产品具体信息请参见:
http://www.yrgene.com/goods-122973.html
货号:VPI0008
载体名称:pcDNA3.1(hyg)+ / pCDNA3.1-Hygro(+) / pcDNA3.1/Hygro(+)
出品公司:Invitrogen
载体用途:哺乳动物表达载体
价格:900元
规格:一管质粒,5ug。附送一管甘油菌,500ul。
到货周期:现货
原核抗性:Ampicillin(氨苄青霉素)
真核筛选标记:Hygromycin(潮霉素)
启动子:CMV promter
表达水平:高
5' 测序引物:CMV-f
3' 测序引物:BGH-r
载体拷贝数:高
载体描述:
This pcDNA3.1/Hygro(+) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Hygromycin selectable marker and a reverse-orientation multiple cloning site.
The pcDNA™3.1 Expression Vector Family
Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin®, Zeocin™, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features:
- Cytomegalovirus (CMV) enhancer-promoter for high-level expression
- Large multiple cloning site in either forward (+) or reverse (-) orientations
- Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence for enhanced mRNA stability
- SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (i.e., COS-1 and COS-7)
- Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli
产品说明书及载体序列:
请访问以下页面下载【pcDNA3.1(hyg)+ / pCDNA3.1-Hygro(+) / pcDNA3.1/Hygro(+)】产品说明书及载体序列:
http://www.yrgene.com/goods-122973.html
质粒图谱:
温馨提示:不可用于临床ZL。