大鼠脑脊神经元

产品：大鼠脑脊神经元
货号：R1510
 ScienCell实验室的大鼠脑脊神经元细胞分离于E14大鼠脑脊，原代冻存。冰冻运输或增殖培养后运送。运送前细胞在ScienCell 实验室的神经元培养液中培养2到3天。细胞达到约80％的融合后进行低温运输（在凝胶冰盒中）。它们经检测不含支原体、细菌、酵母菌和真菌。如采用ScienCell 实验室特制的培养基，可保证此神经元在贵实验室中继续生长。    大鼠脑脊神经元细胞（RN-r）提取于新生E14大鼠脑脊，每管含有细胞数>1×106 cells/ml，原代冻存。运送前置入神经细胞培养基中培养2-3天，经测试不含有支原体、细菌、酵母和真菌。推荐培养基:（NM, Cat. No. 1521）Description

 

The tissue of the central nervous system is made up of two classes of cells that may be broadly categorized as neurons ａnd glia. Neurons are anatomic, functional, ａnd trophic units of the brain [1]. Despite great variability in size ａnd shape, all neurons share common morphological features, which are those of the key elements of a highly complex communication network. The neurons are the dynamically polarized cells that serve as the major signaling unit of the nervous system.

The RN-r from ScienCell Research Laboratories are isolated from E14 rat brain striatum. RN-r are cryopreserved at primary cultures ａnd delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. RN-r are characterized by immunofluorescent method with antibodies to neurafilament, MAP2, ａnd beta-tubulin 3. RN-r are negative for mycoplasma, bacteria, yeast ａnd fungi. RN-r are guaranteed to further culture in the conditions provided by ScienCell Research Laboratories.
Recommended Medium

 

It is recommended to use Neuronal Medium （NM, Cat. No. 1521） for the culture of rat neurons in vitro.
Product Use

 

RN-r are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage

 

Transfer cells directly ａnd immediately from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture is needed for experiments.
Shipping

 

Dry ice.
Reference

 

[1] Parent, A. （1996） Neurons in Carpenter＇s Human Neuroanatomy. 9th ed., pp131-198, Williams & Wilkins, Quebec, Canada.
[2] Alberts, B., Bray, D., Lewis, J., Raff, M., Roberts, M., Watson, J. D. （1989） Molecular biology of the cell. 2nd. ed., New York: Garland.