| 产品： 小气道上皮细胞生长因子
货号：3272 Product Description Small Airway Epithelial Cell Growth Supplement （SAEpiCGS） is a medium supplement designed for the optimal growth of normal human Small Airway Epithelial Cells in vitro. It is a sterile, concentrated （100X） solution which contains growth factors, hormones, ａnd proteins necessary for the culture of normal human Small Airway Epithelial Cells. The supplement is formulated （quantitatively ａnd qualitatively） to provide a defined ａnd optimally balanced growth environment that maximally promotes the growth of normal human Small Airway Epithelial Cells in vitro. The supplement is designed as an additive for Small Airway Epithelial Cell medium （SAEpiCM, Cat. No. 3231） ａnd should be used in conjunction with that medium. Components SAEpiCGS is packaged in the quantity of supplement suited for a 500 ml bottle of SAEpiCM. When a 500 ml bottle of SAEpiCM is supplemented with SAEpiCGS, the final concentrations of the supplement components per milliliter will be BSA 5 μg, transferrin 5 μg, BPE 50 μg, insulin 5 mg, FGF-2 5 ng, Epinephrine 500 ng, hydrocortisone 0.5 mg, Prostaglandin E2 10-8 M ａnd T3 30 nM. Note: Due to BPE in the growth supplement, formation of lipoproteins can cause precipitates to be present; the color may vary with different lots. Product Use SAEpiCGS is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. Storage Store SAEpiCGS at -20oC before adding to Small Airway Epithelial Cell medium. Shipping Dry ice. Prepare for use Thaw SAEpiCGS at 37oC. Gently tilt the SAEpiCGS tube several times during thawing to help the contents dissolve. Make sure the contents of the supplement are completely dissolved into solution before adding to the medium. Rinse the bottle ａnd tubes with 70% ethanol, ａnd then wipe to remove excess. Remove the cap, being careful not to touch the interior threads with fingers. Add SAEpiCGS ａnd other component （P/S solution） into medium in a sterile field, mix well ａnd then the reconstituted medium is ready for use. Since several components of SAEpiCM are light-labile, it is recommended that the medium not be exposed to light for lengthy periods of time. If the medium is warmed prior to use, do not exceed 37oC. When stored in the dark at 4oC, the reconstituted medium is stable for one month. |
