鼠抗人前列腺特异性抗原单克隆抗体(检测)说明书

 

公司可根据客户的不同需要提供经济、快速而可靠的单克隆抗体制备的完整解决方案，本项技术服务包括：

  - Balb/c纯系小鼠免疫；

  - 脾细胞与SP2/0瘤细胞的融合；

  - 抗体产生细胞的ELISA筛选；

  - 抗体产生细胞的亚克隆与扩增；

  - 指定细胞克隆的体外培养或腹水瘤接种法生产单克隆抗体

产品特点
英文名称：Anti-Salmonella typhimurium O/Cy3

别    名：Silent protein UshA(0); USHA_SALTY. 

说 明 书：100ul  

研究领域：细菌及病毒  

抗体来源：Rabbit

克隆类型：Polyclonal

交叉反应：Mouse, SalnellatyphimuriumO

产品应用：ICC=1:50-200 IF=1:50-200  

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量：58kDa

性    状：Lyophilized or Liquid

浓    度：1mg/ml

免 疫 原：whole cell protein of Salmonella typhimurium O

亚    型：IgG

纯化方法：affinity purified by Protein A

储 存 液：Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

保存条件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Histones play a critical role in transcriptional regulation, cell cycle progression, ａnd developmental events. Histone acetylation/deacetylation alters chromosome structure ａnd affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity ａnd represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C ａnd MEF2D. It seems to interact in a multiprotein complex with RbAp48 ａnd HDAC3. [provided by RefSeq, Jul 2008].

 

Function:

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 ａnd H4). Histone deacetylation gives a tag for epigenetic repression ａnd plays an important role in transcriptional regulation, cell cycle progression ａnd developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C ａnd MEF2D.

 

Subunit:

Interacts with HDAC7. Homodimer. Homodimerization via its N-terminal domain. Interacts with MEF2C, AHRR, ａnd NR2C1. Interacts with a 14-3-3 chaperone protein in a phosphorylation dependent manner. Interacts with BTBD14B. Interacts with KDM5B. Interacts with MYOCD. Interacts with MORC2. Interacts with ANKRA2.

 

Subcellular Location:

Nucleus. Cytoplasm. Shuttles between the nucleus ａnd the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein ａnd is due to its phosphorylation at Ser-246, Ser-467 ａnd Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation.

 

Tissue Specificity:

Ubiquitous.

 

Post-translational modifications:

Phosphorylated by CaMK4 at Ser-246, Ser-467 ａnd Ser-632. Phosphorylation at other residues by CaMK2D is required for the interaction with 14-3-3. Phosphorylation at Ser-350 impairs the binding of ANKRA2 but generates a high-affinity docking site for 14-3-3. 

Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, ａnd prevented by phosphorylation by CaMK4.

 

DISEASE:

Defects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, ａnd stocky build. Soft tissue ossification is absent, ａnd there are no abnormalities in parathyroid hormone or calcium metabolism.

 

Similarity:

Belongs to the histone deacetylase family. HD type 2 subfamily.

 

Gene ID:

9759

 

Database links:

Entrez Gene: 9759 Human

Entrez Gene: 208727 Mouse

Entrez Gene: 363287 Rat

Omim: 605314 Human

SwissProt: P56524 Human

SwissProt: Q6NZM9 Mouse

SwissProt: Q99P99 Rat

Unigene: 20516 Human

Unigene: 318567 Mouse

Unigene: 23483 Rat

 

 

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

方法1：我们的抗体（冻干粉）已经包含了0.01M PBS、1% BSA和0.1% 防腐剂（叠氮钠或庆大霉素），您只需要加入灭菌的双蒸水就行了。抗体溶解后，置于-20℃保存，Z好分装后使用，避免反复冻融，可保证至少1年有效；

方法2：也可以只加入一半体积的双蒸水，再用一半体积的甘油补足，置于-20℃保存，这样抗体不会冻，有利于抗体的稳定。

 

后续试验时，再使用对应的缓冲液稀释成工作液，即用即配。

 

我们的抗体（冻干粉），在常温放置下，至少一个月有效；若在-20℃下保存（推荐），至少三年有效。

 

Antibodies are prepared with 0.01M PBS, pH 7.4 with 1% BSA ａnd 0.1% Sodium Azide, prior to lyophilization. Currently, we offer both lyophilized ａnd reconstituted antibodies, both resulting in final concentration of 1mg/mL. We recommend two ways to reconstitute antibodies in lyophilized form:

1) Reconstituting by directly adding 100uL of sterile, distilled water.

2) Alternatively, if glycerol does not affect the experiment, add 50uL sterile distilled water, followed by adding 50uL of glycerol.

 

抗体修FF式

 

修复液：0.01M 枸橼酸（pH6.0）或者0.05M EDTA（pH8.0）

 

方法1：沸水浴修复，将盛有修复液和玻片的烧杯置于沸水浴环境，保持外部沸腾状态15min，自然冷却至室温；

方法2：微波修复，将盛有修复液和玻片的烧杯置于微波炉中，高火5min，停火3min，中火5min，自然冷却至室温。