现货，PD98059，selleck，美国进口， MEK YZ剂，CAS号: 167869-21-8，目录号 S1177_详细资料

产品信息

保存条件：零下20摄氏度低温保存数量：大量供应商： selleck中国广州办事处英文名： PD98059 CAS号： 167869-21-8 保质期：两年规格： 1 ml×5 /10mg

规格： 1 ml×5 产品价格：￥1323

规格： 10mg 产品价格：￥1323

S1177 PD98059
信号转导通路: MAPK >> MEK >> MEK YZ剂 >> PD98059
技术数据:

分子量(MW): 267.28

化学式:
C₁₆H₁₃NO₃

溶解度: DMSO ≥32mg/mL Water <1mg/mL Ethanol ≥3mg/mL

纯度: >99%

稳定性: at -20℃ 2 years

CAS号: 167869-21-8

生物活性

PD 98059 is a highly selective inhibitor of MEK1 and MEK2 with IC50 values of 4 µM and 50 µM respectively^[1–3] . PD98059 does not inhibit activation of other highly related dual-specificity protein kinases or the activity of over 18 other Ser/Thr protein kinases. At concentrations up to 100 µM, PD98059 does not inhibit activation of MKK3 or SEK (MKK4) as determined by measuring phosphorylation at its activation site. ^[1–3]

参考文献

Regional Selective Neuronal Degeneration after Protein Phosphatase Inhibition in Hippocampal Slice Cultures: Evidence for a MAP Kinase-Dependent Mechanism Elise Runden,Per O. Seglen,et al. The Journal of Neuroscience September 15, 1998;18(18):7296–7305

Mitogen-Activated Protein Kinases (Erk1,2) Phosphorylate Lys-Ser-Pro (KSP) Repeats in Neuroﬁlament Proteins NF-H and NF-M Veeranna,Niranjana D. Amin,et al. The Journal of Neuroscience June 1, 1998;18(11):4008–4021

Nerve Growth Factor Activates Extracellular Signal-Regulated Kinase and p38 Mitogen-Activated Protein Kinase Pathways To Stimulate CREB Serine 133 Phosphorylation JUN XING,JON M. KORNHAUSER,et al. MOLECULAR AND CELLULAR BIOLOGY Apr. 1998;18:1946–1955

客户反馈数据

T47D cells were pretreated with 100ng/ml EGF for 15 min and then treated with the indicated concentrations of PD98059 for 24 hours.

effect of ERK1/2 inhibitor PD98059 (1, 5, and 10 M) on SRP72 protein expression was evaluated on Jurkat cells stimulated with rhIL-1. The cells were harvested at 0,120, and 240 min, and the protein expression was verified byWBusing antibodies against human SRP72, human SRP54 (nonphosphorylated SRP protein), and humanGAPDHas constitutive protein.Adecreased SRP72 expression at 10M and 240 min was found.

C, effect of ERK1/2 inhibitor PD98059 (1, 5, and 10 M) was evaluated in SRP72-immunoprecipitated cell lysates. WB using anti-phosphoserine antibody showed a decreased SRP72 band when used as the inhibitor at concentrations of 1 M at 240 min, 5 M at 120 min, and 10 M at 120 and 240 min (lanes 3, 5, 8, and 9). D, results were analyzed and RUA illustrated, finding significant results at 1 M at 240 versus 0, 5 M at 120 versus 0, and 10 M at 120 versus 0 min.

We used real time RT-PCR to investigate the effects of PD98059 on SRP72 mRNA expression. All the samples were treated under the same experimental conditions used in protein tests done by WB, and phosphorylation was measured indirectly by IP-WB. The results did not show significant changes on the mRNA expression of SRP72 after the treatment with these inhibitors.

如果需要长期保存，请于零下二十度低温保存。

禁止用于人体及ZL！

特定的存储和包装每个产品的信息在产品说明书上都有注明。大多数Selleck产品，在推荐的条件下存储可稳定保存两年。产品有时建议的储存温度不同，大多数建议储存在-20 ° C ，抗体及蛋白等产品建议-60℃。YZ剂属于化学试剂，可在常温下运输储存两周左右。即使如此，我们保证产品的出货量将保持产品质量的条件下，一般都会放入冰袋。望阁下收到产品后，请按照产品数据表建议适当存储。

温馨提示：不可用于临床ZL。

规格：	1 ml×5	产品价格：	￥1323
规格：	10mg	产品价格：	￥1323

分子量(MW):	267.28
化学式:	C₁₆H₁₃NO₃
溶解度:	DMSO ≥32mg/mL Water <1mg/mL Ethanol ≥3mg/mL
纯度:	>99%
稳定性:	at -20℃ 2 years
CAS号:	167869-21-8

	T47D cells were pretreated with 100ng/ml EGF for 15 min and then treated with the indicated concentrations of PD98059 for 24 hours.
	effect of ERK1/2 inhibitor PD98059 (1, 5, and 10 M) on SRP72 protein expression was evaluated on Jurkat cells stimulated with rhIL-1. The cells were harvested at 0,120, and 240 min, and the protein expression was verified byWBusing antibodies against human SRP72, human SRP54 (nonphosphorylated SRP protein), and humanGAPDHas constitutive protein.Adecreased SRP72 expression at 10M and 240 min was found.
	C, effect of ERK1/2 inhibitor PD98059 (1, 5, and 10 M) was evaluated in SRP72-immunoprecipitated cell lysates. WB using anti-phosphoserine antibody showed a decreased SRP72 band when used as the inhibitor at concentrations of 1 M at 240 min, 5 M at 120 min, and 10 M at 120 and 240 min (lanes 3, 5, 8, and 9). D, results were analyzed and RUA illustrated, finding significant results at 1 M at 240 versus 0, 5 M at 120 versus 0, and 10 M at 120 versus 0 min.
	We used real time RT-PCR to investigate the effects of PD98059 on SRP72 mRNA expression. All the samples were treated under the same experimental conditions used in protein tests done by WB, and phosphorylation was measured indirectly by IP-WB. The results did not show significant changes on the mRNA expression of SRP72 after the treatment with these inhibitors.

您可能感兴趣的产品

现货，PD98059，selleck，美国进口， MEK YZ剂，CAS号: 167869-21-8，目录号 S1177

产品信息

联系我们

关注我们