5-溴-4-氯-3-吲哚基磷酸酯对甲苯胺盐（BCIP) ,用于分子生物学, ≥99%_详细资料

产品信息 CAS号： 6578-06-9 供应商：江蓝纯数量： 99 英文名： 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt 保质期：两年规格： 100mg/500mg 规格： 100mg 产品价格： ¥695.0 规格： 500mg 产品价格： ¥2560.0 属性 敏感性对光线敏感，对空气敏感，对热敏感 熔点 196-198°C 存贮条件储存温度: -20°C,充氩保存 描述 产品介绍 For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indoly?l phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid. 别名 5-溴-4-氯-3-吲哚基磷酸酯对甲苯胺盐;X-phosphate p-toluidine salt 应用碱性磷酸酶(alkaline phosphatase)的底物。 5-溴-4-氯-3-吲哚磷酸(BCIP) 和氯化硝基四氮唑蓝(NBT)用于比色法鉴定碱性磷酸酶标记分子. BCIP/NBT 底物系统用途非常广泛：包括 Northern Southern, Western blotting, 原位杂交（in situ hybridization）和免疫组织化学。 BCIP 可以有两种盐的形式: 二钠盐型溶于水，对甲苯胺型溶于二甲基甲酰胺（DMF）。这些盐型可用于制备和NBT结合的储存溶液和反应缓冲液，构成碱性磷酸酶的底物溶液。底物系统和碱性磷酸酶一起培养，产生一种不溶的带紫色的NBT双甲腊( diformazan )产物，容易被观察。 温馨提示：不可用于临床ZL。

规格：	100mg	产品价格：	¥695.0
规格：	500mg	产品价格：	¥2560.0

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敏感性	对光线敏感，对空气敏感，对热敏感
熔点	196-198°C
存贮条件	储存温度: -20°C,充氩保存

产品介绍	For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indoly?l phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
别名	5-溴-4-氯-3-吲哚基磷酸酯对甲苯胺盐;X-phosphate p-toluidine salt
应用	碱性磷酸酶(alkaline phosphatase)的底物。 5-溴-4-氯-3-吲哚磷酸(BCIP) 和氯化硝基四氮唑蓝(NBT)用于比色法鉴定碱性磷酸酶标记分子. BCIP/NBT 底物系统用途非常广泛：包括 Northern Southern, Western blotting, 原位杂交（in situ hybridization）和免疫组织化学。 BCIP 可以有两种盐的形式: 二钠盐型溶于水，对甲苯胺型溶于二甲基甲酰胺（DMF）。这些盐型可用于制备和NBT结合的储存溶液和反应缓冲液，构成碱性磷酸酶的底物溶液。底物系统和碱性磷酸酶一起培养，产生一种不溶的带紫色的NBT双甲腊( diformazan )产物，容易被观察。