Species | Human |
Accession | P01579 |
GeneID | 3458 |
Source | Escherichia coli. |
Molecular Weight | Approximately 16.9 kDa, a single non-glycosylated polypeptide chain containing 144 amino acids. |
Quantity | 20µg/100µg/1000µg |
AA Sequence | MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRGR RASQ |
Purity | > 98 % by SDS-PAGE and HPLC analyses. |
Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using HT-29 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107 IU/mg. |
Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation | Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. |
Endotoxin | Less than 1 EU/µg of rHuIFN-γ as determined by LAL method. |
Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
Usage | This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. 我公司销售各种重组人细胞包括:β细胞素,人骨成型蛋白系列,人脑钠素,重组人头蛋白。等 |