罗氏Complete蛋白酶YZ剂:
有了Complete蛋白酶YZ剂混合片,您便不用再花时间去寻找合适的YZ剂,只要一片complete溶入缓冲液,便可GXYZ多种不同蛋白酶YZ剂。
水溶型片剂,无需繁琐的称量,混合以及DMSO溶解,使用安全方便。
新型:cOmplete ULTRA Tablets, Mini, EASYpack
cOmplete ULTRA Tablets, Mini, EDTA-free, EASYpack
新式蛋白酶YZ剂可以完全YZ天冬氨酸(新增加),丝氨酸,半胱氨酸,金属蛋白酶等的活性,适用于细菌,酵母,昆虫,哺乳动物细胞等几乎所有细胞类型的样本。
货号 | 货名 | 规格 | 价格 |
05892970001 | cOmplete ULTRA Tablets, Mini, EASYpack | 30 tablets | 2470 |
05892791001 | cOmplete ULTRA Tablets, Mini, EDTA-free, EASYpack | 30 tablets | 2470 |
产品应用:
During cell lysis and purification, target proteins are at risk of being cleaved by proteases, which are naturally present in cells. To ensure that full-length proteins are obtained, cOmplete ULTRA Tablets are used for the inhibition of an extended range of proteases. This new formulation shows high inhibition ratios due to its broadened range of inhibitors compared to standard cOmplete Tablets.
This new protease inhibitor cocktail now inhibits aspartic proteases, as well as serine and cysteine proteases. cOmplete ULTRA Tablets were developed for improved efficiency on different cell types. Best performance is guaranteed in lysates of bacteria, yeast, insect cells, and a wide range of higher cell lines.
This tablet does not contain EDTA; therefore, metalloproteases are not inhibited.
For the purification of histidine-tagged proteins using Immobilized Metal Ion Affinity Chromatography (IMAC), this EDTA-free tablet is recommended for applications using non-EDTA compatible IMAC resins. Alternatively, an EDTA-containing cOmplete ULTRA, Mini tablet may be used in combination with Roche’s new EDTA-compatible chromatography material, cOmplete His-Tag Purification Resin.
优势:
Complete protection
The extended inhibitor cocktail composition makes this tablet an ideal formulation for your most difficult, protease-sensitive targets.
Convenient tablet format
Eliminate weighing small amounts, and instantly protect your proteins against a now even wider range of proteases by simply pushing a tablet through the EASYpack foil packaging directly into your buffers.
Suitable for IMAC purification
Purify poly-histidine tagged proteins using resins that are not compatible with EDTA. With Roche’s new EDTA-compatible cOmplete His-Tag Purification Resin, a tablet with EDTA may be used in IMAC, offering significant better protection of your target protein even against metalloproteases.
产品介绍:
cOmplete ULTRA Tablets, Mini, EDTA-free contain the most powerful protease inhibitor cocktail for the optimal protection of your proteins against protease degradation. They are supplied as tablets in an EASYpack blister pack. EASYpacks provide the most convenient form of delivery - single tablets are easily pushed through the blister foil directly into the buffer. One cOmplete ULTRA Tablet, Mini, EDTA-free is sufficient for a volume of 10 ml extraction solution.
背景信息:
Recombinant proteins may be partially degraded during expression. Soluble degradation products are co-purified and cause a non-homogeneous protein preparation with protein fragments of different lengths. However, metalloproteases are prevalent in, for example, bacteria, and some techniques require that EDTA be omitted.
Obtain convenient, reliable protection of your proteins against dephosphorylation using the new PhosSTOP Phosphatase Inhibitor Cocktail Tablets. You can easily inhibit both proteases and phosphatases at the same time by simply combining a cOmplete ULTRA, Mini, Protease Inhibitor Cocktail Tablet with a PhosSTOP Phosphatase Inhibitor Cocktail Tablet in the same 10 ml buffer.
组成:
30 tablets individually packed in foil blister packs. Each tablet is sufficient for a volume of 10 ml extraction solution.
产品应用:靶蛋白在细胞裂解和纯化,裂解蛋白酶,它们在细胞中自然存在的风险。为了确保获得全长蛋白,完整的超片用于YZ蛋白酶的扩展范围。这一新的提法,显示了极高的YZ率,由于其扩大范围YZ剂相比标准的完整片。现在这种新的蛋白酶YZ剂的鸡尾酒YZ的天冬氨酸蛋白酶,以及丝氨酸和半胱氨酸蛋白酶。超完整的片剂,开发了不同类型的细胞提GX率。的性能保证在细菌,酵母,昆虫细胞,广泛和较高的细胞株的细胞裂解液。这种平板不包含EDTA的,因此,金属蛋白酶没有YZ。纯化组氨酸标签的蛋白质,利用固定化金属离子亲和层析(IMAC),EDTA无平板推荐使用非EDTA兼容的IMAC树脂的应用。另外,含EDTA -完整的超迷你平板可结合使用,与罗氏公司的新EDTA兼容色谱材料,完成His - tag的纯化树脂。
优势:延长YZ剂的鸡尾酒组成完整的保护,使这片Z困难的,蛋白酶敏感目标的理想配方。便民平板格式消除重少量,瞬间保护您的蛋白质直接进入缓冲区,只需简单地推通过EASYpack铝箔包装片剂对现在甚至更广泛的蛋白酶。适合IMAC纯化净化聚组氨酸标签使用的树脂,用EDTA不兼容的蛋白质。与罗氏公司的新EDTA兼容完成他的标签纯化树脂,可用于iMac电脑,提供更好地保护您的目标蛋白质即使对金属蛋白酶与EDTA的平板。
产品介绍:超完整片,迷你,EDTA无包含您对蛋白酶降解蛋白质的保护大的蛋白酶YZ剂的鸡尾酒。他们提供的EASYpack泡罩包装的药片。 EASYpacks提供Z方便的形式交付 - 单粒很容易直接通过泡罩铝箔推入缓冲区。一个完整的超片,迷你,EDTA无足够量为10毫升提取液。
背景信息:表达重组蛋白的过程中可能会部分降解。可溶性降解产物共同纯化,并导致非均质的蛋白制备具有不同长度的蛋白质碎片。然而,金属蛋白酶是普遍存在的,例如,细菌,和一些技术要求EDTA的省略。对使用新PhosSTOP磷酸酶YZ剂片的去磷酸化,获取方便,可靠的保护您的蛋白质。通过简单的相结合,迷你,一个完整的超蛋白酶YZ剂鸡尾酒一个PhosSTOP磷酸酶YZ剂在相同的10毫升缓冲鸡尾酒平板平板,您可以轻松地同时YZ蛋白酶和磷酸酶。
组成:30片单独包装铝箔泡罩包装。每片10毫升提取液的体积是足够的。
罗氏现货产品:转染试剂(现货): 11814443001 FuGENE 6 Transf. Reag. 4709705001 FuGENE HD Transfection Reagent 6365787001 X-tremeGENE 9 DNA Transf. Reag. 6366236001 X-tremeGENE HP DNA Transf. Reag 蛋白酶以及磷酸酶YZ剂(现货):4693116001 cOmplete,EASYpack,20 Tab.4693132001 cOmplete,EDTA-free,EASYpack,205892791001 cOmplete ULTRA Tablets, Mini, EDTA-free, EASYpack5892970001 cOmplete ULTRA Tablets, Mini, EASYpack4906845001 PhosStop, 10 Tablets4906837001 PhosStop, 20 Tablets
温馨提示:不可用于临床ZL。