Nuclistain is intended as a replacement for the conventionally used ethidium bromide for the rapid detection of double ａnd single stranded DNA ａnd RNA in agarose ａnd polyacrylamide gels. Its sensitivity is nearly comparable, with the capability of detecting as little as 10-50 ng of nucleic acid sample. The advantage of Nuclistain is that the results are visible under normal lighting conditions, eliminating the possibility of sample damage by UV radiation. Furthermore, Nuclistain visualization dramatically improves the yield of smaller ａnd medium sized DNA (<500 bp) obtained by purification from agarose. Nuclistain binding is reversible ａnd does not modify nucleic acids.
Nuclistain is extremely easy to use. One 25 ml bottle of Nuclistain will make 2.5 liters of stain solution. The separated DNA appears as dark blue bands on a light blue background.
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| Nuclistain improves the yield of smaller ａnd medium sized DNA (<500 bp) obtained by purification from agarose. One gel was stained with Nuclistain ａnd the other with EtBr. The DNA was subsequently purified from each ａnd then run side by side on a third gel, visualized by EtBr. The results show the improved yield from the Nuclistain. |
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Nuclistain核酸染色剂（EB替代品）
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美国National Diagnostics公司 Nuclistain核酸染色剂（EB替代品）
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