D0008 Histone H3 (Acetyl-Lys18) Antibody_详细资料

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Reactivity Applications MW. Source

H, M, R WB IHC IF ELISA 15 kDa Rabbit

Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.

Product Name Histone H3 （Acetyl-Lys18） Antibody

Synonyms H3/a; H3/c; H3/d; H3/f; H3/h; H3/i; H3/j; H3/k; H3/l; H31; HIST1H3A; histone H3.1

Immunogen The antiserum was produced against synthesized peptide derived from human Histone H3 around the acetylated site of Lys18.

Immunogen Region N-terminal

Clonality Polyclonal

Modification sites H:K18 M:K18 R:K18

Application dilutions WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000

Specificity Histone H3 （Acetyl-Lys18） Antibody detects endogenous levels of total Histone H3 protein only when acetylated at lysine18.

Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acetylation site.

Physical Form Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.

Concentration 1 mg/ml

Storage/Stability Store at -20℃/1 year

NCBI Gene Symbol HIST1H3A / HIST1H3B / HIST1H3C / HIST1H3D / HIST1H3E / HIST1H3F / HIST1H3G / HIST1H3H / HIST1H3I / HIST1H3J / HIST2H3A / HIST2H3C / HIST2H3D / H3F3A / H3F3B

Database Links

Gene ID: 8350 / 8351 / 8352 / 8353 / 8354 / 8355 / 8356 / 8357 / 8358 / 8968 / 126961 / 333932 / 653604 / 3020 / 3023 Swiss-Prot #: P68431 / Q71DI3 / P84243

OMIM #: 602810 / 60281 / 602812 / 602813 / 602814 / 602815 / 602816 / 602817 / 602818 / 602819 / 142780 / 601058 / 601130 Unigene #: Hs.132854 / Hs.247813 / Hs.247814 / Hs.248176 / Hs.443021 / Hs.484990 / Hs.532144 / Hs.533292 / Hs.546315 / Hs.586261 / Hs.591778 / Hs.647745 / Hs.706618 / Hs.712062 / Hs.180877 / Hs.533624 / Hs.723160 / Hs.724423

Application images
Immunofluorescence analysis of HeLa cells, using Histone H3 （Acetyl-Lys18） Antibody. The picture on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Histone H3 （Acetyl-Lys18） Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HeLa cells, treated with TSA 400nM 24h, using Histone H3 （Acetyl-Lys18） Antibody. The lane on the right is treated with the synthesized peptide.

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Reactivity	Applications	MW.	Source
H, M, R	WB IHC IF ELISA	15 kDa	Rabbit

Product Name	Histone H3 （Acetyl-Lys18） Antibody
Synonyms	H3/a; H3/c; H3/d; H3/f; H3/h; H3/i; H3/j; H3/k; H3/l; H31; HIST1H3A; histone H3.1
Immunogen	The antiserum was produced against synthesized peptide derived from human Histone H3 around the acetylated site of Lys18.
Immunogen Region	N-terminal
Clonality	Polyclonal
Modification sites	H:K18 M:K18 R:K18
Application dilutions	WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000
Specificity	Histone H3 （Acetyl-Lys18） Antibody detects endogenous levels of total Histone H3 protein only when acetylated at lysine18.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acetylation site.
Physical Form	Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.
Concentration	1 mg/ml
Storage/Stability	Store at -20℃/1 year