A1053 IkappaB-alpha (Phospho-Tyr305) Antibody_详细资料

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A1053 IkappaB-alpha （Phospho-Tyr305） Antibody

Reactivity Applications MW. Source

H, M, R WB IHC ELISA 35 kDa Rabbit

Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.

Product Name IkappaB-alpha （Phospho-Tyr305） Antibody

Synonyms I-kappa-B-alpha; IkappaBalpha; IKBA; MAD3; Major histocompatibility complex enhancer-binding protein MAD3; NF-kappaB inhibitor alpha; NFKBI; NFKBIA; RL/IF-1

Immunogen The antiserum was produced against synthesized peptide derived from human IkappaB-alpha around the phosphorylation site of Tyr305.

Immunogen Region C-terminal

Clonality Polyclonal

Modification sites H:Y305 M:Y302 R:Y302

Application dilutions WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

Specificity IkappaB-alpha （Phospho-Tyr305） Antibody detects endogenous levels of IkappaB-alpha only when phosphorylated at tyrosine305.

Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.

Physical Form Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.

Concentration 1 mg/ml

Storage/Stability Store at -20℃/1 year

NCBI Gene Symbol NFKBIA

Database Links

Gene ID: 4792 Swiss-Prot #: P25963

OMIM #: 164008 / 612132 Unigene #: Hs.81328

Application images
Immunohistochemistry analysis of paraffin-embedded human lymph node, using IkappaB-alpha （Phospho-Tyr305） Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from COS7 cells treated with nocodazole 1ug/ml 16h, using IkappaB-alpha （Phospho-Tyr305） Antibody. The lane on the right is treated with the synthesized peptide.

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IkappaB-alpha （Phospho-Tyr305）

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Reactivity	Applications	MW.	Source
H, M, R	WB IHC ELISA	35 kDa	Rabbit

Product Name	IkappaB-alpha （Phospho-Tyr305） Antibody
Synonyms	I-kappa-B-alpha; IkappaBalpha; IKBA; MAD3; Major histocompatibility complex enhancer-binding protein MAD3; NF-kappaB inhibitor alpha; NFKBI; NFKBIA; RL/IF-1
Immunogen	The antiserum was produced against synthesized peptide derived from human IkappaB-alpha around the phosphorylation site of Tyr305.
Immunogen Region	C-terminal
Clonality	Polyclonal
Modification sites	H:Y305 M:Y302 R:Y302
Application dilutions	WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Specificity	IkappaB-alpha （Phospho-Tyr305） Antibody detects endogenous levels of IkappaB-alpha only when phosphorylated at tyrosine305.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Physical Form	Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.
Concentration	1 mg/ml
Storage/Stability	Store at -20℃/1 year