SigmaL8754 PHA-P植物血球凝集素P 5mg价格公司对外承揽试验:免疫组化, ,Tunel,Elisa,WB,原位杂交,比色法,HE染色,特染,图像分析,荧光欢迎咨询!公司免费代测ELISA试剂盒一周内出结果
SigmaL8754 PHA-P植物血球凝集素P 5mg价格
L8754 | Sigma
外源凝集素(菜豆)
Lectin from Phaseolus vulgaris (red kidney bean)
Phytohemagglutinin PHA-P, lyophilized powder
别名: PHA 植物血球凝集素(菜豆)
SigmaL8754 PHA-P植物血球凝集素P 5mg价格
Analysis Note
Agglutination activity is expressed in mg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation in HeLa cell cultures.
Biochem/physiol Actions
PHA 由两个分子种类构成,一种是红细胞凝集素 (PHA-E),具有低促有丝分裂活性和高红细胞凝集活性,另一种是白细胞凝集素 (PHA-L),具有高促有丝分裂和白细胞凝集活性,但红细胞凝集活性极低。
PHA-E 不具有血型特异性,但凝集作用受某些寡糖YZ。[]PHA-P 是在分离和纯化红细胞凝集素和白细胞凝集素前 PHA 的蛋白质形式。[]PHA-M 为粘蛋白形式。[]由相应的已纯化凝集素制备偶联物。
包装
25, 50 mg in poly bottle
5 mg in glass bottle
Application
Lectin from Phaseolus vulgaris (red kidney bean) is used in phytohemagglutinin (PHA) assay[1] and during assessment of cellular and humoral immunity.[3]
General description
Phytohaemagglutinin (PHA, or phytohemagglutinin) is a lectin found in plants (esp. in legumes). It is obtained from Phaseolus vulgaris (red kidney beans) and acts as a mitogen in human leucocyte culture.[2]