NCI-H28细胞系：人恶性间皮瘤细胞株 技术硬

NCI-H28细胞系：人恶性间皮瘤细胞株 技术硬 货号：GD-C3920214
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
细胞培养常见问题及解决方案:1)培养瓶有破裂，培养液有漏液：细胞极大可能会污染，所以我们会及时安排帮老师解决；2)细胞漂浮：培养瓶不开封，瓶口酒精擦拭后平躺放置在培养箱。次日观察，如细胞大部分又贴回瓶底，表明细胞活力正常，剩余漂浮的细胞可以离心去掉，留10ml培养液培养观察，细胞生长至汇合度80%，进行消化传代；如细胞还是不贴壁，将细胞离心收集转到新培养瓶，原培养瓶加部分培养液继续培养，中间注意观察，我们的技术人员会一直跟踪指导，直到问题解决；3)对于生长缓慢的贴壁细胞：可采用适当的提高培养基中血清浓度，或隔日换液的方法来保证细胞的状态和生长速度；4)对于生长不均的贴壁细胞：在培养过程中若出现细胞分布明显不均时(即某一区域细胞已重叠生长，而旁边则为一块空白)，此时可将细胞进行消化，重新打散，贴壁，加入新培养基进行培养。
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
传代培养及其操作步骤：原代细胞培养成功以后，需要进行分离培养，否则细胞会因生存空间不足或密度过大，营养障碍，影响细胞生长。细胞由原培养瓶内分离稀释后传到新的培养瓶中培养的过程称之为传代培养。传代细胞允许培养的细胞扩增(形成细胞株)，可以进行细胞克隆，易于保存，但可能丧失一些特殊的细胞和分化特征。传代细胞形成细胞株的利处在于提供了大量持久的实验材料，便于实验。【操作步骤】1)吸掉或倒掉培养瓶内旧培养液；2)向瓶内加入胰蛋白酶液和EDTA混合液少量。以能覆盖培养瓶底为宜；3)置37℃孵箱或室温(25℃温度)下进行消化，2~5min后把培养瓶放在倒置显微镜下进行观察，当发现胞质回缩、细胞间隙增大后，应立即中止消化；4)吸出消化液，向瓶内加入Hanks液少量，轻轻转动培养瓶，把残留消化液冲掉，然后再加培养液。如果仅使用胰蛋白酶消化，在吸除胰蛋白液后，可直接加入少量含血清的培养液，终止消化；5)使用弯头吸管，吸取瓶内培养液，按顺序反复轻轻吹打瓶壁细胞，使之从瓶壁脱离形成细胞悬液。吹打时动作要轻柔，以防用力过猛损伤细胞；6)用计数板计数后，分别接种于新的培养瓶中，置CO2培养箱中进行培养；7)细胞培养换液时间应根据细胞生长的状态和实验要求来确定。一般2～3d后应换一次生长液。待细胞铺满器皿底面，即可使用；也可继续传代扩大培养或换成维持液。【注意事项】1)掌握好细胞消化的时间，消化时间过短时，细胞不宜从瓶壁脱落，过长的消化会导致细胞脱落、损伤；2)掌握好消化浓度，当消化液浓度过高时，消化时间应缩短，过低时细胞消化时间相对延长。
2-DiMethylaMino-6-fluorobenzonitrile, 97%
Captan
2-BroMo-5-fluoro-4-forMylpyridine
5-BroMo-6-chloro-3-indolyl beta-D-Galactopyranoside (contains ca. 10% Ethyl Acetate) [for BiocheMical Research], 98%
Methyl 5-nitroisophthalate
3-ThiopheneMalonic acid
3-(1,1,2,2-Tetrafluoroethoxy)benzoic acid
4-Bromo-2-methoxypyridine
1,2-Phenylenediacetic acid, 99%
1-(10-Hydroxydecyl)imidazole
Monobutyl Itaconate
Acebutolol hydrochloride
N-METHYLBUTYLAMINE
5-CHLORO-2-HYDROXY-3-METHYLBENZALDEHYDE
Diethyl ethylMalonate
Isoquinoline-4-boronic acid 2,2-diMethylpropanediol-1,3 cyclic ester
3,5-Di-tert-butyltoluene
Ethyl 1-(4-broMophenyl)cyclopropanecarboxylate
Trifluoroacetic acid-d, for NMR, 99 atoM % D
PraMoxine Hydrochloride, USP
N-Acetyl-L-tyrosine Ethyl Ester Monohydrate
2,3,4-Trihydroxybenzoic acid
1-methylpiperidine-3-carboxylic acid
Phenylacetic hydrazide
1-bromo-10-fluorodecane
1,4-Diiodobutane
2-Chloro-5-Fluoroacetophenone
Tridecanal, 96%, stab.
N-Boc-L-^b-glutaMic acid 5-benzyl ester, 95%
2,3,5,6-TetraMethylbenzyl chloride, 98+%
Benzyl benzoate
2-BroMophenethylaMine
2,6-DiMethoxybenzylaMine
POTASSIUM 3-BROMOPHENYLTRIFLUOROBORATE
Iron(III) fluoride, anhydrous, 97% Min
Bis(aMinoMethyl)norbornane (Mixture of isoMers)
Butyl 2-chloroacetate , 97%
Methyl Fumaraldehydate
2-Mercapto-4,6-dimethylnicotinonitrile
cis,trans-5,9-Cyclododecadiene-cis-1,2-diol
N-Benzylacrylamide
10-NONADECANONE
2,3-DICHLOROANISOLE
Acetyl tributyl citrate
2,6-Difluorotoluene
1-Benzylpyrrole
1-(3-Chlorophenyl)pyrazole-4-boronic acid
CadMiuM standard solution, for AAS, 1 Mg/Ml Cd in 0.5N HNO3
7-BroMo-4H-1,3-benzodioxine, 95%
(S)-(4-Isopropyloxazolin-2-yl)ferrocene
2,4-Dinitrophenylhydrazine (contains HCl) Ethanol Solution [for TLC Stain]
2,6-dibromo-4-nitroaniline
2-Chloro-4-(trifluoromethyl)nicotinic acid
1-methyl-D-tryptophan
Fmoc-Lys(Boc)-OH
1,1,1,3,3,3-HEXAFLUOROISOPROPYL METHACRYLATE
BARIUM TARTRATE
2-(DI-T-BUTYLPHOSPHINO)-2'-METHYLBIPHENYL
6-Benzoyl-2-naphthol
1,1-BIS(3-CYCLOHEXYL-4-HYDROXYPHENYL)CYCLOHEXANE
Magnesium chloride, 97.5% (H2O - 2% max.)
Propionic Acid 1-Naphthyl Ester
Bis(trimethylsilyl)cytosine
1,1'-Diheptyl-4,4'-bipyridinium Dibromide [for Electrochromic Material]
2-Bromo-5-fluorobenzonitrile
ALLYL CROTONATE
BOC-D-4-THIAZOLYLALANINE
4-Nitrophthalic anhydride
1,8-Bis(diMethylaMino)naphthalene
DichlorodecylMethylsilane
1-(2-BroMophenylsulfonyl)-1H-iMidazole
SodiuM oxalate, 98.5%, extra pure
L-Tyrosine DisodiuM Salt
5-BroMo-6-chloro-3-indolyl beta-D-Galactopyranoside (contains ca. 10% Ethyl Acetate) [for BiocheMical Research]
4-Bromofluorobenzene
3-Methyl-2-picolinic acid
DL-Alanine ethyl ester hydrochloride
3-Picoline
ICAM-3 ELISA Kit
α-GST ELISA Kit
Human MUC7 ELISA Kit
Human pulmonary activation regulated chemokine,PARC ELISA Kit
Chicken toxoplasma circulating antigen,TCA ELISA Kit
S100A6 ELISA Kit
IL-4 ELISA Kit
Human TPPP ELISA Kit
VEGFR1 ELISA Kit
SOX1 ELISA Kit
CHO Medium Base
Columbia Agar Base
Octyl Isobutyrate
CALCIUM GLYCEROPHOSPHATE
5-Chloro-6-Hydroxy-3-Pyridinecarboxylic Acid
2-Fluorophenylhydrazine hydrochloride, 98%
2,3,4-Trihydroxybenzoic acid
Solvent Yellow 21
Tellurium(IV) iodide (99.9%-Te)
Nalidixic acid

2-(4-BROMOPHENYL)-5-(1-NAPHTHYL)-1,3,4-OXADIAZOLE
N-Carbobenzyloxy-L-aspartic acid
Phenethyl butyrate
CesiuM silicate (Meta), 99%
2-BroMo-2-nitropropane, 98%
4-Isopropylbenzenethiol
2-BroMo-5-fluorophenol
3-BroMo-2-chloro-5-(trifluoroMethyl)phenylboronic acid
2-Chloro-3-nitro-5-(trifluoroMethyl)pyridine
Pathalic acid
3-Isobutoxy-5-Methylphenylboronic acid, pinacol ester
4-Bromo-1H-pyrrole-2-carboxylic acid
Propyl Methanesulfonate
9-Borabicyclo[3.3.1]nonyl trifluoromethanesulfonate, 0.5M solution in hexanes, AcroSeal
threo-5,6-Dodecanediol