人绒毛膜毛细血管内皮细胞

人绒毛膜毛细血管内皮细胞说明：
货号：7100

Cell Specification
Human amniotic membrane is composed of an epithelial cell layer, a basement membrane ａnd an
avascular matrix. The amniotic epithelial cells （AEC） are formed from epiblasts on the 8th day
after    fertilization.    A    probable     result   of   their   embryonic       origin,   AEC      lack    major
histocompatibility complex antigens ａnd have been used for allotranplantation to treat patients
with lysosomal diseases. Studies have shown that AEC have multiple functions such as synthesis
and    release   of  acetylcholine     and   catecholamine      as  well   as  expressing    mRNA       coding   for
dopamine   receptors   and   dopamine   transporter   [1].  They   express   neuronal   and   glial   cell   marks
and produce basic fibroblast growth factor, hepatocyte growth factor ａnd transforming growth
factor-beta [2]. Human AEC has been suggested as an appropriate human cell model for studying
dopamine       release  and   uptake    processes,    receptor   signal   transduction    and    exploring    newly
developed drugs acting at these receptors [3].

HAEpiC   from   ScienCell   Research   Laboratories   are   isolated   from   human   amniotic   membrane.
HAEpiC are cryopreserved at passage one ａnd delivered frozen. Each vial contains >5 x 105 cells

in   1   ml   volume.   HAEpiC   are   characterized   by  immunofluorescent   method   with   antibodies   to
cytokeratin-18,   -19   and   vimentin.   HAEpiC   are negative   for   HIV-1,   HBV,   HCV,   mycoplasma,
bacteria, yeast ａnd fungi. HAEpiC are guaranteed to further expand for 15 population doublings
in the condition provided by ScienCell Research Laboratories.

Recommended Medium
It is recommended to use Epithelial Cell Medium （EpiCM, Cat. No. 4101） for the culturing of
HAEpiC in vitro.

Product Use
HAEpiC are for research use only. It is not approved for human or animal use, or for application
in in vitro diagnostic procedures.

Storage
Directly ａnd immediately transfer cells from dry ice to liquid nitrogen upon receiving ａnd keep
the cells in liquid nitrogen until cell culture is needed for experiments.

Shipping
Dry ice.

Reference
[1] Sakuragawa, N., Elwan, M. A., Uchida, S., Fujii, T., Kawashima, K. （2001） Non-neuronal neurotransmitters ａnd
neurotrophic factors in amniotic epithelial cells: expression ａnd function in humans ａnd monkey. Jpn J Pharmacol.
85（1）:20-3.
[2]   Ishii,   T.,   Ohsugi,   K.,   Nakamura,   S.,   Sato,   K.,   Hashimoto,   M.,   Mikoshiba,   K.,   Sakuragawa,   N.   （1999）   Gene
expression of oligodendrocyte markers in human amniotic epithelial cells using neural cell-type-specific expression
system. Neurosci Lett. 268（3）:131-4.
[3] Elwan, M. A., Ishii, T., Sakuragawa, N. （2003） Characterization of the dopamine transporter gene expression ａnd
binding sites in cultured human amniotic epithelial cells. Neurosci Lett. 342（1-2）:61-4.