TaqMan® Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR
TaqMan® Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR). You can use TaqMan® Universal Master Mix II to amplify complementary DNA (cDNA) and DNA targets for a variety of applications, including quantitation and genotyping.
This TaqMan® Universal Master Mix II is supplied as a 2X concentrate and contains:
• AmpliTaq Gold® DNA Polymerase, UP (Ultra Pure)
• dNTPs (with dUTP)
• ROX™ Passive Reference
• Uracil-N glycosylase (UNG)
• Optimized buffer components
Note: This TaqMan® Universal Master Mix II contains Uracil-N glycoslyase (UNG). See a complete listing of all TaqMan® Universal Master Mix II products, with and without UNG here.
TaqMan® Universal Master Mix II has been optimized for use with primers and TaqMan® probes that have been designed according to Life Technologies' guidelines. The master mix can be used with custom TaqMan® assays available from our custom assay service, or with pre-optimized assays such as:
• TaqMan® Gene Expression Assays
• TaqMan® MicroRNA Assays
• TaqMan® Drug Metabolism Genotyping Assays
• TaqMan® SNP Genotyping Assays
For RNA quantitation experiments, the TaqMan® Universal Master Mix II is used in the second step of a two-step reverse transcription/polymerase chain reaction (RT-PCR) protocol. The cDNA template used with the master mix can be generated in a reverse transcription reaction using cDNA synthesis kits
available from Life Technologies.
规格
Detection Method: | Primer-Probe Detection |
Platform: | 7300 System, 7500 System, StepOnePlus™, Standard Mode, ViiA™ 7 System, StepOne™, Standard Mode, 7900HT System |
Reaction Speed: | Standard |
PCR Method: | qPCR |
Sample Type (General): | DNA |
Polymerase: | AmpliTaq Gold® DNA Polymerase |
Product Size: | 1 x 5 mL |
Shipping Condition: | Wet Ice |
Passive Reference Dye: | ROX (Pre-mixed) |
Sample Type (Specific): | cDNA, DNA (Genomic) |
Purity or Quality Grade: | UP (Ultra Pure) |
Primer-Probe Compatibility: | TaqMan® |
内容及储存
Store at 4°C
Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
4440038 | TaqMan® Universal Master Mix II, with UNG 1-Pack (1 x 5 mL) | 200 rxn | 5340 |
4440040 | TaqMan® Universal Master Mix II, no UNG 1-Pack (1 x 5 mL) | 200 rxn | 4959 |
4440042 | TaqMan® Universal Master Mix II, with UNG Mini-Pack (1 x 1 mL) | 100 rxn | 1122 |
4440043 | TaqMan® Universal Master Mix II, no UNG Mini-Pack (1 x 1 mL) | 100 rxn | 1122 |
4440048 | TaqMan® Universal Master Mix II, no UNG 5-Pack (5 x 5mL) | 2500 rxn | 22050 |
4440045 | TaqMan® Universal Master Mix II, with UNG 5-Pack (5 x 5mL) | 2500 rxn | 23360 |