is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K remains active:
• Over a wide pH range (optimal activity between 6.5 and 9.5)
• Under denaturing conditions (e.g., in the presence of SDS or urea)
• In the presence of metal chelating agents (e.g., EDTA)
• At comparatively high temperatures (optimum digestion temperature is 65°C)
ApplicationsRemoval of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for
in situ hybridization.
Performance and quality testingEndodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.
Unit definitionOne mAnson unit is described as that amount of enzyme that liberates 1 µmole of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.
规格
Form: | Liquid |
Species: | T. album |
Product Size: | 5 x 1.25 mL |
Shipping Condition: | Dry Ice |
内容及储存
Store at –20°C.
25530015 | 蛋白酶K 100 MG | 1604 |
AM2548 | 蛋白酶 K (20MG/ML)5X1.25ML EACH | 2455 |