产品具体信息请参见:
http://www.yrgene.com/goods-123476.html货号:VYN0168
载体名称:pET-28a(+)
出品公司:Novagen
载体用途:原核表达载体
价格:900元规格:一管质粒,5ug。附送一管甘油菌,500ul。
到货周期:现货
载体别名:pET-28a(+)、pET-28a、pET28a(+)、pET28a、pET 28a
载体用途:原核表达载体
载体大小:5369bp
原核抗性:Kanamycin(卡那霉素)
启动子:T7 promter
表达水平:高
载体标签:N-His,N-Thrombin,N-T7,C-His
5' 测序引物:T7(通用测序引物,5'-TAATACGACTCACTATAGGG-3')
3' 测序引物:T7 ter(通用测序引物,5'-GCTAGTTATTGCTCAGCGG-3')
载体拷贝数:一般
备注:N端含有Thrombin蛋白酶切位点。pET28a,b,c的差异仅仅存在于多克隆位点处。
载体描述:The pET-28a-c(+) vectors carry an N-terminal His•Tag®/ thrombin/ T7•Tag® configuration plus an optional C-terminal His•Tag sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymeraseis shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single stranded sequencing should be performed using the T7 terminator primer.产品说明书及载体序列:请访问以下页面下载【pET-28a(+)】产品说明书及载体序列:
http://www.yrgene.com/goods-123476.html质粒图谱:
温馨提示:不可用于临床ZL。