磷酸化丝氨酸/苏氨酸/酪氨酸抗体供应,应用于IHC、WB、 IF、IP、ELISA等实验,公司生产的抗体每个流程都执行严格的检测标准,保证蛋白抗原产品质量,质量稳定,实验效果明显。按理化性质和生物学功能,可将其分为IgM、IgG、IgA、IgE、IgD五类。公司产品经无数次市场验证,若出
供应:Phosphoserine/threonine/tyrosine抗体,磷酸化丝氨酸/苏氨酸/酪氨酸抗体促销供应,应用于IHC、WB、 IF、IP、ELISA等实验,质量保证,无效免费退换,另外本公司生产经营各种标记二抗、免疫印迹常用试剂、免疫组化常用试剂,欢迎来电咨询!
抗体名称:
Phosphoserine/threonine/tyrosine抗体,磷酸化丝氨酸/苏氨酸/酪氨酸抗体
规格价格: 0.1ml/100ug 0.2ml/200ug
说 明 书:电询
研究领域:肿瘤 细胞生物 免疫学 信号转导 转录调节因子
抗体来源:Rabbit(兔) 小鼠
克隆类型:Polyclonal 单抗
交叉反应:Human, Mouse, Rat, Chicken, Pig, Cow, Horse,
产品应用:WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Lyophilized or Liquid
浓 度:1mg/1ml
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
保存条件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.贮存于-20℃.
Phosphoserine/threonine/tyrosine抗体,磷酸化丝氨酸/苏氨酸/酪氨酸抗体Product characteristics dilution method:Methods 1. Before the experiment, the frozen antibodies dry with sterile distilled water dilution (or PBS diluted), will be diluted antibody packaging 5-10 branch (20ulx5 branch or 10ulx10 branches and placed in - 20 DEG C to save, a take a, avoid repeated freezing and thawing.
Method 2. Before the experiment, but also will freeze antibodies dry with sterile distilled water dilution 50ul (or PBS dilute 50ul) into solution: plus 50% glycerol, placed in - 20 DEG C to save, how much ceiling.
Methods 3 pre test can do a few of the gradient 1:100, 200, 400 background can also be done to go up, choose a best dilution ratio, and then do the best effect. Dilution of working fluid - using antibody dilution.
Each process of the production of antibodies to perform strict testing standards, to ensure the quality of protein antigen products, quality and stability, the experimental effect is obvious. Physicochemical properties and biological function, which can be divided into IgM, IgG, IgA, IgE, IgD five.
Comparison of paraffin section and frozen section
(1) it is not necessarily the one that makes the frozen sections of a frozen section, which is the conclusion I have drawn to a teacher today. Because the, will destroy the tissue antigens of paraffin sections were made to high temperature baking sheet, if tissue antigenicity is stable, paraffin sections were made; but which require paraffin and frozen section was made.
(2) the frozen section has the advantage of being able to preserve the tissue antigen immune activity, and it is not necessary to fix the antigen in the immune system. The disadvantage is that it is easy to form ice crystals in the cell and damage the cell structure, which may cause the antigen to diffuse; the thickness of the slice is thicker than that of paraffin, and the film is not so good. When you buy an anti -, the directory is written to do what kind of slice, if it is written to be frozen, it can not be done, such as the two can be, it can do.
(3) paraffin sections of the advantages of can maintain the morphology and structure of the tissue cells and easy to store at room temperature, and frozen section is too much trouble, must exist to 80 degrees of the cryogenic refrigerator, especially used for in situ hybridization of sections, in order to prevent RNA degradation and preservation has always been very important. As the paraffin sections can be cut to about 4 microns, so in situ hybridization probe easy to penetrate into the tissue, easy to succeed, and get the color / shape is better than frozen section.
Phosphoserine/threonine/tyrosine抗体,磷酸化丝氨酸/苏氨酸/酪氨酸抗体产品经无数次市场验证,欢迎来电咨询,竭诚为您推荐下列优质系列产品:
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Animal serum series:
Fetal bovine serum, newborn calf serum, newborn calf serum, newborn bovine serum, standard, standard horse serum, super horse serum, goat serum, sheep serum, rabbit serum, chicken serum, swine serum, bovine serum, calf serum, dog serum, Donkey Serum, rat serum, serum, guinea pig serum, Babixi in serum.
Plasma series:
Fetal bovine serum, newborn bovine serum, Daniel plasma, bovine plasma, rabbit plasma, plasma of horses, goat plasma, plasma of sheep, chicken plasma, porcine plasma, dog plasma, donkey plasma, rat plasma, mouse plasma and plasma of guinea pig.
The whole blood series (glass bottle and Pet plastic bottle):
Newborn bovine blood, the blood of goats, sheep blood, blood horse, donkey blood, blood of rabbit, rabbit red blood cells (20%), sheep red blood cells (20%), chicken red blood cells (20%), rabbit red blood cells (4%), 4% of sheep red blood cells chicken red blood cell, (4%).
The whole blood series:
The new blood, the blood of goats, sheep blood, blood horse, donkey blood
The splitting blood (defiber cracking, anticoagulant cracking)
Take off the fiber and the blood: the calf blood, the blood of the bull and the goat blood, the blood of the sheep, the horse blood, and the blood of the donkey.