzene
3-hydroxy-3'-nitro-2-naphthanilide
2-Chlorothiazole
1,1-Diphenylethylene
Tris(2-butoxyethyl) phosphate
2-Hydroxy-5-bromopyridine
TRANS-3-NONENE
5-AMino-2-Chlorobenzonitrile
IndiuM(I) broMide, Puratronic|r, 99.999% (Metals basis)
cis-4-(Boc-aMino)cyclohexaneMethylaMine, 97%
Barbital
Carbazole
PotassiuM (IodoMethyl)trifluoroborate , 98%
Zinc iodide
SILVER PHOSPHATE
1-Nitrobutane
Tetrabutylammonium fluoride 1.0M solution in THF
1,3-Bis(2,6-diisopropylphenyl)imidazolidin-2-ylidene
(5-Amino-1H-[1,2,4]triazol-3-yl)-methanol
5-Methoxy-2-methyl-3-indoleacetic acid, 98%
1-Methyl-1H-indole-3-carboxylic acid
3-Hydroxyphenylboronic acid
2-Amino-6-methoxypyridine
AMMONIUM PERCHLORATE
Mordant Yellow 10
1,3,5-Benzenetricarbonyl trichloride
2-Chloroethyl Laurate
2-(5-BroMothiophen-2-yl)
Ethyl propionylacetate, 98+%
TRH
4-Chlorobutyraldehyde DiMethyl Acetal
diethylmethoxyborane
Columbianetin acetate
N-Benzyl-2,4-Imidazolinedione
S-(4-methylphenyl) 4-methylbenzenesulfonothioate
4-FLUOROTHIOANISOLE
3-Pyridylacetic acid hydrochloride
2-(EthylaMinoMethyl)phenylboronic acid, pinacol ester
(DiMethylaMinoMethyl)phenol
3,4-Dichloro-5-nitrobenzotrifluoride
(5-Methylthien-2-yl)methylamine hydrochloride , 97%
Dipyridamole
4-[(2-Bromophenyl)carbamoyl]benzeneboronic acid 98%
SANTONIN
3-IODOPYRIDINE
L-Pyroglutamic acid
EcoR　I
Acetophenone
(+)-Biotin 4-Nitrophenyl Ester
4-Fluoro-3-(tetrahydro-2H-pyran-2-yloxy)phenylboronic acid
DiMethylsulfaMoyl chloride, 99%
1-(2-BroMophenyl)-1H-pyrazole, 97%
3,4-Diethylhexane
n-Octanoic Acid
Silver diethyldithiocarbamate
3-Bromoimidazo[1,2-a]pyridine-8-carboxylic acid
2-N-BUTYL-1,3-DIAZA-SPIRO[4,4]NON-1-EN-4-ONE
(S)-N-BOC-Allylglycine
TNOS ELISA Kit
Mouse C1qR1 (Complement Component 1Q Receptor1) ELISA Kit
Human CTSL3 ELISA Kit
Human BMP-10 (Bone Morphogenetic Protein 10) ELISA Kit
5NT ELISA Kit
MDA ELISA Kit
ANGPTL1 ELISA Kit
Galectin-9 ELISA Kit
CYP2E1 ELISA Kit
SFAS/Apo-1 ELISA Kit
Soya Casein Digest Lecithin Polysorbate Broth Base
Nitsch&Nitsch Medium
Murexide
Tragacanth powder
2-Benzyloxy-5-BroMo-6-Methylpyridine
2'-Hydroxychalcone, 98+%
3-hydroxy-3'-nitro-2-naphthanilide
Potassium tetraoxalate dihydrate
N-(4,6-dichloro-5-nitropyrimidin-2-yl)acetamide
Dichloroacetyl chloride

2-Bromomethyl-1,3-dioxolane
P-(METHYLTHIO)ISOBUTYROPHENONE
2-BroMo-4,6-Difluoroaniline
Benzyl acetate, 99%
3,5-DiMethoxyphenol, 98%
α-Chloralose
1,3-Bis(triphenylsilyl)benzene
1-Boc-3-piperidone
M-TOLUAMIDE
1-BOC-6-Methylindole-2-boronic acid
4-(ChloroMethyl)-1,2-diphenylethane, 98%
4-Bromo-2,6-dimethylphenyl isothiocyanate
Ammonium hydroxide, 25% solution in water, for analysis
N-Methyl-N-(3-nitrobenzyl)amine , 97%
1-Boc-5-aminoindazole