重组人半胱氨酸蛋白酶YZ剂E/M蛋白

Cystatin E/M, also referred to as CST6, is a member of type 2 cysteine proteinase inhibitors of the cystatin superfamily. It inhibits papain and cathepsin B, but is not active against cathepsin C. In addition to being an inhibitor, cystatin E/M is also a substrate for transglutaminases. Cystatin E/M is required for viability and for correct formation of cornified layers in the epidermis and hair follicles,yet its expression and function may not be limited to cutaneous epithelia.Studies have revealed that the level of cystatin E/M appears to be significantly associated with primary pleural tumors.

·Description
Recombinant Human CystatinE/M produced by transiently transfected human cell is a secreted protein with sequence(Accesion#Q15828)(Arg29-Met149) of human CystatinE/M fused with a polyhistidine tag at the C-terminus.

·Quality Control

Biological activity:
The IC50 value of recombinant human CystatinE/M is approximately 7.0 nM, as measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC.
Purity: Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Amino-Acid Sequence: Predicted N terminal amino acid is Arg29.
Endotoxin:Less than 0.1ng/μg (1EU/μg) of rHu CystatinE/M.

·Formulation
Sterile and Lyophilized from a 0.2 μm filtered solution in PBS.

·Reconstitution
It is recommended to reconstitute the lyophilized rHu CystatinE/M in sterile water at no less than 100ug/mL,which can then be further diluted to other aqueous solutions.

·Storage
Lyophilized rHuCystatinE/M although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHu CystatinE/M should be stored at 4°C between 2-7 days and aliquots of reconstituted samples containing 0.1% BSA are stable up to 3 months below -18°C for future use.

Please avoid freeze-thaw cycles.