48T/96T盒 大鼠25羟基维生素D3(25检测试剂盒

大鼠25羟基维生素D3(25检测试剂盒双抗体夹心法检测抗原，间接法检测抗体，为客户提供各种ELISA技术进行样本检测。
英文缩写： 25(OH)D3/25 HVD3Elisa kit
规格96T/48T
保存条件：2-8℃低温保存
运输：快递运输（可根据客户要求，选择具体的运输方式，无特殊要求我公司一般为顺丰）。
大鼠25羟基维生素D3(25检测试剂盒样品准备:
1. Acidify the plasma/serum with an equal amount of buffer A. For example, if you are using 1ml of plasma/serum, add 1 ml of buffer A. Mix and centrifuge at 6,000 to 17,000 x g for 20 minutes at 4°C.
2. Equilibrate a SEP-COLUMN containing 200 mg of C18 (Catalog No.: RK-SEPCOL-1) by washing with buffer B (1 ml, once) followed by buffer A (3 ml, 3 times).NOTE: From steps 3-5, no pressure should be applied to the column.
3. Load the acidified plasma/serum solution onto the pre-treated (Step 2) C-18 SEP-COLUMN.
4. Slowly wash the column with buffer A (3 ml, twice) and discard the wash.
5. 大鼠25羟基维生素D3(25检测试剂盒Elute the peptide slowly with buffer B (3 ml, once) and collect eluant into a polystyrene tube.
6. Evaporate eluant to dryness in a centrifugal concentrator or by a suitable substitute method.
7(i). For EIA kits, Fluorescent EIA kits and Chemiluminescent EIA kits, keep the dried extract at -20°C and perform the assay as soon as possible. For normal subject extracted from 1 ml original plasma/serum, use 125 ul 1x assay buffer to reconstitute the dried extract. Aliquot 50 ul into two designated assay wells (25 ul is left over). the concentration factor in this case is 8. (1 ml / 125 ul = 8). The original plasma peptide level is 1/8 of the level of final extracted plasma.
大鼠25羟基维生素D3(25检测试剂盒样本要求:
在收集标本前都必须有一个完整的计划，必须清楚要检测的成份是否足够稳定。我们提倡新鲜标本尽早检测，对收集后当天就进行检测的标本，及时储存在4℃备用，如有特殊原因需要周期收集标本，请造模取材后，将标本及时分装后放在-20℃或-70℃条件下保存。因冰室与室温存在一定温差，蛋白极易降解，直接影响实验质量，所以避免反复冻融。代测放免标本的客户取材前须向我司销售人员索要说明书，具体操作注意事项请与我司技术人员沟通。
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