Roche试剂盒

产品应用：
    DIG-High Prime labeled probes can be used in Southern-, northern- and dot-blot analysis, as well as colony and plaque hybridizations. This kit offers random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and color detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering a ready-made blocking solution, combined stock solution of NBT/ BCIP, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.
产品介绍：
Sensitivity and specificity:The standard assay uses 1 µg of linearized pBR328. It is usual to expect 0.8 µg labeled DNA after 1 hour of labeling, and 2.3 µg labeled DNA after a 20-hour reaction. In a dot-blot hybridization, 0.1 pg of homologous DNA is detectable after a 16-hour color development (probe concentrations at 20 ng/ml). A single-copy human gene (tPA) is detectable in a Southern blot, loading 1 µg of digested placenta DNA.
组分：
 

1. DIG-High Prime, 5x conc., 50 µl
2. DIG-labeled Control DNA, 20 µl, (5 µg/ml), pBR328 (linearized with Bam HI)
3. DNA Dilution Buffer, 3 x 1 ml
4. Anti-digoxigenin-AP Conjugate, 100 µl
5. NBT/BCIP Stock Solution, conc., 6 x 1 ml
6. Blocking Solution, 10x conc., 4 x 100 ml
7. DIG Easy Hyb Granules, 4 x 100 ml

1. DIG-High Prime, 5x conc., 50 µl
2. DIG-labeled Control DNA, 20 µl, (5 µg/ml) pBR328 (linearized with Bam HI)
3. DNA Dilution Buffer, 3 x 1 ml
4. Anti-digoxigenin-AP Conjugate, 50 µl
5. CSPD, ready-to-use, 50 ml
6. Blocking Solution, 10x conc., 4 x 100 ml
7. DIG Easy Hyb Granules, 4 x 100 ml