Human Eosinophil cationic protein（ECP） ELISA Kit说明书_详细资料

产品信息

Human Eosinophil cationic protein（ECP） ELISA Kit说明书英文名称：产品类别：Humam ELISA Kit商品品牌：SANCHEZ C AS 号：eia06538h分子式：分子量：包装类型：盒装产品库存：100[批发价格]规格价格纯度96T电议

Cat No.eia06538h
Human Eosinophil cationic protein(ECP) ELISA Kit
Dear customers, thank you for choosing our products. This product is suitable for in vitro qualitative detection of human serum, plasma or cell culture supernatant ａnd organizations in the natural ａnd recombinant ECP concentration. Detection of other special sample please contact our technical support. The kit is for research use only. Please read the instructions carefully before using ａnd check the kit components. If you have any questions, please contact SANCHEZ INC You will get our full range of services.

This kit employs Double Antibody Sandwich Technique. The principle of Double Antibody Sandwich is based on characteristics of the tested antigen with more than two valances which can identify coated antibody and detection antibody at same time. The specific process is as follows:

1. Connect the specific antibodies ａnd solid phase carriers to form immobilized antibodies. Wash out uncombined antibodies ａnd impurities. Seal the rest binding sites with irrelevant proteins.
2. Join under test with immobilized antibodies for contact reaction. After a while, combine antigens in ａnd antibodies on carriers into the antigens complex. Wash out uncombined antibodies ａnd impurities.
3. Add biotin labeling antibodies to combine with the antigens on immune complexes. Wash out the uncombined biotin labeling antibodies thoroughly. The enzyme amount on the carrier is now positively related to the amount of the tested substance in specimens.
4. Add horseradish peroxidase to label the avidins ａnd incorporate them with the biotin labeling antibodies. Wash out the incorporated enzyme markers thoroughly. The enzyme amount on the carrier is now positively related to the amount of the tested substance in specimens.
5. Add substrates for coloring, ａnd compute the concentration of specimens.
Note: an antibody molecule can be marked on several biotin molecules ａnd a biotin molecule can be connected with a HRP-Avidin to form numbers of horseradish peroxidases combining with antibodies which shows higher sensitivity ａnd amplification effect comparing with traditional direct HRP-Antibodies.

【Detection principle of Human eosinophil cationic protein(ECP) ELISA kit】
This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human ECP monoclonal antibody ａnd the detecting antibody is polyclonal antibody with biotin labeled. Samples ａnd biotin labeling antibody are added into ELISA plate wells ａnd washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic ａnd finally turns into yellow under the action of acid. The color depth ａnd the testing factors in samples are positively correlated.
【Kit composition】
name                    96 Tests  48 TestsStorage
1．antibody precoated plate    8×12      8×64/-20℃
2．Human ECP Standards   2 vial      1 vial4/-20℃
3．Biotinylated antibody(1:100)    2 vial     1 vial4/-20℃
4．Enzyme conjugate(1:100) 1vial      1 vial4/-20℃
5．Enzyme diluent                1vial  1vial        4/-20℃
6．antibody diluent               1vial  1 vial4/-20℃
7．Standard diluent               1vial 1 vial      4/-20℃
8．Sample diluent                1vial  1 vial   4/-20℃
9．Washing buffer (1:25)         1 vial 1 vial    4/-20℃
10．Colour Reagent A 1 vial 1 vial   4/-20℃
11．Colour Reagent B1 vial 1 vial   4/-20℃
12．Colour Reagent C  1 vial 1 vial   4/-20℃
13．Instruction    1 set 1 set   RT
Note：
RT：Room temperature
Standard：Frozen dried
Colour Reagent A: Avoid light

【Necessary for testing their own test facilities ａnd equipment】
1. Microplate reader (450nm detection wavelength filter, 570nm or 630nm correction wavelength filters).
2. Washer (adjustable amount of liquid injection to ensure that each well 350μl lotion without overflow).
3. Clean benches, biological safety cabinets, fume hoods.
4. High-precision single-channel dispenser (range 0.5-10μl-20μl, 20-200μl, 200-1000μl).

您可能感兴趣的产品