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A0427 FANCD2 (Phospho-Ser222) Antibody
Reactivity | Applications | MW. | Source |
H, M, R | WB IHC ELISA | 166 kDa | Rabbit |
Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.
Product Name | FANCD2 (Phospho-Ser222) Antibody |
Synonyms | FACD2; FANCD2; Fanconi anemia complementation group D2 protein |
Immunogen | The antiserum was produced against synthesized peptide derived from human FANCD2 around the phosphorylation site of Ser222. |
Immunogen Region | N-terminal |
Clonality | Polyclonal |
Modification sites | H:S222 M:S220 R:S222 |
Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 |
Specificity | FANCD2 (Phospho-Ser222) Antibody detects endogenous levels of FANCD2 only when phosphorylated at serine222. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | FANCD2 |
Database Links | Gene ID: | 2177 | Swiss-Prot #: | Q9BXW9 | OMIM #: | 227646 / 227650 | Unigene #: | Hs.208388 | |
Application images | Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using FANCD2 (Phospho-Ser222) Antibody. The picture on the right is treated with the synthesized peptide. Western blot analysis of extracts from HT29 cells treated with Calyculin A 50ng/ml 30', using FANCD2 (Phospho-Ser222) Antibody. The lane on the right is treated with the synthesized peptide. |
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