2′-脱氧胞苷 5′-三磷酸 二钠盐

Application

Use dCTP, lithium salt, for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions.

2′-Deoxycytidine 5′-triphosphate disodium salt is one of the nucleoside triphosphates used for DNA polymerase driven reactions such as polymerase chain reaction, DNA sequencing and other molecular biology methods.

General description

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dCTP is a 100 mM clear, colorless solution of the lithium salt (pH 7).
dCTP solution in water (pH 7), 100 mM, lithium salt.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Our PCR Grade deoxynucleotides are even purer than the lithium salts, to ensure excellent performance in your PCRs. PCR Grade deoxynucleotides contain the sodium salt of the nucleotide in PCR Grade Water.
For your convenience, some of our deoxynucleotides are also available in:
• nucleotide sets (all 4 nucleotides in separate vials)
• ready-to-use nucleotide mixes (premixed solutions of all 4 nucleotides)

They are also vital components of:
• kits (dNTPs, buffer, and polymerase in separate vials)
• ready-to-use mixes [polymerase, nucleotides, and buffer (premixed 2x solutions)] 

Quality

Typical analysis:
• ≥98% dCTP (HPLC), ≤1.5% dCDP (HPLC), ≤0.5% dCMP (HPLC)
• Absorbance determination A₂₅₀/A₂₆₀, A₂₈₀/A₂₆₀, A₂₉₀/A₂₆₀
• DNase activity negative, according to the current Quality Control procedures
• Suitability for PCR 

Unit Definition

1 μmol dCTP = 9.6 A₂₇₂ units