A0086 SEPARASE (Phospho-Ser801) Antibody_详细资料

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A0086 SEPARASE （Phospho-Ser801） Antibody

Reactivity Applications MW. Source

H, M WB IHC IF ELISA 233 kDa Rabbit

Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.

Product Name SEPARASE （Phospho-Ser801） Antibody

Synonyms ESP1; SEPARIN, EXTRA SPINDLE POLES-LIKE 1, extra spindle poles like 1 （S. cerevisiae）

Immunogen The antiserum was produced against synthesized peptide derived from human SEPARASE around the phosphorylation site of Ser801.

Immunogen Region Internal

Clonality Polyclonal

Modification sites H:S801 M:S1189

Application dilutions WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000

Specificity SEPARASE （Phospho-Ser801） Antibody detects endogenous levels of SEPARASE only when phosphorylated at serine801.

Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.

Physical Form Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.

Concentration 1 mg/ml

Storage/Stability Store at -20℃/1 year

NCBI Gene Symbol ESPL1

Database Links

Gene ID: 9700 Swiss-Prot #: Q14674

OMIM #: 600937 / 601820 / 606176 / 610582 Unigene #: Hs.248141

Application images
Immunofluorescence analysis of HuvEc cells, using SEPARASE （Phospho-Ser801） Antibody. The picture on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using SEPARASE （Phospho-Ser801） Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from 293 cells treated with EGF 200ng/ml 30＇, using SEPARASE （Phospho-Ser801） Antibody. The lane on the right is treated with the synthesized peptide.

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A0086 SEPARASE (Phospho-Ser801) Antibody

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Reactivity	Applications	MW.	Source
H, M	WB IHC IF ELISA	233 kDa	Rabbit

Product Name	SEPARASE （Phospho-Ser801） Antibody
Synonyms	ESP1; SEPARIN, EXTRA SPINDLE POLES-LIKE 1, extra spindle poles like 1 （S. cerevisiae）
Immunogen	The antiserum was produced against synthesized peptide derived from human SEPARASE around the phosphorylation site of Ser801.
Immunogen Region	Internal
Clonality	Polyclonal
Modification sites	H:S801 M:S1189
Application dilutions	WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
Specificity	SEPARASE （Phospho-Ser801） Antibody detects endogenous levels of SEPARASE only when phosphorylated at serine801.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Physical Form	Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.
Concentration	1 mg/ml
Storage/Stability	Store at -20℃/1 year