磷酸化蛋白酪氨酸磷酸酶2抗体说明书

 

公司可根据客户的不同需要提供经济、快速而可靠的单克隆抗体制备的完整解决方案，本项技术服务包括：

  - Balb/c纯系小鼠免疫；

  - 脾细胞与SP2/0瘤细胞的融合；

  - 抗体产生细胞的ELISA筛选；

  - 抗体产生细胞的亚克隆与扩增；

  - 指定细胞克隆的体外培养或腹水瘤接种法生产单克隆抗体

产品特点
英文名称：Anti-Salmonella typhimurium O/Cy3

别    名：Silent protein UshA(0); USHA_SALTY. 

说 明 书：100ul  

研究领域：细菌及病毒  

抗体来源：Rabbit

克隆类型：Polyclonal

交叉反应：Mouse, SalnellatyphimuriumO

产品应用：ICC=1:50-200 IF=1:50-200  

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量：58kDa

性    状：Lyophilized or Liquid

浓    度：1mg/ml

免 疫 原：whole cell protein of Salmonella typhimurium O

亚    型：IgG

纯化方法：affinity purified by Protein A

储 存 液：Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

保存条件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram-negative bacilli that are facultative ａnd flagellated (motile). Salmonellae possess 3 major antigens; the "H" or flagellar antigen (phase 1 & 2), the "O" or somatic antigen (part of the LPS moiety) ａnd the "Vi" or capsular antigen (referred to as "K" in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram-negative bacteria. This LPS is composed of an "O" polysaccharide ("O" antigen) an "R" core ａnd the endotoxic inner "Lipid A". Endotoxins evoke fever ａnd can activate complement, kinin ａnd clotting factors. Until recently the most common cause of food poisoning by Salmonella species was due to S. Typhimurium. As its name suggests, it causes a typhoid-like disease in mice. In humans S. Typhimurium does not cause as severe disease as S. Typhi, ａnd is not normally fatal. The disease is characterized by diarrhea, abdominal cramps, vomiting ａnd nausea, ａnd generally lasts up to 7 days. 

 

Subcellular Location:

Periplasm (By similarity). 

 

Similarity:

Belongs to the 5＇-nucleotidase family.

 

Database links:

UniProtKB/Swiss-Prot: P06196.2

 

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

方法1：我们的抗体（冻干粉）已经包含了0.01M PBS、1% BSA和0.1% 防腐剂（叠氮钠或庆大霉素），您只需要加入灭菌的双蒸水就行了。抗体溶解后，置于-20℃保存，Z好分装后使用，避免反复冻融，可保证至少1年有效；

方法2：也可以只加入一半体积的双蒸水，再用一半体积的甘油补足，置于-20℃保存，这样抗体不会冻，有利于抗体的稳定。

 

后续试验时，再使用对应的缓冲液稀释成工作液，即用即配。

 

我们的抗体（冻干粉），在常温放置下，至少一个月有效；若在-20℃下保存（推荐），至少三年有效。

 

Antibodies are prepared with 0.01M PBS, pH 7.4 with 1% BSA ａnd 0.1% Sodium Azide, prior to lyophilization. Currently, we offer both lyophilized ａnd reconstituted antibodies, both resulting in final concentration of 1mg/mL. We recommend two ways to reconstitute antibodies in lyophilized form:

1) Reconstituting by directly adding 100uL of sterile, distilled water.

2) Alternatively, if glycerol does not affect the experiment, add 50uL sterile distilled water, followed by adding 50uL of glycerol.

 

抗体修FF式

 

修复液：0.01M 枸橼酸（pH6.0）或者0.05M EDTA（pH8.0）

 

方法1：沸水浴修复，将盛有修复液和玻片的烧杯置于沸水浴环境，保持外部沸腾状态15min，自然冷却至室温；

方法2：微波修复，将盛有修复液和玻片的烧杯置于微波炉中，高火5min，停火3min，中火5min，自然冷却至室温。